Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS28375 and BPHYT_RS28380 are separated by 49 nucleotidesBPHYT_RS28380 and BPHYT_RS28385 are separated by 33 nucleotidesBPHYT_RS28385 and BPHYT_RS28390 are separated by 1359 nucleotidesBPHYT_RS28390 and BPHYT_RS28395 are separated by 281 nucleotides BPHYT_RS28375: BPHYT_RS28375 - hypothetical protein, at 1,917,392 to 1,917,952 _RS28375 BPHYT_RS28380: BPHYT_RS28380 - lon protease, at 1,918,002 to 1,920,416 _RS28380 BPHYT_RS28385: BPHYT_RS28385 - molecular chaperone, at 1,920,450 to 1,920,830 _RS28385 BPHYT_RS28390: BPHYT_RS28390 - hypothetical protein, at 1,922,190 to 1,922,459 _RS28390 BPHYT_RS28395: BPHYT_RS28395 - transporter, at 1,922,741 to 1,923,166 _RS28395
Group Condition BPHYT_RS28375 BPHYT_RS28380 BPHYT_RS28385 BPHYT_RS28390 BPHYT_RS28395
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.5X; growth supplemented with 0.4X LB N.D. -1.0 N.D. N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant N.D. -1.0 N.D. N.D. N.D.
lb_dap plate control LB_DAP agar plate, outgrowth in LB N.D. -1.0 N.D. N.D. N.D.
phage 36hr PHYTO6 MOI 1 N.D. -0.9 N.D. N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant N.D. -0.9 N.D. N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant N.D. -0.9 N.D. N.D. N.D.
phage 36hr PHYTO MOI 0.1 N.D. -0.9 N.D. N.D. N.D.
nutrient L-Histidine N.D. -0.9 N.D. N.D. N.D.
phage 36hr PHYTO2 MOI 0.1 N.D. -0.9 N.D. N.D. N.D.
phage 24hr PHYTO4 MOI 10 N.D. -0.8 N.D. N.D. N.D.
carbon source D-Glucose (C) N.D. -0.8 N.D. N.D. N.D.
tn7 efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pRH19; outgrowth in LB_gent50 N.D. -0.8 N.D. N.D. N.D.
carbon source D-Lactate (C) N.D. -0.7 N.D. N.D. N.D.
tn7 efficiency Outgrowth after Tn7 transformation N.D. -0.7 N.D. N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days N.D. -0.7 N.D. N.D. N.D.
supernatant control Vogels_fungal_media; pH 5.5 N.D. -0.7 N.D. N.D. N.D.
phage 36hr PHYTO1 MOI 10 N.D. -0.7 N.D. N.D. N.D.
vitamin With vitamins t2 N.D. -0.7 N.D. N.D. N.D.
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=14_days N.D. -0.7 N.D. N.D. N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.8X; growth supplemented with 0.4X LB N.D. +0.7 N.D. N.D. N.D.
phage 24hr PHYTO2 MOI 0.1 N.D. +0.7 N.D. N.D. N.D.
carbon source Deoxyribonucleic from herring sperm 10 mg/mL (C) N.D. +0.7 N.D. N.D. N.D.
in planta Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days N.D. +0.7 N.D. N.D. N.D.
carbon source Phenylacetic 5 mM (C) N.D. +0.8 N.D. N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. +0.8 N.D. N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant N.D. +0.8 N.D. N.D. N.D.
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_0.8%agar; Collection=outgrowth_LB; Time=7_days N.D. +0.8 N.D. N.D. N.D.
nophagecontrol 24hr Phyto Lib Only N.D. +0.8 N.D. N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. +1.1 N.D. N.D. N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. +1.6 N.D. N.D. N.D.
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