Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS29665 and BPHYT_RS29670 are separated by 498 nucleotidesBPHYT_RS29670 and BPHYT_RS29675 are separated by 14 nucleotidesBPHYT_RS29675 and BPHYT_RS29680 are separated by 86 nucleotidesBPHYT_RS29680 and BPHYT_RS29685 are separated by 171 nucleotides BPHYT_RS29665: BPHYT_RS29665 - outer membrane insertion signal protein, at 2,208,008 to 2,208,949 _RS29665 BPHYT_RS29670: BPHYT_RS29670 - flagellar motor protein MotA, at 2,209,448 to 2,210,317 _RS29670 BPHYT_RS29675: BPHYT_RS29675 - flagellar motor protein MotB, at 2,210,332 to 2,211,321 _RS29675 BPHYT_RS29680: BPHYT_RS29680 - hypothetical protein, at 2,211,408 to 2,212,091 _RS29680 BPHYT_RS29685: BPHYT_RS29685 - hypothetical protein, at 2,212,263 to 2,212,550 _RS29685
Group Condition BPHYT_RS29665 BPHYT_RS29670 BPHYT_RS29675 BPHYT_RS29680 BPHYT_RS29685
phage 36hr PHYTO8 MOI 10 -0.7 -0.3 -0.2 -0.5 N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 1X +0.0 -0.9 -0.3 -0.3 N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.5X; growth supplemented with 0.4X LB +0.0 -0.9 +0.1 -0.6 N.D.
carbon source 2-Deoxy-D-ribonic lithium salt (C) -0.3 -0.5 -1.0 +0.5 N.D.
phage 24hr PHYTO9 MOI 10 -0.1 +0.5 -0.4 -1.2 N.D.
phage 36hr PHYTO8 MOI 10 -0.6 +0.0 +0.4 -1.0 N.D.
tn7 efficiency Outgrowth after Tn7 transformation +0.3 -0.2 -0.6 -0.5 N.D.
phage 24hr PHYTO11 MOI 0.1 -0.6 +0.5 -0.2 -0.6 N.D.
phage 36hr PHYTO2 MOI 1 -0.2 +0.2 +0.2 -1.1 N.D.
phage P. phyto PHYTO2 MOI 0.1 -0.5 +0.4 -0.3 -0.6 N.D.
phage 36hr PHYTO4 MOI 1 -0.6 +0.4 -0.9 +0.4 N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=20h_outgrowth_LB; Time=7_days -0.6 -0.3 -0.5 +0.8 N.D.
phage 24hr PHYTO3 MOI 10 -1.0 +0.0 +0.3 +0.4 N.D.
lb_dap plate control and outgrow on gent LB_DAP agar plate, outgrowth in LB_gent -0.4 -0.2 +0.8 -0.5 N.D.
lb_dap plate control and outgrow on gent LB_DAP agar plate, outgrowth in LB_gent -0.2 +0.3 +0.5 -0.6 N.D.
in planta Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 +0.7 -0.4 -0.5 +0.1 N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.2 +0.8 -0.4 -0.2 N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB +0.3 -0.4 +0.5 -0.4 N.D.
phage 24hr PHYTO10 MOI 1 -0.9 +0.2 +0.4 +0.5 N.D.
nitrogen source L-Citrulline (N) -0.6 +0.3 +0.8 +0.1 N.D.
replicable vector efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 -0.2 +0.5 +0.8 -0.3 N.D.
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth; Time=14_days +0.8 -0.3 +0.3 +0.2 N.D.
carbon source Phenylacetic 5 mM (C) +0.4 +0.1 +0.9 -0.2 N.D.
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.4 +0.2 +1.0 -0.4 N.D.
carbon source Phenylacetic 5 mM (C) +0.6 -0.2 +0.4 +0.4 N.D.
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_0.8%agar; Collection=outgrowth_LB; Time=7_days +0.6 +0.5 +0.6 -0.1 N.D.
in planta Plant=Arabidopsis_thaliana_FLS2; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_0.8%agar; Collection=outgrowth_LB; Time=7_days +0.5 +0.5 +0.8 -0.2 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant +0.3 -0.2 +1.8 -0.3 N.D.
carbon source D-Glucose 20 mM (C) +0.6 +0.2 +0.4 +0.5 N.D.
carbon source 2-Deoxyadenosine 5-monophosphate 5 mM (C) +0.5 +0.2 +1.3 +0.4 N.D.
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