Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS31695 and BPHYT_RS31700 are separated by 538 nucleotidesBPHYT_RS31700 and BPHYT_RS31705 are separated by 37 nucleotidesBPHYT_RS31705 and BPHYT_RS31710 are separated by 123 nucleotidesBPHYT_RS31710 and BPHYT_RS31715 are separated by 133 nucleotides BPHYT_RS31695: BPHYT_RS31695 - dihydroxy-acid dehydratase, at 2,666,178 to 2,667,965 _RS31695 BPHYT_RS31700: BPHYT_RS31700 - porin, at 2,668,504 to 2,669,583 _RS31700 BPHYT_RS31705: BPHYT_RS31705 - MFS transporter, at 2,669,621 to 2,670,934 _RS31705 BPHYT_RS31710: BPHYT_RS31710 - porin, at 2,671,058 to 2,672,203 _RS31710 BPHYT_RS31715: BPHYT_RS31715 - branched-chain amino acid ABC transporter substrate-binding protein, at 2,672,337 to 2,673,479 _RS31715
Group Condition BPHYT_RS31695 BPHYT_RS31700 BPHYT_RS31705 BPHYT_RS31710 BPHYT_RS31715
phage 24hr PHYTO3 MOI 10 -0.3 -1.5 -0.1 -0.0 -0.7
phage P. phyto PHYTO2 MOI 1 +0.0 -2.7 +0.1 -0.1 +0.2
tn7 efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pRH19; outgrowth in LB_gent50 -0.1 -1.4 +0.2 -0.2 -0.9
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.1 -0.5 -0.1 -0.8 -1.0
phage 36hr PHYTO8 MOI 0.1 -0.3 -1.2 -0.2 -0.1 -0.4
in planta Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days +0.1 -0.5 -0.4 -0.4 -1.0
phage 24hr PHYTO6 MOI 10 -0.7 -0.8 -0.2 -0.2 -0.3
nophagecontrol 24hr Phyto Lib Only +0.3 -1.2 -0.0 -0.3 -0.9
phage 24hr PHYTO6 MOI 1 -0.3 -1.1 +0.2 -0.1 -0.6
phage 36hr PHYTO7 MOI 1 -0.2 -1.1 +0.2 -0.6 -0.2
in planta Rhizosphere from sorghum RTx_430 plants, grown 2 months, low phosphate; Rep 1 -0.2 +0.3 -0.0 -0.2 -1.7
replicable vector efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 -0.1 -0.7 +0.2 +0.1 -1.3
in planta Plant=Zea_mays; PlantTreatment=None; Sample=soil; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.4 -0.5 -0.6 -0.1 -1.0
phage 24hr PHYTO MOI 0.1 -0.2 -1.2 +0.1 +0.2 -0.6
replicable vector efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 -0.1 -1.0 -0.3 +0.2 -0.4
phage 36hr PHYTO1 MOI 1 +0.2 -1.8 -0.1 +0.1 +0.0
phage 36hr PHYTO6 MOI 1 -0.2 -1.4 +0.2 +0.1 -0.1
phage 36hr PHYTO11 MOI 1 +0.2 -1.5 +0.2 -0.1 +0.0
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.5 -0.8 -0.1 +0.1 -0.6
in planta Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.7 -0.7 -0.1 -0.2 -0.3
phage 24hr PHYTO3 MOI 10 +0.4 -1.4 +0.4 -0.0 +0.2
phage 36hr PHYTO8 MOI 10 +0.6 -0.4 +0.1 +0.2 -1.0
phage 36hr PHYTO2 MOI 1 +0.4 -0.9 -0.2 -0.2 +0.6
phage 36hr PHYTO10 MOI 10 +0.4 -0.8 +0.2 -0.4 +0.3
phage 24hr PHYTO7 MOI 10 -0.3 -0.8 -0.1 +0.5 +0.7
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.1 -0.9 +0.2 +0.4 +0.8
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.2 -0.5 +0.7 +0.1 +0.5
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.8X; growth supplemented with 0.4X LB +0.2 +0.9 +0.4 +0.3 -0.3
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB +0.8 +0.8 +0.6 -0.1 +0.3
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB +0.4 +0.6 +0.3 +0.6 +1.4
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BPHYT_RS31695
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BPHYT_RS31700
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BPHYT_RS31715
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