Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS28035 and BPHYT_RS28040 are separated by 111 nucleotidesBPHYT_RS28040 and BPHYT_RS28045 are separated by 270 nucleotidesBPHYT_RS28045 and BPHYT_RS28050 are separated by 84 nucleotidesBPHYT_RS28050 and BPHYT_RS28055 overlap by 4 nucleotides BPHYT_RS28035: BPHYT_RS28035 - AMP-dependent synthetase, at 1,852,974 to 1,854,665 _RS28035 BPHYT_RS28040: BPHYT_RS28040 - acyl-CoA dehydrogenase, at 1,854,777 to 1,855,910 _RS28040 BPHYT_RS28045: BPHYT_RS28045 - AraC family transcriptional regulator, at 1,856,181 to 1,857,218 _RS28045 BPHYT_RS28050: BPHYT_RS28050 - serine protease, at 1,857,303 to 1,858,916 _RS28050 BPHYT_RS28055: BPHYT_RS28055 - membrane protein, at 1,858,913 to 1,859,686 _RS28055
Group Condition BPHYT_RS28035 BPHYT_RS28040 BPHYT_RS28045 BPHYT_RS28050 BPHYT_RS28055
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.8X; growth supplemented with 0.4X LB N.D. -2.0 -0.6 -0.3 -0.6
carbon source Tween 20 (C) N.D. -1.4 +0.2 -0.7 -1.6
supernatant control Vogels_fungal_media; pH 5.5 N.D. -1.1 +0.0 -0.4 -1.5
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. -1.1 -0.2 +0.1 -1.6
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.5X; growth supplemented with 0.4X LB N.D. -0.7 -0.4 -0.7 -0.5
phage 24hr PHYTO MOI 0.1 N.D. -0.3 +0.1 +0.2 -2.2
supernatant control Vogels_fungal_media 0.1X N.D. +0.2 -0.6 -0.5 -1.1
motility inner cut, LB soft agar motility assay N.D. -0.7 -0.2 -0.1 -1.1
phage 24hr PHYTO MOI 0.1 N.D. -0.9 -0.6 +0.4 -0.9
phage 24hr PHYTO4 MOI 0.1 N.D. -0.2 -0.4 +0.3 -1.5
carbon source Malonic (C) N.D. +0.1 -0.3 -0.1 -1.5
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. -1.1 -0.1 +0.2 -0.8
phage 36hr PHYTO7 MOI 1 N.D. +0.5 -0.2 +0.0 -2.1
phage 36hr PHYTO3 MOI 1 N.D. +0.3 +0.2 -0.1 -1.9
in planta Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days N.D. -0.1 +0.6 -0.6 -1.1
nitrogen source Uridine (N) N.D. -0.3 -0.6 +0.4 -0.6
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant N.D. -1.3 -0.3 +0.2 +0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant N.D. -0.9 -0.6 -0.0 +0.5
phage 36hr PHYTO6 MOI 10 N.D. +0.1 -0.1 +0.4 -1.4
lb_dap plate control and outgrow on gent LB_DAP agar plate, outgrowth in LB_gent N.D. -1.2 +0.7 +0.1 -0.3
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. +0.9 -0.3 -0.4 -0.9
phage 24hr PHYTO10 MOI 10 N.D. -0.4 -0.6 +0.2 +0.7
carbon source 2-Deoxyadenosine 5-monophosphate 5 mM (C) N.D. -0.4 -0.3 -0.3 +1.1
lb_dap plate control and outgrow on gent LB_DAP agar plate, outgrowth in LB_gent N.D. -0.4 +0.5 +0.6 -0.5
phage 36hr PHYTO3 MOI 0.1 N.D. +0.5 +0.2 +0.5 -0.9
phage P. phyto PHYTO2 MOI 0.1 N.D. +0.5 -0.2 -0.4 +1.0
in planta Plant=Zea_mays; PlantTreatment=None; Sample=soil; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. -0.3 +0.7 +0.4 +0.9
in planta Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 N.D. +0.1 -0.3 +0.0 +1.8
in planta Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. +0.5 +0.2 +1.0 +0.5
in planta Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. +0.6 +0.9 +0.9 +0.0
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