Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS29755 and BPHYT_RS29765 are separated by 1060 nucleotidesBPHYT_RS29765 and BPHYT_RS29770 are separated by 170 nucleotidesBPHYT_RS29770 and BPHYT_RS29775 are separated by 273 nucleotidesBPHYT_RS29775 and BPHYT_RS29780 are separated by 39 nucleotides BPHYT_RS29755: BPHYT_RS29755 - membrane protein, at 2,229,530 to 2,231,071 _RS29755 BPHYT_RS29765: BPHYT_RS29765 - cell division protein, at 2,232,132 to 2,233,865 _RS29765 BPHYT_RS29770: BPHYT_RS29770 - diguanylate cyclase, at 2,234,036 to 2,235,151 _RS29770 BPHYT_RS29775: BPHYT_RS29775 - hypothetical protein, at 2,235,425 to 2,235,682 _RS29775 BPHYT_RS29780: BPHYT_RS29780 - DSBA oxidoreductase, at 2,235,722 to 2,236,360 _RS29780
Group Condition BPHYT_RS29755 BPHYT_RS29765 BPHYT_RS29770 BPHYT_RS29775 BPHYT_RS29780
in planta Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days -0.8 N.D. +0.3 N.D. -1.0
phage P. phyto PHYTO2 MOI 1 -0.6 N.D. -0.6 N.D. -0.1
phage 24hr PHYTO6 MOI 1 +0.0 N.D. -1.0 N.D. +0.2
replicable vector efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 +0.4 N.D. -0.3 N.D. -0.7
carbon source L-Fucose (C) +0.3 N.D. -0.7 N.D. -0.2
in planta Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.4 N.D. +0.3 N.D. -0.3
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.0 N.D. -0.7 N.D. +0.3
motility inner cut, LB soft agar motility assay -0.0 N.D. -0.6 N.D. +0.3
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.5X; growth supplemented with 0.4X LB +0.4 N.D. -0.4 N.D. -0.2
carbon source Trisodium citrate (C) +0.1 N.D. -0.5 N.D. +0.2
phage 24hr PHYTO2 MOI 1 +0.3 N.D. -0.6 N.D. +0.1
phage 24hr PHYTO6 MOI 10 +0.5 N.D. -0.7 N.D. +0.0
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.2 N.D. -0.3 N.D. +0.5
carbon source adipate (C) +0.0 N.D. -0.3 N.D. +0.5
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.3 N.D. -0.1 N.D. +0.6
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.2 N.D. -0.8 N.D. +1.0
temperature LB, 37C +0.2 N.D. -0.3 N.D. +0.5
phage 36hr PHYTO4 MOI 1 +0.2 N.D. -0.2 N.D. +0.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant -0.2 N.D. +0.1 N.D. +0.6
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.2 N.D. +0.3 N.D. +0.4
in planta Plant=Arabidopsis_thaliana_FLS2; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_0.8%agar; Collection=outgrowth_LB; Time=7_days +0.4 N.D. -0.2 N.D. +0.4
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.3 N.D. -0.3 N.D. +0.8
nophagecontrol 24hr Phyto Lib Only +0.1 N.D. +0.5 N.D. +0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.1 N.D. +0.3 N.D. +0.4
lb_dap plate control and outgrow on gent LB_DAP agar plate, outgrowth in LB_gent +0.1 N.D. +0.7 N.D. +0.0
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.0 N.D. +0.4 N.D. +0.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant +0.2 N.D. +0.1 N.D. +0.6
phage P. phyto PHYTO2 MOI 0.1 +0.4 N.D. +0.4 N.D. +0.2
phage 36hr PHYTO10 MOI 10 +0.6 N.D. +0.2 N.D. +0.3
carbon source Malonic (C) +0.3 N.D. +1.1 N.D. +0.3
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