Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS31180 and BPHYT_RS31185 are separated by 124 nucleotidesBPHYT_RS31185 and BPHYT_RS31190 are separated by 141 nucleotidesBPHYT_RS31190 and BPHYT_RS31195 overlap by 4 nucleotidesBPHYT_RS31195 and BPHYT_RS31200 are separated by 8 nucleotides BPHYT_RS31180: BPHYT_RS31180 - DGPFAETKE family protein, at 2,553,792 to 2,554,163 _RS31180 BPHYT_RS31185: BPHYT_RS31185 - LysR family transcriptional regulator, at 2,554,288 to 2,555,199 _RS31185 BPHYT_RS31190: BPHYT_RS31190 - ABC transporter permease, at 2,555,341 to 2,556,312 _RS31190 BPHYT_RS31195: BPHYT_RS31195 - peptide ABC transporter permease, at 2,556,309 to 2,557,220 _RS31195 BPHYT_RS31200: BPHYT_RS31200 - peptide ABC transporter substrate-binding protein, at 2,557,229 to 2,558,206 _RS31200
Group Condition BPHYT_RS31180 BPHYT_RS31185 BPHYT_RS31190 BPHYT_RS31195 BPHYT_RS31200
nitrogen source L-Lysine (N) N.D. -0.7 -0.0 -0.3 -1.5
stress Bacitracin 0.25 mg/ml N.D. -0.3 -0.5 -0.9 -0.7
phage 36hr PHYTO1 MOI 1 N.D. -0.2 -0.3 -1.2 -0.4
phage 36hr PHYTO6 MOI 10 N.D. -0.6 -0.0 -1.0 -0.1
phage 36hr PHYTO10 MOI 10 N.D. -0.1 -0.6 -0.8 -0.3
phage P. phyto PHYTO2 MOI 1 N.D. -0.6 +0.1 -0.5 -0.7
phage 24hr PHYTO10 MOI 1 N.D. +0.3 -0.4 -0.9 -0.5
carbon source Phenylacetic 5 mM (C) N.D. -1.1 +0.2 -0.4 +0.0
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth; Time=14_days N.D. -0.5 -0.1 +0.5 -0.7
phage PHYTO3 MOI 0.1 N.D. +0.2 +0.2 -0.9 -0.3
nophagecontrol 36hr Phyto Lib only N.D. -0.4 -0.1 -0.7 +0.5
carbon source Suberic (C) N.D. -0.2 -0.5 -0.5 +0.5
in planta Rhizosphere from sorghum RTx_430 plants, grown 2 months, low phosphate; Rep 1 N.D. -0.2 -0.7 +0.6 -0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant N.D. -0.5 +0.1 +0.6 -0.6
phage 36hr PHYTO4 MOI 1 N.D. +0.1 +0.4 -1.0 +0.2
vitamin No vitamins t2 N.D. +0.3 -0.2 +0.5 -0.8
in planta Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. +0.4 -0.9 +0.6 -0.1
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB N.D. +0.4 -0.9 +0.4 +0.1
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=14_days N.D. +0.1 -0.7 +0.3 +0.6
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant N.D. +0.6 -0.3 -0.4 +0.4
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days N.D. +0.1 +1.0 -0.2 -0.5
in planta Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. +0.4 -0.6 +0.5 +0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant N.D. -0.2 +0.5 -0.4 +0.8
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days N.D. +0.3 +0.8 -0.4 +0.2
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days N.D. +0.4 +1.1 -0.1 -0.2
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB N.D. +0.6 +0.5 -0.3 +0.5
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. +0.3 +1.0 -0.5 +0.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. +0.8 -0.2 +0.7 +0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. +0.2 +0.5 -0.2 +1.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant N.D. +0.2 +0.6 +0.1 +1.0
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