Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS28750 and BPHYT_RS28755 are separated by 86 nucleotidesBPHYT_RS28755 and BPHYT_RS28760 are separated by 326 nucleotidesBPHYT_RS28760 and BPHYT_RS28765 are separated by 81 nucleotidesBPHYT_RS28765 and BPHYT_RS28770 are separated by 33 nucleotides BPHYT_RS28750: BPHYT_RS28750 - IclR family transcriptional regulator, at 2,003,215 to 2,003,499 _RS28750 BPHYT_RS28755: BPHYT_RS28755 - 3-(2-hydroxyphenyl) propionic acid transporter, at 2,003,586 to 2,005,019 _RS28755 BPHYT_RS28760: BPHYT_RS28760 - porin, at 2,005,346 to 2,006,581 _RS28760 BPHYT_RS28765: BPHYT_RS28765 - fumarylacetoacetate hydrolase, at 2,006,663 to 2,007,847 _RS28765 BPHYT_RS28770: BPHYT_RS28770 - aldehyde dehydrogenase, at 2,007,881 to 2,009,323 _RS28770
Group Condition BPHYT_RS28750 BPHYT_RS28755 BPHYT_RS28760 BPHYT_RS28765 BPHYT_RS28770
phage 36hr PHYTO11 MOI 1 N.D. N.D. +0.1 -0.8 -0.9
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant N.D. N.D. -0.3 -0.3 -0.9
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant N.D. N.D. -0.6 +0.2 -0.7
phage 24hr PHYTO2 MOI 1 N.D. N.D. +0.2 -1.0 -0.2
phage 24hr PHYTO3 MOI 1 N.D. N.D. +0.4 -0.7 -0.8
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. N.D. +0.3 -0.4 -0.8
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth; Time=14_days N.D. N.D. +0.2 -0.6 -0.6
phage 24hr PHYTO10 MOI 1 N.D. N.D. +0.3 -0.2 -0.9
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant N.D. N.D. -0.6 +0.7 -0.8
phage P. phyto corn MOI 0.1 N.D. N.D. +0.3 +0.2 -1.0
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.5X; growth supplemented with 0.4X LB N.D. N.D. -0.4 -0.5 +0.5
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. N.D. +0.6 -0.7 -0.2
phage 36hr PHYTO6 MOI 0.1 N.D. N.D. +0.2 +0.5 -0.8
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant N.D. N.D. +0.4 +0.4 -0.8
carbon source Protocatechuic Acid (C) N.D. N.D. -0.2 +0.8 -0.5
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=20h_outgrowth_LB; Time=7_days N.D. N.D. +0.8 -0.7 +0.0
phage 24hr PHYTO3 MOI 10 N.D. N.D. +0.4 +0.5 -0.6
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth; Time=14_days N.D. N.D. +0.1 +0.9 -0.7
carbon source Azelaic (C) N.D. N.D. -0.2 +0.9 -0.5
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. N.D. +0.4 +0.6 -0.4
in planta Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. N.D. +0.9 -0.5 +0.2
phage 24hr PHYTO4 MOI 10 N.D. N.D. +0.2 +0.8 -0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. N.D. +0.9 -0.2 +0.2
carbon source Deoxyribonucleic from herring sperm 10 mg/mL (C) N.D. N.D. -0.3 +0.6 +0.7
carbon source Protocatechuic Acid (C) N.D. N.D. +0.3 +1.1 -0.5
nophagecontrol 24hr Phyto Lib only N.D. N.D. +0.3 +1.3 -0.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant N.D. N.D. +0.3 +1.2 -0.3
in planta Rhizosphere from sorghum RTx_430 plants, grown 2 months, low phosphate; Rep 1 N.D. N.D. +0.8 -0.0 +0.6
motility outer cut, LB soft agar motility assay N.D. N.D. +0.3 +0.9 +0.3
in planta Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days N.D. N.D. -0.2 +1.8 +0.9
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