Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS30745 and BPHYT_RS30750 are separated by 148 nucleotidesBPHYT_RS30750 and BPHYT_RS30755 are separated by 271 nucleotidesBPHYT_RS30755 and BPHYT_RS30760 overlap by 4 nucleotidesBPHYT_RS30760 and BPHYT_RS30765 overlap by 8 nucleotides BPHYT_RS30745: BPHYT_RS30745 - hypothetical protein, at 2,453,574 to 2,453,921 _RS30745 BPHYT_RS30750: BPHYT_RS30750 - GntR family transcriptional regulator, at 2,454,070 to 2,454,762 _RS30750 BPHYT_RS30755: BPHYT_RS30755 - MFS transporter, at 2,455,034 to 2,456,449 _RS30755 BPHYT_RS30760: BPHYT_RS30760 - MmgE/PrpD family protein, at 2,456,446 to 2,457,801 _RS30760 BPHYT_RS30765: BPHYT_RS30765 - hypothetical protein, at 2,457,794 to 2,458,531 _RS30765
Group Condition BPHYT_RS30745 BPHYT_RS30750 BPHYT_RS30755 BPHYT_RS30760 BPHYT_RS30765
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -0.8 -3.9 -0.1 +0.2 N.D.
supernatant control Vogels_fungal_media; pH 5.5 -0.4 -3.3 +0.3 -1.1 N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus -2.4 -2.0 +0.3 -0.2 N.D.
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB +0.6 -5.1 -0.0 +0.3 N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.4 -3.4 -0.4 +0.4 N.D.
nitrogen source L-Glutamic (N) +0.2 -3.2 +0.2 +0.0 N.D.
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -1.3 -2.2 +0.3 +0.5 N.D.
replicable vector efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 +0.3 -2.2 -0.5 -0.3 N.D.
carbon source D-Glucose (C) +0.2 -2.7 +0.3 -0.4 N.D.
carbon source D,L-Lactate (C) -0.1 -2.6 +0.2 +0.0 N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 1X -0.2 -2.7 +0.1 +0.5 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant +0.5 -2.3 +0.1 -0.3 N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.4 -2.4 +0.5 -0.2 N.D.
nophagecontrol 36hr Phyto Lib only -1.2 +0.6 -0.5 -0.6 N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=soil; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.3 -1.5 +0.3 -0.7 N.D.
lb_dap plate control and outgrow on gent LB_DAP agar plate, outgrowth in LB_gent +1.1 -1.7 +0.0 -0.2 N.D.
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.7 -0.9 -0.2 +1.1 N.D.
phage 36hr PHYTO10 MOI 10 -2.1 +1.5 +0.0 +0.1 N.D.
in planta Plant=Zea_mays; PlantTreatment=Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days -0.8 -1.1 +1.0 +0.4 N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus -1.1 -0.9 +0.2 +1.5 N.D.
nophagecontrol 36hr Phyto Lib only -1.4 +1.6 -0.2 -0.3 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant +0.8 -1.5 -0.1 +0.6 N.D.
phage 36hr PHYTO4 MOI 1 -0.7 +1.5 -0.3 -0.6 N.D.
phage 24hr PHYTO6 MOI 10 -1.8 +1.9 -0.0 +0.0 N.D.
phage 24hr PHYTO1 MOI 10 -1.2 +1.5 -0.1 +0.0 N.D.
phage 24hr PHYTO4 MOI 10 -1.7 +1.7 +0.6 +0.0 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant +0.2 +1.2 +0.5 -1.3 N.D.
phage 24hr PHYTO MOI 0.1 -0.4 +2.2 -0.4 -0.7 N.D.
temperature LB 37C -0.1 +2.7 -0.0 +0.7 N.D.
temperature LB 37C +0.1 +4.6 -0.3 +0.2 N.D.
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