Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS30015 and BPHYT_RS30020 are separated by 195 nucleotidesBPHYT_RS30020 and BPHYT_RS30025 are separated by 78 nucleotidesBPHYT_RS30025 and BPHYT_RS30030 are separated by 62 nucleotidesBPHYT_RS30030 and BPHYT_RS30035 are separated by 116 nucleotides BPHYT_RS30015: BPHYT_RS30015 - 2-nitropropane dioxygenase, at 2,289,351 to 2,290,376 _RS30015 BPHYT_RS30020: BPHYT_RS30020 - arsenic transporter, at 2,290,572 to 2,291,843 _RS30020 BPHYT_RS30025: BPHYT_RS30025 - MFS transporter, at 2,291,922 to 2,293,097 _RS30025 BPHYT_RS30030: BPHYT_RS30030 - metallophosphoesterase, at 2,293,160 to 2,294,260 _RS30030 BPHYT_RS30035: BPHYT_RS30035 - hypothetical protein, at 2,294,377 to 2,294,943 _RS30035
Group Condition BPHYT_RS30015 BPHYT_RS30020 BPHYT_RS30025 BPHYT_RS30030 BPHYT_RS30035
phage 36hr PHYTO2 MOI 1 N.D. -1.5 -0.1 -0.1 N.D.
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_0.8%agar; Collection=outgrowth_LB; Time=7_days N.D. -2.2 +0.5 +0.1 N.D.
phage 24hr PHYTO7 MOI 1 N.D. -1.8 +0.1 +0.1 N.D.
phage 36hr PHYTO3 MOI 0.1 N.D. -1.3 +0.1 -0.3 N.D.
replicable vector efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 N.D. -0.7 -0.5 -0.3 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant N.D. -1.2 -0.2 -0.2 N.D.
nitrogen source L-Serine (N) N.D. -0.9 -0.3 -0.3 N.D.
supernatant control Vogels_fungal_media 0.1X N.D. -0.7 -0.2 -0.4 N.D.
phage 36hr PHYTO6 MOI 10 N.D. +0.2 -0.9 -0.5 N.D.
phage 36hr PHYTO8 MOI 0.1 N.D. -0.9 +0.2 -0.3 N.D.
phage 24hr PHYTO3 MOI 10 N.D. +1.2 -0.7 -0.7 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant N.D. -0.9 -0.2 +0.9 N.D.
phage 24hr PHYTO MOI 0.1 N.D. +0.6 -0.4 -0.3 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant N.D. -1.2 +0.2 +1.1 N.D.
phage 24hr PHYTO6 MOI 1 N.D. +0.9 -0.7 +0.1 N.D.
phage 24hr PHYTO11 MOI 1 N.D. +0.9 -0.4 -0.0 N.D.
nophagecontrol 36hr Phyto Lib only N.D. +0.7 +0.6 -0.5 N.D.
temperature LB, 37C N.D. +0.6 +0.5 -0.2 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant N.D. +0.7 -0.2 +0.5 N.D.
in planta Rhizosphere from sorghum RTx_430 plants, grown 2 months, low phosphate; Rep 1 N.D. +0.9 +0.4 -0.2 N.D.
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth; Time=14_days N.D. +0.8 +0.5 -0.1 N.D.
motility outer cut, LB soft agar motility assay N.D. +0.3 +0.6 +0.5 N.D.
nophagecontrol 24hr Phyto Lib Only N.D. +0.8 +0.3 +0.2 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. +1.0 +0.1 +0.2 N.D.
in planta Plant=Arabidopsis_thaliana_FLS2; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_0.8%agar; Collection=outgrowth_LB; Time=7_days N.D. +0.8 +0.7 -0.1 N.D.
phage 36hr PHYTO6 MOI 0.1 N.D. +0.9 +0.5 -0.0 N.D.
phage 24hr PHYTO11 MOI 0.1 N.D. +0.9 +0.0 +0.7 N.D.
phage 24hr PHYTO3 MOI 0.1 N.D. +1.2 +0.4 +0.0 N.D.
motility outer cut, LB soft agar motility assay N.D. +1.3 +0.3 +0.1 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant N.D. +1.3 -0.0 +0.5 N.D.
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