Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS31370 and BPHYT_RS31375 are separated by 596 nucleotidesBPHYT_RS31375 and BPHYT_RS31380 are separated by 102 nucleotidesBPHYT_RS31380 and BPHYT_RS31385 are separated by 63 nucleotidesBPHYT_RS31385 and BPHYT_RS31390 overlap by 7 nucleotides BPHYT_RS31370: BPHYT_RS31370 - hypothetical protein, at 2,592,973 to 2,593,179 _RS31370 BPHYT_RS31375: BPHYT_RS31375 - AraC family transcriptional regulator, at 2,593,776 to 2,594,693 _RS31375 BPHYT_RS31380: BPHYT_RS31380 - dehydrogenase, at 2,594,796 to 2,595,545 _RS31380 BPHYT_RS31385: BPHYT_RS31385 - ketoacyl reductase, at 2,595,609 to 2,596,367 _RS31385 BPHYT_RS31390: BPHYT_RS31390 - hypothetical protein, at 2,596,361 to 2,596,591 _RS31390
Group Condition BPHYT_RS31370 BPHYT_RS31375 BPHYT_RS31380 BPHYT_RS31385 BPHYT_RS31390
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.5X; growth supplemented with 0.4X LB N.D. -0.8 N.D. -1.7 -0.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant N.D. -0.5 N.D. -2.3 +0.0
phage 36hr PHYTO4 MOI 1 N.D. -0.0 N.D. -1.9 -0.4
carbon source D-Glucose 20 mM (C) N.D. -0.3 N.D. -1.0 -0.9
phage 24hr PHYTO7 MOI 10 N.D. -1.1 N.D. -0.5 -0.5
carbon source D-Glucose 20 mM (C) N.D. -0.5 N.D. -0.5 -0.7
phage 24hr PHYTO9 MOI 0.1 N.D. -0.2 N.D. -0.9 -0.6
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant N.D. +0.6 N.D. -1.7 -0.3
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.8X; growth supplemented with 0.4X LB N.D. +0.6 N.D. -1.1 -0.8
replicable vector efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 N.D. -0.4 N.D. +0.2 -1.1
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth; Time=14_days N.D. +0.4 N.D. -0.9 -0.6
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB N.D. -0.6 N.D. -0.8 +0.4
phage 36hr PHYTO8 MOI 10 N.D. +0.7 N.D. -1.4 -0.2
motility outer cut, LB soft agar motility assay N.D. -0.7 N.D. -0.6 +0.5
phage 36hr PHYTO10 MOI 10 N.D. -0.1 N.D. +0.4 -1.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant N.D. +0.4 N.D. -1.3 +0.3
motility outer cut, LB soft agar motility assay N.D. -0.3 N.D. -1.1 +0.7
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth; Time=14_days N.D. +0.5 N.D. -1.1 -0.0
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB N.D. +0.5 N.D. -1.3 +0.2
nophagecontrol 24hr Phyto Lib Only N.D. +0.3 N.D. +0.2 -1.0
phage 36hr PHYTO7 MOI 1 N.D. +0.6 N.D. +0.3 -0.9
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. -0.7 N.D. +1.0 +0.0
phage 24hr PHYTO MOI 0.1 N.D. +0.3 N.D. +0.7 -0.6
carbon source D-Fructose (C) N.D. +0.6 N.D. +0.7 -0.3
phage 36hr PHYTO1 MOI 10 N.D. +0.6 N.D. +0.9 -0.5
phage 36hr PHYTO8 MOI 10 N.D. +0.7 N.D. +1.0 -0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. +0.8 N.D. -0.2 +0.8
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. +0.5 N.D. +1.2 -0.1
carbon source 2-Deoxy-D-ribonic lithium salt (C) N.D. +0.4 N.D. +1.1 +0.5
carbon source Malonic (C) N.D. +1.4 N.D. +0.9 +0.2
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BPHYT_RS31370
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BPHYT_RS31375
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