Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS31060 and BPHYT_RS31065 are separated by 7 nucleotidesBPHYT_RS31065 and BPHYT_RS31070 are separated by 4 nucleotidesBPHYT_RS31070 and BPHYT_RS31075 are separated by 19 nucleotidesBPHYT_RS31075 and BPHYT_RS31080 are separated by 167 nucleotides BPHYT_RS31060: BPHYT_RS31060 - capsular polysaccharide biosynthesis protein, at 2,523,542 to 2,524,822 _RS31060 BPHYT_RS31065: BPHYT_RS31065 - polysaccharide biosynthesis protein, at 2,524,830 to 2,526,194 _RS31065 BPHYT_RS31070: BPHYT_RS31070 - acetyltransferase, at 2,526,199 to 2,526,840 _RS31070 BPHYT_RS31075: BPHYT_RS31075 - hypothetical protein, at 2,526,860 to 2,527,996 _RS31075 BPHYT_RS31080: BPHYT_RS31080 - mannose-1-phosphate guanyltransferase, at 2,528,164 to 2,529,597 _RS31080
Group Condition BPHYT_RS31060 BPHYT_RS31065 BPHYT_RS31070 BPHYT_RS31075 BPHYT_RS31080
in planta Plant=Zea_mays; PlantTreatment=None; sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_R2A_with_Tween_and_Cycloheximide; Time=15_days -2.9 -0.7 N.D. N.D. N.D.
tn7 efficiency Outgrowth after Tn7 transformation -2.1 +0.2 N.D. N.D. N.D.
lb_dap plate control and outgrow on gent LB_DAP agar plate, outgrowth in LB_gent -1.7 -0.3 N.D. N.D. N.D.
phage 24hr PHYTO7 MOI 10 -2.1 +0.3 N.D. N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -1.0 -0.6 N.D. N.D. N.D.
r2a control R2A control -1.0 -0.6 N.D. N.D. N.D.
phage 24hr PHYTO9 MOI 0.1 -1.7 +0.2 N.D. N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -1.3 +0.3 N.D. N.D. N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 1X -1.6 +0.6 N.D. N.D. N.D.
carbon source L-Fucose (C) -1.3 +0.4 N.D. N.D. N.D.
lb LB -1.3 +0.5 N.D. N.D. N.D.
motility outer cut, LB soft agar motility assay -1.5 +0.8 N.D. N.D. N.D.
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB -1.1 +0.6 N.D. N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.9 -0.9 N.D. N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant +1.3 -1.2 N.D. N.D. N.D.
phage 36hr PHYTO7 MOI 1 +1.1 -0.4 N.D. N.D. N.D.
phage 24hr PHYTO7 MOI 1 +1.5 -0.6 N.D. N.D. N.D.
phage 24hr PHYTO1 MOI 10 +1.3 -0.5 N.D. N.D. N.D.
phage 36hr PHYTO6 MOI 10 +1.6 -0.3 N.D. N.D. N.D.
nophagecontrol 24hr Phyto Lib only +1.6 -0.1 N.D. N.D. N.D.
phage 24hr PHYTO3 MOI 10 +1.5 +0.1 N.D. N.D. N.D.
phage 24hr PHYTO2 MOI 0.1 +1.1 +0.7 N.D. N.D. N.D.
phage 24hr PHYTO3 MOI 0.1 +1.3 +0.6 N.D. N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant +1.0 +0.9 N.D. N.D. N.D.
supernatant control Vogels_fungal_media 0.1X +1.1 +1.0 N.D. N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +1.3 +0.8 N.D. N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant -0.1 +2.5 N.D. N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +2.2 +0.3 N.D. N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +2.1 +0.6 N.D. N.D. N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus +2.5 +0.5 N.D. N.D. N.D.
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