Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS31155 and BPHYT_RS31160 are separated by 148 nucleotidesBPHYT_RS31160 and BPHYT_RS31165 are separated by 154 nucleotidesBPHYT_RS31165 and BPHYT_RS31170 are separated by 1237 nucleotidesBPHYT_RS31170 and BPHYT_RS31175 are separated by 145 nucleotides BPHYT_RS31155: BPHYT_RS31155 - AraC family transcriptional regulator, at 2,546,132 to 2,547,094 _RS31155 BPHYT_RS31160: BPHYT_RS31160 - GCN5 family acetyltransferase, at 2,547,243 to 2,547,962 _RS31160 BPHYT_RS31165: BPHYT_RS31165 - hypothetical protein, at 2,548,117 to 2,549,310 _RS31165 BPHYT_RS31170: BPHYT_RS31170 - hypothetical protein, at 2,550,548 to 2,552,365 _RS31170 BPHYT_RS31175: BPHYT_RS31175 - RNA polymerase sigma70 factor, at 2,552,511 to 2,553,785 _RS31175
Group Condition BPHYT_RS31155 BPHYT_RS31160 BPHYT_RS31165 BPHYT_RS31170 BPHYT_RS31175
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 1X +0.1 -0.8 +0.1 -0.7 -0.2
phage 24hr PHYTO9 MOI 0.1 -0.4 -0.7 -0.1 -0.3 +0.2
phage 24hr PHYTO8 MOI 1 -1.1 -0.1 -0.2 -0.1 +0.2
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.8X; growth supplemented with 0.4X LB -0.6 -0.1 +0.2 -0.2 -0.5
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.3 -0.5 +0.2 -0.5 -0.0
phage 36hr PHYTO7 MOI 0.1 -0.3 -0.8 +0.2 -0.4 +0.2
supernatant control Vogels_fungal_media; pH 5.5 +0.1 +0.1 -0.5 -0.5 -0.3
carbon source Phenylacetic 5 mM (C) -1.1 +0.5 -0.2 +0.1 -0.4
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.5X; growth supplemented with 0.4X LB -0.1 -0.3 +0.4 -0.6 -0.4
nitrogen source L-Lysine (N) -1.1 +0.7 -0.6 +0.2 +0.0
in planta Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.9 -0.3 +0.2 +0.0 +0.2
phage 24hr PHYTO3 MOI 10 +0.3 -0.8 +0.2 -0.2 -0.2
phage 24hr PHYTO7 MOI 1 -1.0 -0.2 +0.1 +0.0 +0.4
phage 36hr PHYTO3 MOI 1 -0.4 -0.8 +0.1 +0.2 +0.3
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth; Time=14_days -0.4 -0.7 +0.3 +0.2 +0.4
phage 36hr PHYTO10 MOI 10 +0.1 -0.7 +0.5 +0.1 -0.2
motility outer cut, LB soft agar motility assay -0.8 -0.3 +0.2 +0.5 +0.2
carbon source 2-Deoxy-D-ribonic lithium salt (C) -0.8 -0.2 +0.2 +0.2 +0.6
in planta Rhizosphere from sorghum RTx_430 plants, grown 2 months, low phosphate; Rep 1 -0.7 +0.6 -0.1 +0.0 +0.4
carbon source Suberic (C) -0.6 +0.8 -0.1 -0.0 +0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.6 +0.0 +0.5 +0.7 -0.2
carbon source Azelaic (C) -0.4 +0.6 -0.0 +0.4 -0.2
phage 36hr PHYTO4 MOI 1 +0.3 -0.5 -0.2 +0.2 +0.5
phage 24hr PHYTO2 MOI 0.1 +0.5 -0.4 -0.1 -0.1 +0.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant +0.1 -0.3 -0.3 +0.5 +0.6
phage 36hr PHYTO11 MOI 0.1 +0.8 -0.4 -0.0 +0.1 +0.3
in planta Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 -0.0 -0.2 +0.9 +0.3 -0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant +0.4 +0.5 -0.4 -0.1 +0.5
phage P. phyto PHYTO2 MOI 0.1 -0.2 +0.7 +0.2 +0.2 -0.2
in planta Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.0 +0.3 +0.4 +0.3 +0.6
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