Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS31505 and BPHYT_RS31510 are separated by 1022 nucleotidesBPHYT_RS31510 and BPHYT_RS31515 are separated by 346 nucleotidesBPHYT_RS31515 and BPHYT_RS31520 are separated by 595 nucleotidesBPHYT_RS31520 and BPHYT_RS31525 are separated by 477 nucleotides BPHYT_RS31505: BPHYT_RS31505 - hypothetical protein, at 2,620,268 to 2,621,038 _RS31505 BPHYT_RS31510: BPHYT_RS31510 - sulfonate ABC transporter substrate-binding protein, at 2,622,061 to 2,623,434 _RS31510 BPHYT_RS31515: BPHYT_RS31515 - agmatinase, at 2,623,781 to 2,624,755 _RS31515 BPHYT_RS31520: BPHYT_RS31520 - LysR family transcriptional regulator, at 2,625,351 to 2,626,265 _RS31520 BPHYT_RS31525: BPHYT_RS31525 - hypothetical protein, at 2,626,743 to 2,627,024 _RS31525
Group Condition BPHYT_RS31505 BPHYT_RS31510 BPHYT_RS31515 BPHYT_RS31520 BPHYT_RS31525
in planta Plant=Zea_mays; PlantTreatment=Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days -0.2 -1.6 -1.4 -0.4 -0.1
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.2 +0.0 -2.7 -0.4 -0.4
replicable vector efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 -0.4 -0.8 -1.1 -0.2 -0.3
in planta Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -1.0 +0.6 -0.9 -0.5 -0.9
in planta Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days -1.0 +0.4 -1.7 +0.1 -0.3
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.5X; growth supplemented with 0.4X LB -0.4 +0.4 -1.0 +0.2 -1.8
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth; Time=14_days +0.5 +0.2 -1.8 -0.3 -0.8
phage 24hr PHYTO2 MOI 0.1 -1.2 -0.2 -0.6 -0.4 +0.5
phage P. phyto corn MOI 1 -0.8 -1.6 +0.4 -0.0 +0.1
phage 24hr PHYTO3 MOI 10 +0.6 +0.2 -2.7 -0.5 +0.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant +0.3 -1.4 -0.7 -0.2 +0.3
in planta Plant=Zea_mays; PlantTreatment=None; Sample=soil; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.9 +0.7 +0.2 -0.6 -0.5
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.4 +0.8 -1.4 +0.4 -0.4
nophagecontrol 36hr Phyto Lib only +0.9 -0.6 +0.1 -1.1 +0.4
phage 24hr PHYTO11 MOI 1 -0.6 +0.3 +0.7 -1.0 +0.6
phage 36hr PHYTO3 MOI 0.1 +0.4 -0.5 +1.1 -0.3 -0.5
phage 36hr PHYTO4 MOI 0.1 +0.3 -1.0 +0.9 -0.3 +0.5
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days -0.2 +0.4 +1.5 -1.4 +0.2
phage 36hr PHYTO2 MOI 1 -0.4 -0.4 +0.7 -0.4 +1.0
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB -0.9 +1.3 -0.2 -0.4 +0.7
nophagecontrol 24hr Phyto Lib Only -0.1 -0.9 +1.2 -0.2 +0.7
phage 36hr PHYTO8 MOI 10 +0.6 -0.4 +1.4 -0.0 -0.5
supernatant control:fungal media R2A with Vogels_fungal_media 0.8X +0.6 -0.2 +1.5 +0.1 -0.5
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.3 +0.1 +2.1 +0.2 -0.7
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days +0.5 -0.0 +1.8 +0.1 -0.8
in planta Rhizosphere from sorghum RTx_430 plants, grown 2 months, low phosphate; Rep 1 +0.5 -0.1 +2.1 -0.4 -0.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant +1.3 -0.2 +1.0 +0.1 -0.4
phage 36hr PHYTO6 MOI 1 +0.7 -0.5 +1.2 -0.2 +0.7
motility outer cut, LB soft agar motility assay +0.6 -0.3 +1.1 +0.1 +1.0
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB -0.1 +1.0 +0.4 +0.7 +1.0
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