Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS30880 and BPHYT_RS30885 are separated by 156 nucleotidesBPHYT_RS30885 and BPHYT_RS30890 are separated by 52 nucleotidesBPHYT_RS30890 and BPHYT_RS30895 are separated by 128 nucleotidesBPHYT_RS30895 and BPHYT_RS30900 are separated by 417 nucleotides BPHYT_RS30880: BPHYT_RS30880 - peptidase S13, at 2,482,140 to 2,483,774 _RS30880 BPHYT_RS30885: BPHYT_RS30885 - hypothetical protein, at 2,483,931 to 2,484,239 _RS30885 BPHYT_RS30890: BPHYT_RS30890 - hypothetical protein, at 2,484,292 to 2,485,062 _RS30890 BPHYT_RS30895: BPHYT_RS30895 - hypothetical protein, at 2,485,191 to 2,485,400 _RS30895 BPHYT_RS30900: BPHYT_RS30900 - transcriptional regulator, at 2,485,818 to 2,486,450 _RS30900
Group Condition BPHYT_RS30880 BPHYT_RS30885 BPHYT_RS30890 BPHYT_RS30895 BPHYT_RS30900
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.5X; growth supplemented with 0.4X LB -0.2 -0.3 -5.1 N.D. +0.5
nitrogen source L-Lysine (N) -0.1 -1.0 -1.6 N.D. -0.6
nitrogen source L-Alanine (N) -1.1 -0.6 -2.1 N.D. +0.5
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.7 -0.4 -1.6 N.D. +0.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant +0.2 -0.3 -2.1 N.D. +0.0
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant +0.2 +0.3 -2.0 N.D. -0.5
phage 36hr PHYTO3 MOI 1 -0.4 -1.3 +0.6 N.D. -0.9
lb_dap plate control LB_DAP agar plate, outgrowth in LB +0.8 -0.7 -1.1 N.D. -0.7
carbon source 2-Deoxy-D-ribonic lithium salt (C) +0.2 -0.3 -2.1 N.D. +0.5
nitrogen source D-Alanine (N) -0.5 -0.3 -1.3 N.D. +0.6
in planta Plant=Brachypodium_distachyon ; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.7 -0.3 -0.9 N.D. +0.6
supernatant control Vogels_fungal_media 0.1X +0.6 -0.3 -1.5 N.D. +0.0
phage 36hr PHYTO8 MOI 10 +0.6 -1.2 -0.2 N.D. -0.4
phage 24hr PHYTO8 MOI 1 +0.7 -1.1 -0.7 N.D. +0.1
replicable vector efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 +0.0 +0.8 -1.2 N.D. -0.6
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB -0.2 +0.4 -1.3 N.D. +0.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant +0.8 +0.4 -0.8 N.D. -0.8
phage 36hr PHYTO4 MOI 1 +0.7 +0.7 -1.2 N.D. -0.4
phage 24hr PHYTO6 MOI 10 +0.1 +0.7 -1.2 N.D. +0.5
phage P. phyto PHYTO2 MOI 0.1 +0.4 -1.2 +0.8 N.D. +0.1
phage 24hr PHYTO3 MOI 1 +0.8 +0.5 -1.2 N.D. +0.0
phage 36hr PHYTO10 MOI 10 +1.0 +0.6 -1.3 N.D. -0.2
carbon source Xylitol (C) +0.6 -0.3 +0.8 N.D. -0.8
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=20h_outgrowth_LB; Time=7_days -0.3 -0.5 +1.3 N.D. +0.4
in planta Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days +0.4 +0.4 -0.7 N.D. +1.0
stress Nickel (II) chloride 1 mM +0.7 -0.1 +1.1 N.D. -0.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant +1.4 -0.1 +1.3 N.D. -0.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.0 -0.2 +1.2 N.D. +1.4
carbon source Phenylacetic 5 mM (C) +0.0 +0.5 +1.0 N.D. +1.0
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.2 +0.4 +6.2 N.D. +0.3
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