Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS31135 and BPHYT_RS31140 are separated by 46 nucleotidesBPHYT_RS31140 and BPHYT_RS31145 are separated by 220 nucleotidesBPHYT_RS31145 and BPHYT_RS31150 are separated by 208 nucleotidesBPHYT_RS31150 and BPHYT_RS31155 are separated by 279 nucleotides BPHYT_RS31135: BPHYT_RS31135 - hexuranate MFS transporter, at 2,540,780 to 2,542,069 _RS31135 BPHYT_RS31140: BPHYT_RS31140 - MFS transporter, at 2,542,116 to 2,543,444 _RS31140 BPHYT_RS31145: BPHYT_RS31145 - glucarate dehydratase, at 2,543,665 to 2,544,939 _RS31145 BPHYT_RS31150: BPHYT_RS31150 - transcriptional regulator, at 2,545,148 to 2,545,852 _RS31150 BPHYT_RS31155: BPHYT_RS31155 - AraC family transcriptional regulator, at 2,546,132 to 2,547,094 _RS31155
Group Condition BPHYT_RS31135 BPHYT_RS31140 BPHYT_RS31145 BPHYT_RS31150 BPHYT_RS31155
phage 24hr PHYTO2 MOI 1 -0.4 -0.6 -0.4 -1.0 -0.1
phage 24hr PHYTO10 MOI 1 -0.6 -0.5 -0.2 -1.3 -0.0
phage 36hr PHYTO8 MOI 0.1 -0.5 -0.5 +0.0 -1.3 -0.1
carbon source Suberic (C) -0.1 -0.6 -0.2 -0.9 -0.6
phage P. phyto PHYTO2 MOI 0.1 -0.0 -0.2 +0.1 -2.1 -0.2
phage 36hr PHYTO2 MOI 1 -0.9 -0.1 +0.0 -1.1 -0.3
motility inner cut, LB soft agar motility assay -0.1 -0.3 -0.5 -1.2 -0.2
phage 24hr PHYTO11 MOI 0.1 -0.6 -0.1 -0.2 -0.7 -0.6
phage 36hr PHYTO3 MOI 0.1 -0.2 -0.4 -0.6 -0.7 -0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -0.4 -0.6 -0.1 -0.6 -0.4
phage 36hr PHYTO11 MOI 1 -1.0 -0.3 -0.1 -0.5 -0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant -0.1 -0.8 +0.1 -0.9 -0.5
phage P. phyto PHYTO2 MOI 1 +0.3 -0.1 -0.3 -1.4 -0.5
nitrogen source L-Isoleucine (N) -0.2 -0.7 -0.2 -1.1 +0.1
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.5X; growth supplemented with 0.4X LB -0.5 -0.1 -0.8 -0.6 +0.1
phage P. phyto PHYTO2 MOI 1 -0.3 +0.1 -0.4 -1.3 -0.0
phage 24hr PHYTO8 MOI 1 -0.6 -0.3 -0.1 +0.3 -1.1
phage 24hr PHYTO1 MOI 1 -0.3 -0.5 -0.1 -1.0 +0.2
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.4 -0.2 -0.5 -1.0 -0.3
phage 24hr PHYTO MOI 0.1 -0.6 -0.5 +0.1 -0.6 +0.3
in planta Rhizosphere from sorghum RTx_430 plants, grown 2 months, low phosphate; Rep 1 -0.1 -0.3 +0.4 -0.6 -0.7
phage 24hr PHYTO6 MOI 1 -0.2 -0.3 -0.9 -0.3 +0.4
phage 24hr PHYTO3 MOI 10 -1.2 +0.4 -0.5 -0.4 +0.4
phage 24hr PHYTO10 MOI 10 -0.5 -0.2 -0.1 -0.8 +0.4
motility outer cut, LB soft agar motility assay +0.4 +0.5 -0.5 -0.9 -0.8
nophagecontrol 36hr Phyto Lib only -0.3 -0.5 +0.1 -0.8 +0.4
phage 36hr PHYTO9 MOI 0.1 -0.3 -0.3 +0.3 -1.0 +0.3
carbon source Phenylacetic 5 mM (C) +0.5 -0.3 +0.1 -0.3 -1.1
phage 24hr PHYTO4 MOI 0.1 -0.5 -0.2 -0.3 -0.3 +0.9
phage 36hr PHYTO1 MOI 10 -0.4 -0.3 -0.4 +0.9 +0.4
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