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Protein
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Fitness for 5 genes in
Burkholderia phytofirmans PsJN
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Top 30 experiments (either direction), sorted by average fitness
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500 nt
BPHYT_RS31135 and BPHYT_RS31140 are separated by 46 nucleotides
BPHYT_RS31140 and BPHYT_RS31145 are separated by 220 nucleotides
BPHYT_RS31145 and BPHYT_RS31150 are separated by 208 nucleotides
BPHYT_RS31150 and BPHYT_RS31155 are separated by 279 nucleotides
BPHYT_RS31135: BPHYT_RS31135 - hexuranate MFS transporter, at 2,540,780 to 2,542,069
_RS31135
BPHYT_RS31140: BPHYT_RS31140 - MFS transporter, at 2,542,116 to 2,543,444
_RS31140
BPHYT_RS31145: BPHYT_RS31145 - glucarate dehydratase, at 2,543,665 to 2,544,939
_RS31145
BPHYT_RS31150: BPHYT_RS31150 - transcriptional regulator, at 2,545,148 to 2,545,852
_RS31150
BPHYT_RS31155: BPHYT_RS31155 - AraC family transcriptional regulator, at 2,546,132 to 2,547,094
_RS31155
Group
Condition
BPHYT
_RS31135
BPHYT
_RS31140
BPHYT
_RS31145
BPHYT
_RS31150
BPHYT
_RS31155
phage
24hr PHYTO2 MOI 1
-0.4
-0.6
-0.4
-1.0
-0.1
phage
24hr PHYTO10 MOI 1
-0.6
-0.5
-0.2
-1.3
-0.0
phage
36hr PHYTO8 MOI 0.1
-0.5
-0.5
+0.0
-1.3
-0.1
carbon source
Suberic (C)
-0.1
-0.6
-0.2
-0.9
-0.6
phage
P. phyto PHYTO2 MOI 0.1
-0.0
-0.2
+0.1
-2.1
-0.2
phage
36hr PHYTO2 MOI 1
-0.9
-0.1
+0.0
-1.1
-0.3
motility
inner cut, LB soft agar motility assay
-0.1
-0.3
-0.5
-1.2
-0.2
phage
24hr PHYTO11 MOI 0.1
-0.6
-0.1
-0.2
-0.7
-0.6
phage
36hr PHYTO3 MOI 0.1
-0.2
-0.4
-0.6
-0.7
-0.2
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
-0.4
-0.6
-0.1
-0.6
-0.4
phage
36hr PHYTO11 MOI 1
-1.0
-0.3
-0.1
-0.5
-0.3
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant
-0.1
-0.8
+0.1
-0.9
-0.5
phage
P. phyto PHYTO2 MOI 1
+0.3
-0.1
-0.3
-1.4
-0.5
nitrogen source
L-Isoleucine (N)
-0.2
-0.7
-0.2
-1.1
+0.1
supernatant
Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.5X; growth supplemented with 0.4X LB
-0.5
-0.1
-0.8
-0.6
+0.1
phage
P. phyto PHYTO2 MOI 1
-0.3
+0.1
-0.4
-1.3
-0.0
phage
24hr PHYTO8 MOI 1
-0.6
-0.3
-0.1
+0.3
-1.1
phage
24hr PHYTO1 MOI 1
-0.3
-0.5
-0.1
-1.0
+0.2
agar plate interaction control
Parafilmed volatile agar plate with no fungus
+0.4
-0.2
-0.5
-1.0
-0.3
phage
24hr PHYTO MOI 0.1
-0.6
-0.5
+0.1
-0.6
+0.3
in planta
Rhizosphere from sorghum RTx_430 plants, grown 2 months, low phosphate; Rep 1
-0.1
-0.3
+0.4
-0.6
-0.7
phage
24hr PHYTO6 MOI 1
-0.2
-0.3
-0.9
-0.3
+0.4
phage
24hr PHYTO3 MOI 10
-1.2
+0.4
-0.5
-0.4
+0.4
phage
24hr PHYTO10 MOI 10
-0.5
-0.2
-0.1
-0.8
+0.4
motility
outer cut, LB soft agar motility assay
+0.4
+0.5
-0.5
-0.9
-0.8
nophagecontrol
36hr Phyto Lib only
-0.3
-0.5
+0.1
-0.8
+0.4
phage
36hr PHYTO9 MOI 0.1
-0.3
-0.3
+0.3
-1.0
+0.3
carbon source
Phenylacetic 5 mM (C)
+0.5
-0.3
+0.1
-0.3
-1.1
phage
24hr PHYTO4 MOI 0.1
-0.5
-0.2
-0.3
-0.3
+0.9
phage
36hr PHYTO1 MOI 10
-0.4
-0.3
-0.4
+0.9
+0.4
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