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Protein
Homologs
Fitness for 5 genes in
Burkholderia phytofirmans PsJN
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Top 30 experiments (either direction), sorted by average fitness
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500 nt
BPHYT_RS30145 and BPHYT_RS30150 are separated by 328 nucleotides
BPHYT_RS30150 and BPHYT_RS30155 are separated by 218 nucleotides
BPHYT_RS30155 and BPHYT_RS30160 overlap by 44 nucleotides
BPHYT_RS30160 and BPHYT_RS30165 overlap by 4 nucleotides
BPHYT_RS30145: BPHYT_RS30145 - aconitate hydratase, at 2,315,069 to 2,317,666
_RS30145
BPHYT_RS30150: BPHYT_RS30150 - methylcitrate synthase, at 2,317,995 to 2,319,164
_RS30150
BPHYT_RS30155: BPHYT_RS30155 - Fis family transcriptional regulator, at 2,319,383 to 2,321,374
_RS30155
BPHYT_RS30160: BPHYT_RS30160 - AraC family transcriptional regulator, at 2,321,331 to 2,321,759
_RS30160
BPHYT_RS30165: BPHYT_RS30165 - sarcosine oxidase subunit beta, at 2,321,756 to 2,322,925
_RS30165
Group
Condition
BPHYT
_RS30145
BPHYT
_RS30150
BPHYT
_RS30155
BPHYT
_RS30160
BPHYT
_RS30165
carbon source
propionate (C)
-4.8
-4.8
-3.6
+0.4
+0.1
in planta
Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days
-0.9
+0.1
-0.4
-1.8
-1.8
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant
-1.5
-0.3
-0.6
-0.3
-0.5
agar plate interaction control
Parafilmed volatile agar plate with no fungus
-1.3
-0.8
-0.0
-0.5
-0.4
nitrogen source
L-Lysine (N)
-2.0
-0.3
-0.2
-0.1
-0.0
supernatant
Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB
-0.5
-0.9
+0.1
-1.0
-0.1
phage
36hr PHYTO3 MOI 1
-1.3
-0.7
+0.1
-0.5
-0.1
phage
P. phyto corn MOI 0.1
-1.1
-0.8
+0.1
-0.6
-0.1
phage
36hr PHYTO8 MOI 10
-0.8
-0.2
-0.3
-1.1
+0.1
phage
24hr PHYTO3 MOI 1
-1.5
-0.3
-0.0
-0.9
+0.4
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant
-1.3
-0.2
+0.2
-0.7
-0.3
phage
P. phyto corn MOI 1
-0.1
-0.7
-0.2
-1.0
-0.3
carbon source
Phenylacetic 5 mM (C)
-0.5
-1.1
-0.6
-0.0
-0.1
phage
PHYTO3 MOI 0.1
-1.5
-0.3
-0.2
-0.3
+0.0
phage
36hr PHYTO3 MOI 0.1
-0.6
-0.2
+0.0
-1.3
-0.3
phage
36hr PHYTO9 MOI 0.1
-0.2
-0.6
+0.2
-1.1
-0.2
phage
36hr PHYTO8 MOI 10
-1.2
-0.6
-0.3
-0.2
+0.4
replicable vector efficiency
Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50
-1.2
-0.4
+0.0
-0.4
+0.2
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days
-0.5
-0.1
+0.4
-1.1
-0.4
phage
36hr PHYTO6 MOI 0.1
-0.9
-0.9
-0.0
+0.0
+0.3
carbon source
2-Deoxy-D-ribonic lithium salt (C)
-1.5
-0.3
-0.1
+0.5
-0.1
phage
24hr PHYTO1 MOI 1
-1.2
-0.4
-0.1
-0.2
+0.4
lb_dap plate control and outgrow on gent
LB_DAP agar plate, outgrowth in LB_gent
-1.5
+0.4
+0.3
-0.2
-0.2
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
-0.7
-0.7
+0.1
+0.5
-0.3
in planta
Plant=Zea_mays; PlantTreatment=Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
-0.4
-0.5
-0.2
-0.4
+0.7
phage
36hr PHYTO2 MOI 1
-1.3
-0.3
-0.1
+0.8
+0.2
nophagecontrol
24hr Phyto Lib Only
-1.2
-0.3
+0.5
+0.3
+0.1
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant
+0.8
-0.8
-0.2
+0.6
-0.2
in planta
Plant=Brachypodium_distachyon ; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days
-0.3
-0.5
-0.0
+1.5
+1.1
in planta
Plant=Brachypodium_distachyon ; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days
+0.0
-0.1
-0.2
+1.0
+1.1
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