Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS30145 and BPHYT_RS30150 are separated by 328 nucleotidesBPHYT_RS30150 and BPHYT_RS30155 are separated by 218 nucleotidesBPHYT_RS30155 and BPHYT_RS30160 overlap by 44 nucleotidesBPHYT_RS30160 and BPHYT_RS30165 overlap by 4 nucleotides BPHYT_RS30145: BPHYT_RS30145 - aconitate hydratase, at 2,315,069 to 2,317,666 _RS30145 BPHYT_RS30150: BPHYT_RS30150 - methylcitrate synthase, at 2,317,995 to 2,319,164 _RS30150 BPHYT_RS30155: BPHYT_RS30155 - Fis family transcriptional regulator, at 2,319,383 to 2,321,374 _RS30155 BPHYT_RS30160: BPHYT_RS30160 - AraC family transcriptional regulator, at 2,321,331 to 2,321,759 _RS30160 BPHYT_RS30165: BPHYT_RS30165 - sarcosine oxidase subunit beta, at 2,321,756 to 2,322,925 _RS30165
Group Condition BPHYT_RS30145 BPHYT_RS30150 BPHYT_RS30155 BPHYT_RS30160 BPHYT_RS30165
carbon source propionate (C) -4.8 -4.8 -3.6 +0.4 +0.1
in planta Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days -0.9 +0.1 -0.4 -1.8 -1.8
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant -1.5 -0.3 -0.6 -0.3 -0.5
agar plate interaction control Parafilmed volatile agar plate with no fungus -1.3 -0.8 -0.0 -0.5 -0.4
nitrogen source L-Lysine (N) -2.0 -0.3 -0.2 -0.1 -0.0
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB -0.5 -0.9 +0.1 -1.0 -0.1
phage 36hr PHYTO3 MOI 1 -1.3 -0.7 +0.1 -0.5 -0.1
phage P. phyto corn MOI 0.1 -1.1 -0.8 +0.1 -0.6 -0.1
phage 36hr PHYTO8 MOI 10 -0.8 -0.2 -0.3 -1.1 +0.1
phage 24hr PHYTO3 MOI 1 -1.5 -0.3 -0.0 -0.9 +0.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant -1.3 -0.2 +0.2 -0.7 -0.3
phage P. phyto corn MOI 1 -0.1 -0.7 -0.2 -1.0 -0.3
carbon source Phenylacetic 5 mM (C) -0.5 -1.1 -0.6 -0.0 -0.1
phage PHYTO3 MOI 0.1 -1.5 -0.3 -0.2 -0.3 +0.0
phage 36hr PHYTO3 MOI 0.1 -0.6 -0.2 +0.0 -1.3 -0.3
phage 36hr PHYTO9 MOI 0.1 -0.2 -0.6 +0.2 -1.1 -0.2
phage 36hr PHYTO8 MOI 10 -1.2 -0.6 -0.3 -0.2 +0.4
replicable vector efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 -1.2 -0.4 +0.0 -0.4 +0.2
in planta Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.5 -0.1 +0.4 -1.1 -0.4
phage 36hr PHYTO6 MOI 0.1 -0.9 -0.9 -0.0 +0.0 +0.3
carbon source 2-Deoxy-D-ribonic lithium salt (C) -1.5 -0.3 -0.1 +0.5 -0.1
phage 24hr PHYTO1 MOI 1 -1.2 -0.4 -0.1 -0.2 +0.4
lb_dap plate control and outgrow on gent LB_DAP agar plate, outgrowth in LB_gent -1.5 +0.4 +0.3 -0.2 -0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -0.7 -0.7 +0.1 +0.5 -0.3
in planta Plant=Zea_mays; PlantTreatment=Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days -0.4 -0.5 -0.2 -0.4 +0.7
phage 36hr PHYTO2 MOI 1 -1.3 -0.3 -0.1 +0.8 +0.2
nophagecontrol 24hr Phyto Lib Only -1.2 -0.3 +0.5 +0.3 +0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant +0.8 -0.8 -0.2 +0.6 -0.2
in planta Plant=Brachypodium_distachyon ; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.3 -0.5 -0.0 +1.5 +1.1
in planta Plant=Brachypodium_distachyon ; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.0 -0.1 -0.2 +1.0 +1.1
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