Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS28480 and BPHYT_RS28485 are separated by 151 nucleotidesBPHYT_RS28485 and BPHYT_RS28490 are separated by 310 nucleotidesBPHYT_RS28490 and BPHYT_RS28495 are separated by 89 nucleotidesBPHYT_RS28495 and BPHYT_RS28500 are separated by 225 nucleotides BPHYT_RS28480: BPHYT_RS28480 - hydroxyglutarate oxidase, at 1,943,720 to 1,944,916 _RS28480 BPHYT_RS28485: BPHYT_RS28485 - GntR family transcriptional regulator, at 1,945,068 to 1,945,769 _RS28485 BPHYT_RS28490: BPHYT_RS28490 - MFS transporter, at 1,946,080 to 1,947,465 _RS28490 BPHYT_RS28495: BPHYT_RS28495 - peptidase M24, at 1,947,555 to 1,948,721 _RS28495 BPHYT_RS28500: BPHYT_RS28500 - LysR family transcriptional regulator, at 1,948,947 to 1,949,903 _RS28500
Group Condition BPHYT_RS28480 BPHYT_RS28485 BPHYT_RS28490 BPHYT_RS28495 BPHYT_RS28500
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.8X; growth supplemented with 0.4X LB +0.3 -5.4 -0.0 -0.1 N.D.
phage 36hr PHYTO1 MOI 10 -3.4 -1.8 -0.0 +0.1 N.D.
replicable vector efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 -0.4 -4.5 +0.0 -0.1 N.D.
tn7 efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pRH19; outgrowth in LB_gent50 -0.2 -4.4 -0.2 -0.2 N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.1 -4.9 +0.4 -0.4 N.D.
phage 36hr PHYTO1 MOI 10 -3.0 -1.8 -0.1 +0.1 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant +0.2 -4.5 -0.2 +0.0 N.D.
supernatant control Vogels_fungal_media; pH 5.5 -1.3 -2.8 -0.3 -0.1 N.D.
phage 36hr PHYTO3 MOI 1 -2.1 -1.9 +0.2 -0.1 N.D.
phage 24hr PHYTO3 MOI 1 -1.9 -1.8 -0.1 +0.1 N.D.
phage P. phyto PHYTO2 MOI 1 -0.6 -2.1 -0.6 -0.3 N.D.
phage PHYTO3 MOI 0.1 -1.6 -2.3 +0.4 +0.1 N.D.
phage 36hr PHYTO1 MOI 1 -1.4 -1.7 -0.1 -0.0 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant -1.5 -1.5 -0.3 +0.0 N.D.
phage 36hr PHYTO6 MOI 10 -1.7 -1.5 +0.2 -0.1 N.D.
phage 36hr PHYTO10 MOI 10 -1.8 -1.5 +0.4 -0.1 N.D.
phage 24hr PHYTO10 MOI 10 -1.3 -1.7 +0.3 -0.3 N.D.
phage 36hr PHYTO1 MOI 1 -1.3 -1.8 +0.1 +0.1 N.D.
phage P. phyto PHYTO2 MOI 1 -0.8 -2.3 +0.4 -0.0 N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.2 -3.0 +0.2 +0.4 N.D.
phage 24hr PHYTO7 MOI 1 -1.2 -2.2 +0.6 +0.2 N.D.
phage 36hr PHYTO2 MOI 1 -1.1 -1.8 +0.4 -0.1 N.D.
phage 24hr PHYTO4 MOI 10 -1.7 -1.1 +0.5 +0.1 N.D.
lb_dap plate control and outgrow on gent LB_DAP agar plate, outgrowth in LB_gent +0.5 -2.8 +0.2 +0.2 N.D.
phage 24hr PHYTO8 MOI 1 +0.6 -2.2 +0.3 -0.5 N.D.
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days +0.8 -2.5 +0.2 +0.2 N.D.
phage 24hr PHYTO3 MOI 0.1 -1.9 +1.4 +0.1 -0.3 N.D.
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days +0.9 -2.0 +0.3 +0.3 N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +2.3 +0.6 -0.1 +0.3 N.D.
in planta Plant=Arabidopsis_thaliana_FLS2; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_0.8%agar; Collection=outgrowth_LB; Time=7_days +0.2 +2.5 +0.3 +0.3 N.D.
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