Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS31655 and BPHYT_RS31660 are separated by 19 nucleotidesBPHYT_RS31660 and BPHYT_RS31665 are separated by 121 nucleotidesBPHYT_RS31665 and BPHYT_RS31670 are separated by 1191 nucleotidesBPHYT_RS31670 and BPHYT_RS31675 are separated by 298 nucleotides BPHYT_RS31655: BPHYT_RS31655 - glycolate oxidase, at 2,656,720 to 2,657,949 _RS31655 BPHYT_RS31660: BPHYT_RS31660 - hypothetical protein, at 2,657,969 to 2,658,373 _RS31660 BPHYT_RS31665: BPHYT_RS31665 - malate synthase, at 2,658,495 to 2,660,669 _RS31665 BPHYT_RS31670: BPHYT_RS31670 - transposase, at 2,661,861 to 2,662,196 _RS31670 BPHYT_RS31675: BPHYT_RS31675 - hypothetical protein, at 2,662,495 to 2,662,821 _RS31675
Group Condition BPHYT_RS31655 BPHYT_RS31660 BPHYT_RS31665 BPHYT_RS31670 BPHYT_RS31675
carbon source 2-Deoxy-D-ribonic lithium salt (C) -0.1 N.D. N.D. -1.3 -0.5
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.2 N.D. N.D. -0.6 -1.0
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.3 N.D. N.D. -0.5 -1.0
phage 24hr PHYTO8 MOI 1 -0.1 N.D. N.D. -0.4 -1.1
supernatant control:fungal media R2A with Vogels_fungal_media 0.8X +0.0 N.D. N.D. -0.4 -1.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant +0.0 N.D. N.D. -0.3 -1.1
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth; Time=14_days -0.4 N.D. N.D. -0.9 -0.2
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.3 N.D. N.D. -0.3 -0.7
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant +0.3 N.D. N.D. -1.2 +0.0
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.7 N.D. N.D. -0.7 -0.9
in planta Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.3 N.D. N.D. -0.7 -0.3
nophagecontrol 36hr Phyto Lib only -0.1 N.D. N.D. +0.4 -0.9
carbon source Phenylacetic 5 mM (C) +0.1 N.D. N.D. +0.5 -1.0
in planta Plant=Arabidopsis_thaliana_FLS2; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_0.8%agar; Collection=outgrowth_LB; Time=7_days +0.3 N.D. N.D. -0.9 +0.2
in planta Plant=Zea_mays; PlantTreatment=None; Sample=soil; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.8 N.D. N.D. -0.2 -1.0
phage 36hr PHYTO9 MOI 0.1 -0.6 N.D. N.D. +0.9 -0.5
phage 24hr PHYTO3 MOI 10 -0.4 N.D. N.D. -0.6 +0.8
phage P. phyto PHYTO2 MOI 1 +0.1 N.D. N.D. -0.9 +0.6
phage 36hr PHYTO11 MOI 1 +0.2 N.D. N.D. +0.5 -0.8
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -0.2 N.D. N.D. +0.8 -0.5
phage 36hr PHYTO10 MOI 10 -0.5 N.D. N.D. -0.1 +0.8
carbon source Tween 20 (C) +0.4 N.D. N.D. +0.6 -0.6
motility inner cut, LB soft agar motility assay -0.3 N.D. N.D. -0.4 +1.2
phage 36hr PHYTO4 MOI 1 +0.5 N.D. N.D. +0.6 -0.4
phage 24hr PHYTO6 MOI 0.1 -0.4 N.D. N.D. +1.0 +0.1
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB +0.5 N.D. N.D. -0.4 +0.6
carbon source Malonic (C) +0.1 N.D. N.D. -0.2 +1.5
carbon source Deoxyribonucleic from herring sperm 10 mg/mL (C) +0.6 N.D. N.D. +0.1 +0.6
phage 36hr PHYTO8 MOI 10 +0.5 N.D. N.D. +0.3 +0.8
nitrogen source L-Proline (N) +0.1 N.D. N.D. +0.3 +1.2
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