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Protein
Homologs
Fitness for 5 genes in
Burkholderia phytofirmans PsJN
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Top 30 experiments (either direction), sorted by average fitness
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500 nt
BPHYT_RS30800 and BPHYT_RS30805 are separated by 145 nucleotides
BPHYT_RS30805 and BPHYT_RS30810 are separated by 373 nucleotides
BPHYT_RS30810 and BPHYT_RS30815 are separated by 113 nucleotides
BPHYT_RS30815 and BPHYT_RS30820 are separated by 213 nucleotides
BPHYT_RS30800: BPHYT_RS30800 - MFS transporter, at 2,465,448 to 2,466,731
_RS30800
BPHYT_RS30805: BPHYT_RS30805 - MFS transporter, at 2,466,877 to 2,468,160
_RS30805
BPHYT_RS30810: BPHYT_RS30810 - transcriptional regulator, at 2,468,534 to 2,469,283
_RS30810
BPHYT_RS30815: BPHYT_RS30815 - 2-methylcitrate dehydratase, at 2,469,397 to 2,470,680
_RS30815
BPHYT_RS30820: BPHYT_RS30820 - GntR family transcriptional regulator, at 2,470,894 to 2,471,634
_RS30820
Group
Condition
BPHYT
_RS30800
BPHYT
_RS30805
BPHYT
_RS30810
BPHYT
_RS30815
BPHYT
_RS30820
in planta
Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days
-2.5
N.D.
-1.7
-0.9
+0.5
phage
36hr PHYTO2 MOI 1
-0.4
N.D.
-3.5
+0.2
-0.2
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
-1.0
N.D.
-0.8
-0.7
-0.3
phage
24hr PHYTO3 MOI 10
-1.8
N.D.
-0.8
-0.3
+0.3
phage
P. phyto PHYTO2 MOI 0.1
-2.0
N.D.
-0.3
-0.2
-0.0
phage
36hr PHYTO1 MOI 1
-1.0
N.D.
-1.3
+0.1
+0.1
agar plate interaction control
Parafilmed volatile agar plate with no fungus
-1.8
N.D.
+0.3
+0.1
-0.6
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant
+0.4
N.D.
-2.0
+0.7
-1.0
phage
24hr PHYTO6 MOI 1
-0.8
N.D.
-1.5
+0.6
-0.0
phage
24hr PHYTO6 MOI 10
+0.4
N.D.
-2.9
+0.7
-0.0
phage
36hr PHYTO7 MOI 1
-1.3
N.D.
-0.4
+0.5
-0.3
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
-0.5
N.D.
-1.4
+0.8
-0.2
replicable vector efficiency
Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50
-1.1
N.D.
-0.8
+0.6
-0.0
in planta
Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-1.4
N.D.
+0.7
-0.0
-0.5
nophagecontrol
24hr Phyto Lib only
-1.6
N.D.
+0.0
-0.2
+0.6
nitrogen source
L-Lysine (N)
+1.0
N.D.
-1.6
+0.5
-1.0
phage
36hr PHYTO6 MOI 10
-1.4
N.D.
+0.8
+0.6
-0.9
nitrogen source
L-Phenylalanine (N)
-1.2
N.D.
-0.0
+0.7
-0.3
phage
24hr PHYTO3 MOI 0.1
-0.2
N.D.
-1.1
-0.2
+0.9
phage
36hr PHYTO10 MOI 10
-1.8
N.D.
+1.4
+0.2
-0.1
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=20h_outgrowth_LB; Time=7_days
-1.7
N.D.
+1.0
+0.5
-0.0
in planta
Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_0.8%agar; Collection=outgrowth_LB; Time=7_days
-1.0
N.D.
+1.2
+0.1
-0.5
in planta
Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth; Time=14_days
+0.1
N.D.
-1.3
+0.9
+0.1
phage
24hr PHYTO2 MOI 0.1
-1.6
N.D.
+1.0
+0.5
+0.1
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant
+3.1
N.D.
-2.6
+0.3
-0.6
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth_LB; Time=7_days
-0.3
N.D.
+1.5
+0.3
-0.6
motility
outer cut, LB soft agar motility assay
-0.6
N.D.
+1.5
+0.6
-0.3
supernatant
Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB
+1.3
N.D.
-1.0
+0.9
+0.5
phage
24hr PHYTO3 MOI 1
+0.9
N.D.
+1.2
+0.3
+0.0
carbon source
Malonic (C)
+2.7
N.D.
+0.3
-0.2
+0.6
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