Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS30000 and BPHYT_RS30005 are separated by 89 nucleotidesBPHYT_RS30005 and BPHYT_RS30010 are separated by 80 nucleotidesBPHYT_RS30010 and BPHYT_RS30015 are separated by 531 nucleotidesBPHYT_RS30015 and BPHYT_RS30020 are separated by 195 nucleotides BPHYT_RS30000: BPHYT_RS30000 - hypothetical protein, at 2,285,690 to 2,286,484 _RS30000 BPHYT_RS30005: BPHYT_RS30005 - hypothetical protein, at 2,286,574 to 2,286,834 _RS30005 BPHYT_RS30010: BPHYT_RS30010 - cell division protein, at 2,286,915 to 2,288,819 _RS30010 BPHYT_RS30015: BPHYT_RS30015 - 2-nitropropane dioxygenase, at 2,289,351 to 2,290,376 _RS30015 BPHYT_RS30020: BPHYT_RS30020 - arsenic transporter, at 2,290,572 to 2,291,843 _RS30020
Group Condition BPHYT_RS30000 BPHYT_RS30005 BPHYT_RS30010 BPHYT_RS30015 BPHYT_RS30020
supernatant control Vogels_fungal_media; pH 5.5 +0.0 N.D. -5.9 N.D. -0.5
phage 36hr PHYTO2 MOI 1 -1.4 N.D. -0.9 N.D. -1.5
phage 24hr PHYTO6 MOI 0.1 -0.6 N.D. -2.3 N.D. -0.3
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_0.8%agar; Collection=outgrowth_LB; Time=7_days -0.5 N.D. -0.4 N.D. -2.2
phage 24hr PHYTO4 MOI 10 +0.6 N.D. -3.1 N.D. -0.5
phage 24hr PHYTO7 MOI 1 +0.1 N.D. -1.3 N.D. -1.8
phage 24hr PHYTO1 MOI 10 -0.7 N.D. -1.6 N.D. -0.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant -0.2 N.D. -1.8 N.D. -0.2
in planta Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.3 N.D. -2.3 N.D. -0.3
phage 24hr PHYTO7 MOI 10 -0.5 N.D. -1.3 N.D. -0.3
nitrogen source L-Serine (N) -0.4 N.D. -0.8 N.D. -0.9
nophagecontrol 24hr Phyto Lib Only -0.4 N.D. -1.6 N.D. +0.0
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant +0.2 N.D. -1.0 N.D. -1.2
carbon source Tween 20 (C) +0.2 N.D. -1.0 N.D. -1.0
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant -0.7 N.D. +0.2 N.D. -1.2
phage 36hr PHYTO11 MOI 0.1 +0.2 N.D. -2.2 N.D. +0.3
phage 24hr PHYTO9 MOI 0.1 +0.5 N.D. -2.3 N.D. +0.6
nitrogen source L-Alanine (N) -0.0 N.D. -1.7 N.D. +0.6
carbon source D-Galacturonic Acid (C) +0.5 N.D. -0.8 N.D. -0.8
supernatant control:fungal media R2A with Vogels_fungal_media 0.8X +0.5 N.D. -1.2 N.D. -0.4
nophagecontrol 36hr Phyto Lib only +0.4 N.D. -2.0 N.D. +0.7
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.5X; growth supplemented with 0.4X LB +0.6 N.D. -1.5 N.D. +0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant -0.3 N.D. -1.3 N.D. +1.3
phage 36hr PHYTO1 MOI 10 -0.2 N.D. -1.1 N.D. +1.0
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant -0.4 N.D. +1.1 N.D. -0.9
vitamin With vitamins t1 +0.3 N.D. +1.0 N.D. -0.8
phage 24hr PHYTO11 MOI 0.1 +0.7 N.D. -0.9 N.D. +0.9
phage 24hr PHYTO9 MOI 10 +1.0 N.D. -0.9 N.D. +0.7
motility outer cut, LB soft agar motility assay +0.1 N.D. -0.6 N.D. +1.3
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth_LB; Time=7_days +0.1 N.D. +1.9 N.D. +0.9
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