Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS31130 and BPHYT_RS31135 are separated by 287 nucleotidesBPHYT_RS31135 and BPHYT_RS31140 are separated by 46 nucleotidesBPHYT_RS31140 and BPHYT_RS31145 are separated by 220 nucleotidesBPHYT_RS31145 and BPHYT_RS31150 are separated by 208 nucleotides BPHYT_RS31130: BPHYT_RS31130 - hypothetical protein, at 2,540,289 to 2,540,492 _RS31130 BPHYT_RS31135: BPHYT_RS31135 - hexuranate MFS transporter, at 2,540,780 to 2,542,069 _RS31135 BPHYT_RS31140: BPHYT_RS31140 - MFS transporter, at 2,542,116 to 2,543,444 _RS31140 BPHYT_RS31145: BPHYT_RS31145 - glucarate dehydratase, at 2,543,665 to 2,544,939 _RS31145 BPHYT_RS31150: BPHYT_RS31150 - transcriptional regulator, at 2,545,148 to 2,545,852 _RS31150
Group Condition BPHYT_RS31130 BPHYT_RS31135 BPHYT_RS31140 BPHYT_RS31145 BPHYT_RS31150
phage P. phyto PHYTO2 MOI 1 -2.8 +0.3 -0.1 -0.3 -1.4
phage P. phyto PHYTO2 MOI 0.1 -1.1 -0.0 -0.2 +0.1 -2.1
phage PHYTO3 MOI 0.1 -1.2 -0.4 -0.2 -0.5 -0.3
nitrogen source L-Isoleucine (N) -0.5 -0.2 -0.7 -0.2 -1.1
phage 24hr PHYTO10 MOI 1 +0.1 -0.6 -0.5 -0.2 -1.3
phage 36hr PHYTO2 MOI 1 -0.3 -0.9 -0.1 +0.0 -1.1
motility inner cut, LB soft agar motility assay -0.3 -0.1 -0.3 -0.5 -1.2
phage 24hr PHYTO10 MOI 10 -0.6 -0.5 -0.2 -0.1 -0.8
phage 24hr PHYTO2 MOI 1 +0.3 -0.4 -0.6 -0.4 -1.0
phage P. phyto PHYTO2 MOI 1 -0.2 -0.3 +0.1 -0.4 -1.3
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.5X; growth supplemented with 0.4X LB +0.2 -0.5 -0.1 -0.8 -0.6
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -0.9 +0.1 -0.1 +0.1 -0.9
tn7 efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pRH19; outgrowth in LB_gent50 -0.7 -0.1 -0.6 +0.3 -0.5
carbon source Suberic (C) +0.4 -0.1 -0.6 -0.2 -0.9
phage 36hr PHYTO3 MOI 0.1 +0.5 -0.2 -0.4 -0.6 -0.7
phage 24hr PHYTO3 MOI 10 +0.3 -1.2 +0.4 -0.5 -0.4
phage 24hr PHYTO1 MOI 10 +0.4 -0.3 +0.1 -0.3 -1.1
phage 36hr PHYTO9 MOI 0.1 +0.3 -0.3 -0.3 +0.3 -1.0
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant +0.6 -0.1 -0.8 +0.1 -0.9
phage 36hr PHYTO1 MOI 10 -0.8 -0.4 -0.3 -0.4 +0.9
phage 36hr PHYTO8 MOI 0.1 +1.3 -0.5 -0.5 +0.0 -1.3
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.4 -0.0 -0.4 +0.3 -1.0
motility outer cut, LB soft agar motility assay -0.4 +0.4 +0.5 -0.5 -0.9
nophagecontrol 36hr Phyto Lib only +0.9 -0.3 -0.5 +0.1 -0.8
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.9 +0.4 -0.2 -0.5 -1.0
carbon source L-Fucose (C) +0.9 -0.2 +0.1 -0.2 -0.8
phage 36hr PHYTO4 MOI 1 +1.2 -0.7 -0.4 -0.0 -0.1
phage 24hr PHYTO2 MOI 0.1 +0.4 -0.3 +0.7 -0.4 -0.4
nophagecontrol 36hr Phyto Lib only +1.4 -0.7 +0.3 -0.3 -0.3
phage 36hr PHYTO11 MOI 0.1 +1.5 -0.2 +0.3 -0.4 -0.3
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