Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS28370 and BPHYT_RS28375 are separated by 506 nucleotidesBPHYT_RS28375 and BPHYT_RS28380 are separated by 49 nucleotidesBPHYT_RS28380 and BPHYT_RS28385 are separated by 33 nucleotidesBPHYT_RS28385 and BPHYT_RS28390 are separated by 1359 nucleotides BPHYT_RS28370: BPHYT_RS28370 - peroxidase, at 1,916,022 to 1,916,885 _RS28370 BPHYT_RS28375: BPHYT_RS28375 - hypothetical protein, at 1,917,392 to 1,917,952 _RS28375 BPHYT_RS28380: BPHYT_RS28380 - lon protease, at 1,918,002 to 1,920,416 _RS28380 BPHYT_RS28385: BPHYT_RS28385 - molecular chaperone, at 1,920,450 to 1,920,830 _RS28385 BPHYT_RS28390: BPHYT_RS28390 - hypothetical protein, at 1,922,190 to 1,922,459 _RS28390
Group Condition BPHYT_RS28370 BPHYT_RS28375 BPHYT_RS28380 BPHYT_RS28385 BPHYT_RS28390
phage 24hr PHYTO1 MOI 10 -3.2 N.D. -0.4 N.D. N.D.
phage 24hr PHYTO11 MOI 1 -2.9 N.D. -0.2 N.D. N.D.
in planta Rhizosphere from sorghum RTx_430 plants, grown 2 months, low phosphate; Rep 1 -2.8 N.D. -0.2 N.D. N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus -1.4 N.D. -0.4 N.D. N.D.
carbon source Malonic (C) -1.4 N.D. -0.4 N.D. N.D.
r2a control R2A control -1.2 N.D. -0.5 N.D. N.D.
phage 24hr PHYTO8 MOI 1 -1.8 N.D. +0.1 N.D. N.D.
phage 36hr PHYTO8 MOI 10 -1.3 N.D. -0.3 N.D. N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus -1.0 N.D. -0.5 N.D. N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus -1.9 N.D. +0.4 N.D. N.D.
phage 36hr PHYTO11 MOI 0.1 -1.2 N.D. -0.2 N.D. N.D.
nitrogen source L-Citrulline (N) -0.9 N.D. -0.6 N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -2.2 N.D. +1.1 N.D. N.D.
nitrogen source L-Serine (N) -1.1 N.D. +0.4 N.D. N.D.
phage 24hr PHYTO9 MOI 0.1 -1.2 N.D. +0.6 N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant +0.6 N.D. -1.0 N.D. N.D.
phage 36hr PHYTO MOI 0.1 +0.6 N.D. -0.9 N.D. N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.5X; growth supplemented with 0.4X LB +0.7 N.D. -1.0 N.D. N.D.
nutrient L-Histidine +0.6 N.D. -0.9 N.D. N.D.
phage 24hr PHYTO4 MOI 10 +0.9 N.D. -0.8 N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.6 N.D. +0.8 N.D. N.D.
vitamin With vitamins t2 +0.9 N.D. -0.7 N.D. N.D.
nophagecontrol 24hr Phyto Lib Only -0.6 N.D. +0.8 N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant +0.9 N.D. -0.5 N.D. N.D.
temperature LB 37C +1.1 N.D. -0.6 N.D. N.D.
carbon source D-Glucose (C) +1.5 N.D. -0.1 N.D. N.D.
carbon source D-Glucose (C) +1.3 N.D. +0.2 N.D. N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.0 N.D. +1.6 N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=soil; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +1.7 N.D. +0.0 N.D. N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.8X; growth supplemented with 0.4X LB +1.0 N.D. +0.7 N.D. N.D.
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BPHYT_RS28375
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BPHYT_RS28390
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