Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS28735 and BPHYT_RS28740 are separated by 29 nucleotidesBPHYT_RS28740 and BPHYT_RS28745 are separated by 13 nucleotidesBPHYT_RS28745 and BPHYT_RS28750 are separated by 65 nucleotidesBPHYT_RS28750 and BPHYT_RS28755 are separated by 86 nucleotides BPHYT_RS28735: BPHYT_RS28735 - hypothetical protein, at 2,000,711 to 2,001,406 _RS28735 BPHYT_RS28740: BPHYT_RS28740 - 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase, at 2,001,436 to 2,002,326 _RS28740 BPHYT_RS28745: BPHYT_RS28745 - 2-keto-4-pentenoate hydratase, at 2,002,340 to 2,003,149 _RS28745 BPHYT_RS28750: BPHYT_RS28750 - IclR family transcriptional regulator, at 2,003,215 to 2,003,499 _RS28750 BPHYT_RS28755: BPHYT_RS28755 - 3-(2-hydroxyphenyl) propionic acid transporter, at 2,003,586 to 2,005,019 _RS28755
Group Condition BPHYT_RS28735 BPHYT_RS28740 BPHYT_RS28745 BPHYT_RS28750 BPHYT_RS28755
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant -0.5 -0.5 -1.0 N.D. N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.1 -1.1 -0.7 N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant -0.8 +0.1 -1.2 N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -0.6 -0.4 -0.8 N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant +0.3 -0.9 -1.2 N.D. N.D.
carbon source D-Glucose (C) -0.0 -0.3 -1.3 N.D. N.D.
phage 24hr PHYTO10 MOI 10 -0.7 +0.4 -0.9 N.D. N.D.
supernatant control Vogels_fungal_media; pH 5.5 -0.0 -1.3 +0.2 N.D. N.D.
phage 24hr PHYTO4 MOI 10 -0.3 +0.7 -1.1 N.D. N.D.
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days +0.2 +0.2 -1.1 N.D. N.D.
phage 24hr PHYTO MOI 0.1 -0.3 +0.7 -0.9 N.D. N.D.
nitrogen source L-Glutamic (N) +0.7 -0.2 -0.8 N.D. N.D.
phage 24hr PHYTO6 MOI 10 -0.7 +0.5 +0.2 N.D. N.D.
phage 36hr PHYTO10 MOI 10 +0.6 +0.7 -1.1 N.D. N.D.
vitamin No vitamins t1 +0.4 +0.6 -0.7 N.D. N.D.
phage 24hr PHYTO2 MOI 0.1 -0.7 +0.6 +0.4 N.D. N.D.
phage P. phyto corn MOI 0.1 -0.8 +0.9 +0.3 N.D. N.D.
lb_dap plate control and outgrow on gent LB_DAP agar plate, outgrowth in LB_gent +0.2 +0.8 -0.5 N.D. N.D.
carbon source Phenylacetic 5 mM (C) -0.7 +0.7 +0.5 N.D. N.D.
phage 24hr PHYTO1 MOI 10 -0.5 +0.7 +0.5 N.D. N.D.
phage 36hr PHYTO6 MOI 0.1 -0.4 +0.6 +0.6 N.D. N.D.
phage 24hr PHYTO2 MOI 1 +0.7 +0.4 -0.4 N.D. N.D.
phage P. phyto PHYTO2 MOI 1 +0.0 +1.3 -0.5 N.D. N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.2 +1.4 -0.8 N.D. N.D.
in planta Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days +0.6 -0.3 +0.6 N.D. N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.8X; growth supplemented with 0.4X LB -0.3 +0.6 +0.8 N.D. N.D.
replicable vector efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 +0.8 +0.8 -0.5 N.D. N.D.
phage 36hr PHYTO4 MOI 1 +0.2 +1.0 +0.4 N.D. N.D.
tn7 efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pRH19; outgrowth in LB_gent50 +0.3 +1.4 +0.0 N.D. N.D.
motility outer cut, LB soft agar motility assay -0.1 +0.7 +1.2 N.D. N.D.
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