Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS29275 and BPHYT_RS29280 are separated by 153 nucleotidesBPHYT_RS29280 and BPHYT_RS29285 are separated by 190 nucleotidesBPHYT_RS29285 and BPHYT_RS29290 are separated by 2 nucleotidesBPHYT_RS29290 and BPHYT_RS29295 are separated by 171 nucleotides BPHYT_RS29275: BPHYT_RS29275 - serine kinase, at 2,124,088 to 2,125,044 _RS29275 BPHYT_RS29280: BPHYT_RS29280 - 4-aminobutyrate aminotransferase, at 2,125,198 to 2,126,526 _RS29280 BPHYT_RS29285: BPHYT_RS29285 - hypothetical protein, at 2,126,717 to 2,127,451 _RS29285 BPHYT_RS29290: BPHYT_RS29290 - D-amino acid oxidase, at 2,127,454 to 2,128,779 _RS29290 BPHYT_RS29295: BPHYT_RS29295 - FAD-dependent oxidoreductase, at 2,128,951 to 2,130,228 _RS29295
Group Condition BPHYT_RS29275 BPHYT_RS29280 BPHYT_RS29285 BPHYT_RS29290 BPHYT_RS29295
phage 36hr PHYTO8 MOI 10 -0.6 -0.5 N.D. -0.8 -0.9
phage 36hr PHYTO10 MOI 10 -0.4 -0.0 N.D. -1.6 +0.1
phage 36hr PHYTO9 MOI 0.1 +0.1 -0.4 N.D. -0.5 -0.9
phage 24hr PHYTO MOI 0.1 -0.4 -0.4 N.D. -0.4 -0.5
supernatant control Vogels_fungal_media; pH 5.5 -0.6 -0.6 N.D. -0.3 -0.2
phage 36hr PHYTO2 MOI 1 +0.1 -0.7 N.D. -1.1 +0.1
phage P. phyto PHYTO2 MOI 0.1 -0.6 +0.1 N.D. -0.3 -0.7
phage 24hr PHYTO8 MOI 1 -0.1 -0.6 N.D. -0.3 -0.5
phage 36hr PHYTO1 MOI 1 +0.4 -0.5 N.D. -0.5 -0.9
phage 36hr PHYTO10 MOI 10 +0.3 -0.4 N.D. -1.1 -0.1
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB -0.0 -0.6 N.D. +0.1 -0.8
replicable vector efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 +0.1 -0.2 N.D. -0.6 -0.5
in planta Plant=Brachypodium_distachyon ; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -1.1 -0.4 N.D. +0.3 +0.1
phage 36hr PHYTO3 MOI 1 -0.0 +0.4 N.D. -0.8 -0.6
phage PHYTO3 MOI 0.1 +0.4 +0.3 N.D. -1.0 -0.5
phage 36hr PHYTO11 MOI 0.1 -0.2 +0.4 N.D. -0.7 -0.3
phage 24hr PHYTO3 MOI 1 -0.4 +0.1 N.D. -0.9 +0.4
phage 36hr PHYTO6 MOI 1 +0.4 -0.1 N.D. -1.0 +0.0
replicable vector efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 +0.4 -0.1 N.D. -0.5 -0.5
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.8X; growth supplemented with 0.4X LB -0.6 -0.4 N.D. +0.3 +0.4
in planta Rhizosphere from sorghum RTx_430 plants, grown 2 months, low phosphate; Rep 1 +0.5 -0.6 N.D. -0.5 +0.3
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.3 -0.3 N.D. +0.9 -0.6
nophagecontrol 24hr Phyto Lib Only +0.4 +0.2 N.D. -0.6 +0.3
in planta Plant=Zea_mays; PlantTreatment=None; Sample=soil; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.7 +0.3 N.D. -0.4 -0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant -0.1 +0.1 N.D. +1.3 -0.6
in planta Plant=Arabidopsis_thaliana_FLS2; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_0.8%agar; Collection=outgrowth_LB; Time=7_days +0.8 -0.2 N.D. +0.4 -0.1
in planta Plant=Zea_mays; PlantTreatment=None; Sample=soil; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.4 +0.3 N.D. -0.4 +0.7
temperature LB 37C -0.1 +1.8 N.D. -0.2 -0.5
in planta Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.6 +0.3 N.D. -0.1 +0.7
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.5 +0.4 N.D. +0.7 -0.1
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