Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS29430 and BPHYT_RS29435 are separated by 66 nucleotidesBPHYT_RS29435 and BPHYT_RS29440 are separated by 133 nucleotidesBPHYT_RS29440 and BPHYT_RS29445 are separated by 6 nucleotidesBPHYT_RS29445 and BPHYT_RS29450 are separated by 2 nucleotides BPHYT_RS29430: BPHYT_RS29430 - major facilitator transporter, at 2,157,249 to 2,158,550 _RS29430 BPHYT_RS29435: BPHYT_RS29435 - amidohydrolase, at 2,158,617 to 2,159,807 _RS29435 BPHYT_RS29440: BPHYT_RS29440 - LysR family transcriptional regulator, at 2,159,941 to 2,160,849 _RS29440 BPHYT_RS29445: BPHYT_RS29445 - ribose 1,5-bisphosphate phosphokinase, at 2,160,856 to 2,161,410 _RS29445 BPHYT_RS29450: BPHYT_RS29450 - phosphonate metabolism protein, at 2,161,413 to 2,162,135 _RS29450
Group Condition BPHYT_RS29430 BPHYT_RS29435 BPHYT_RS29440 BPHYT_RS29445 BPHYT_RS29450
phage 36hr PHYTO8 MOI 0.1 -0.5 N.D. N.D. N.D. -1.8
phage 36hr PHYTO9 MOI 1 -0.1 N.D. N.D. N.D. -2.1
phage 36hr PHYTO10 MOI 10 -0.2 N.D. N.D. N.D. -1.9
phage P. phyto PHYTO2 MOI 1 +0.1 N.D. N.D. N.D. -2.1
phage 24hr PHYTO3 MOI 10 +0.1 N.D. N.D. N.D. -2.0
carbon source 2-Deoxyadenosine 5-monophosphate 5 mM (C) +0.4 N.D. N.D. N.D. -2.2
supernatant control Vogels_fungal_media; pH 5.5 -0.1 N.D. N.D. N.D. -1.6
phage 36hr PHYTO11 MOI 0.1 -0.1 N.D. N.D. N.D. -1.6
phage 36hr PHYTO11 MOI 1 -0.7 N.D. N.D. N.D. -0.9
phage 36hr PHYTO4 MOI 1 -0.2 N.D. N.D. N.D. -1.5
phage 24hr PHYTO3 MOI 1 -0.0 N.D. N.D. N.D. -1.6
phage 24hr PHYTO6 MOI 0.1 +0.1 N.D. N.D. N.D. -1.7
phage 24hr PHYTO6 MOI 10 -0.3 N.D. N.D. N.D. -1.2
lb_dap plate control and outgrow on gent LB_DAP agar plate, outgrowth in LB_gent +0.3 N.D. N.D. N.D. -1.7
phage 24hr PHYTO1 MOI 1 -0.6 N.D. N.D. N.D. -0.9
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days -0.3 N.D. N.D. N.D. -1.1
temperature LB, 37C -0.6 N.D. N.D. N.D. -0.8
tn7 efficiency Outgrowth after Tn7 transformation -0.2 N.D. N.D. N.D. -1.2
phage 36hr PHYTO6 MOI 10 +0.2 N.D. N.D. N.D. -1.5
phage PHYTO3 MOI 0.1 +0.2 N.D. N.D. N.D. -1.4
phage 24hr PHYTO10 MOI 10 +0.5 N.D. N.D. N.D. -1.6
phage 36hr PHYTO1 MOI 10 +0.4 N.D. N.D. N.D. -1.4
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_0.8%agar; Collection=outgrowth_LB; Time=7_days +0.3 N.D. N.D. N.D. -1.1
in planta Plant=Arabidopsis_thaliana_FLS2; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_0.8%agar; Collection=outgrowth_LB; Time=7_days +0.5 N.D. N.D. N.D. -1.0
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth_LB; Time=7_days -0.5 N.D. N.D. N.D. +1.0
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant +1.5 N.D. N.D. N.D. -0.2
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB +0.5 N.D. N.D. N.D. +1.0
in planta Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days +0.5 N.D. N.D. N.D. +1.1
supernatant control Vogels_fungal_media 0.1X +0.8 N.D. N.D. N.D. +0.7
carbon source Protocatechuic Acid (C) +0.6 N.D. N.D. N.D. +1.1
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