Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS28330 and BPHYT_RS28335 are separated by 134 nucleotidesBPHYT_RS28335 and BPHYT_RS28340 overlap by 20 nucleotidesBPHYT_RS28340 and BPHYT_RS28345 are separated by 49 nucleotidesBPHYT_RS28345 and BPHYT_RS28350 are separated by 152 nucleotides BPHYT_RS28330: BPHYT_RS28330 - transcriptional regulator, at 1,909,348 to 1,909,563 _RS28330 BPHYT_RS28335: BPHYT_RS28335 - hypothetical protein, at 1,909,698 to 1,910,651 _RS28335 BPHYT_RS28340: BPHYT_RS28340 - indole acetimide hydrolase, at 1,910,632 to 1,912,086 _RS28340 BPHYT_RS28345: BPHYT_RS28345 - CDP-diacylglycerol diphosphatase, at 1,912,136 to 1,912,924 _RS28345 BPHYT_RS28350: BPHYT_RS28350 - fatty acid desaturase, at 1,913,077 to 1,914,024 _RS28350
Group Condition BPHYT_RS28330 BPHYT_RS28335 BPHYT_RS28340 BPHYT_RS28345 BPHYT_RS28350
in planta Rhizosphere from sorghum RTx_430 plants, grown 2 months, low phosphate; Rep 1 -0.5 +0.0 +0.1 -1.6 -1.7
replicable vector efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 +0.1 -0.1 +0.2 -0.0 -3.1
in planta Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 -0.1 -0.6 -0.0 -0.4 -1.4
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.7 +0.1 -0.5 -0.7 -0.6
phage 24hr PHYTO11 MOI 0.1 +0.3 -0.6 +0.5 +0.1 -2.4
phage 36hr PHYTO4 MOI 1 +0.4 -0.5 -0.4 +0.2 -1.4
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.0 +0.1 -0.6 +0.4 -1.6
in planta Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days -1.4 -0.2 -0.5 -0.1 +0.6
in planta Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.6 +0.6 -0.2 -0.6 -0.6
carbon source 2-Deoxyadenosine 5-monophosphate 5 mM (C) +0.3 +0.4 -0.1 -0.3 -1.6
in planta Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -1.0 +0.4 -0.2 -1.1 +0.6
phage 24hr PHYTO6 MOI 1 +0.7 +0.0 -0.5 +0.2 -1.7
carbon source D-Glucose (C) -0.0 -0.1 +0.6 +0.2 -1.5
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.5X; growth supplemented with 0.4X LB +0.3 +0.1 +0.6 -0.3 -1.6
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant +0.5 +0.1 +0.7 -0.1 -1.9
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant +0.8 +0.3 +0.3 +0.3 -2.2
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.7 +0.2 -0.4 +0.3 -1.4
lb_dap plate control and outgrow on gent LB_DAP agar plate, outgrowth in LB_gent +0.4 +0.2 +0.6 -0.1 -1.2
phage 36hr PHYTO10 MOI 10 +1.0 +0.3 +0.4 +0.2 -1.2
phage 36hr PHYTO6 MOI 10 +0.2 -0.3 -0.3 +0.6 +1.2
carbon source Malonic (C) +0.8 -0.0 +0.1 -0.4 +1.3
nitrogen source L-Asparagine (N) -0.3 +0.1 +0.1 +0.5 +1.4
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days +0.3 +0.2 +1.0 -0.2 +0.6
phage 24hr PHYTO MOI 0.1 -0.1 -0.1 +0.3 +0.2 +1.8
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.1 -0.1 +0.8 +0.0 +1.5
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.9 -0.2 +0.0 +0.8 +0.7
phage 36hr PHYTO MOI 0.1 +0.6 -0.0 +0.3 +0.6 +0.8
phage 24hr PHYTO3 MOI 1 +0.7 +0.1 +0.5 +0.3 +0.9
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.8 -0.0 +0.5 +0.4 +1.2
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.4 +0.3 +2.0 +0.5 +0.9
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