Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS29125 and BPHYT_RS29130 overlap by 4 nucleotidesBPHYT_RS29130 and BPHYT_RS29135 are separated by 35 nucleotidesBPHYT_RS29135 and BPHYT_RS29140 are separated by 323 nucleotidesBPHYT_RS29140 and BPHYT_RS29145 are separated by 145 nucleotides BPHYT_RS29125: BPHYT_RS29125 - RND transporter, at 2,087,703 to 2,089,199 _RS29125 BPHYT_RS29130: BPHYT_RS29130 - RND transporter MFP subunit, at 2,089,196 to 2,090,419 _RS29130 BPHYT_RS29135: BPHYT_RS29135 - acriflavin resistance protein, at 2,090,455 to 2,093,703 _RS29135 BPHYT_RS29140: BPHYT_RS29140 - glyoxalase, at 2,094,027 to 2,094,461 _RS29140 BPHYT_RS29145: BPHYT_RS29145 - LysR family transcriptional regulator, at 2,094,607 to 2,095,491 _RS29145
Group Condition BPHYT_RS29125 BPHYT_RS29130 BPHYT_RS29135 BPHYT_RS29140 BPHYT_RS29145
phage 36hr PHYTO4 MOI 1 -0.9 +0.2 -1.3 N.D. -0.1
phage 36hr PHYTO1 MOI 10 -0.8 +0.4 -1.3 N.D. -0.4
phage 36hr PHYTO2 MOI 1 -0.2 -0.5 -0.9 N.D. -0.5
phage 24hr PHYTO MOI 0.1 -0.0 -0.3 -0.2 N.D. -1.4
phage 24hr PHYTO4 MOI 10 -0.2 +0.6 -0.8 N.D. -1.2
replicable vector efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 -0.6 -1.0 +0.1 N.D. -0.0
phage P. phyto PHYTO2 MOI 1 -0.3 +0.2 -0.9 N.D. -0.4
phage 24hr PHYTO1 MOI 10 -0.7 +0.2 -0.4 N.D. -0.4
phage 36hr PHYTO11 MOI 1 -0.4 +0.6 -0.2 N.D. -1.1
in planta Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.9 -0.2 +0.3 N.D. -0.3
phage 36hr PHYTO6 MOI 10 -0.2 +0.6 -1.2 N.D. -0.2
phage 36hr PHYTO3 MOI 1 -0.6 +0.6 -0.0 N.D. -0.8
phage P. phyto PHYTO2 MOI 0.1 -0.5 +0.4 -0.7 N.D. +0.0
nophagecontrol 24hr Phyto Lib Only -0.5 +0.6 -0.4 N.D. -0.5
carbon source Suberic (C) -0.2 +0.5 -0.5 N.D. -0.4
in planta Plant=Zea_mays; PlantTreatment=None; Sample=soil; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.4 -0.6 +0.7 N.D. -0.1
phage 24hr PHYTO3 MOI 1 -0.6 +0.6 -0.1 N.D. -0.4
phage 24hr PHYTO10 MOI 1 -0.2 +0.4 +0.3 N.D. -0.8
phage 24hr PHYTO2 MOI 0.1 +0.3 +0.7 -0.6 N.D. -0.6
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.4 +0.5 -0.6 N.D. -0.5
phage 36hr PHYTO2 MOI 1 -0.1 +0.9 -0.6 N.D. -0.3
phage 24hr PHYTO8 MOI 1 +0.6 +0.3 -0.3 N.D. -0.6
phage 36hr PHYTO10 MOI 10 +0.4 +0.5 -0.3 N.D. -0.6
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.2 -0.7 -0.2 N.D. +1.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -0.3 +0.9 +0.3 N.D. -0.3
temperature LB 37C -0.2 +0.1 +1.4 N.D. -0.2
in planta Rhizosphere from sorghum RTx_430 plants, grown 2 months, low phosphate; Rep 1 +0.4 -0.3 +0.8 N.D. +0.4
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days +0.3 +0.8 -0.2 N.D. +0.5
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days +0.2 +0.2 +0.5 N.D. +0.7
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth_LB; Time=7_days +0.6 +0.8 +0.1 N.D. +0.2
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