Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS28025 and BPHYT_RS28030 are separated by 24 nucleotidesBPHYT_RS28030 and BPHYT_RS28035 are separated by 72 nucleotidesBPHYT_RS28035 and BPHYT_RS28040 are separated by 111 nucleotidesBPHYT_RS28040 and BPHYT_RS28045 are separated by 270 nucleotides BPHYT_RS28025: BPHYT_RS28025 - 3-hydroxyisobutyrate dehydrogenase, at 1,850,451 to 1,851,365 _RS28025 BPHYT_RS28030: BPHYT_RS28030 - methylmalonate-semialdehyde dehydrogenase, at 1,851,390 to 1,852,901 _RS28030 BPHYT_RS28035: BPHYT_RS28035 - AMP-dependent synthetase, at 1,852,974 to 1,854,665 _RS28035 BPHYT_RS28040: BPHYT_RS28040 - acyl-CoA dehydrogenase, at 1,854,777 to 1,855,910 _RS28040 BPHYT_RS28045: BPHYT_RS28045 - AraC family transcriptional regulator, at 1,856,181 to 1,857,218 _RS28045
Group Condition BPHYT_RS28025 BPHYT_RS28030 BPHYT_RS28035 BPHYT_RS28040 BPHYT_RS28045
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.8X; growth supplemented with 0.4X LB -1.0 -1.4 N.D. -2.0 -0.6
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.5X; growth supplemented with 0.4X LB -4.2 -0.4 N.D. -0.4 +0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant -1.5 -0.0 N.D. -1.3 -0.3
nitrogen source L-Alanine (N) -1.0 -1.2 N.D. +0.2 -0.6
temperature LB, 23C -1.5 -0.3 N.D. -0.5 -0.4
phage P. phyto PHYTO2 MOI 1 -3.1 +0.1 N.D. +0.2 +0.1
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.5 -1.3 N.D. -0.4 -0.4
phage 24hr PHYTO4 MOI 0.1 -1.9 +0.2 N.D. -0.2 -0.4
in planta Rhizosphere from sorghum RTx_430 plants, grown 2 months, low phosphate; Rep 1 +0.7 -1.8 N.D. -1.2 +0.1
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=14_days -1.8 +0.6 N.D. -0.7 -0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -1.5 +0.6 N.D. -0.8 +0.1
lb_dap plate control and outgrow on gent LB_DAP agar plate, outgrowth in LB_gent -2.2 +0.6 N.D. -0.4 +0.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -1.8 +0.6 N.D. -0.0 -0.2
phage 24hr PHYTO10 MOI 10 +0.8 -1.2 N.D. -0.4 -0.6
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -1.4 +0.4 N.D. -0.6 +0.4
phage 24hr PHYTO3 MOI 10 -1.6 -0.5 N.D. +0.9 -0.0
in planta Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.7 -1.2 N.D. +0.5 +0.2
in planta Plant=Zea_mays; PlantTreatment=None; Sample=soil; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -1.7 -0.4 N.D. +0.5 +0.6
in planta Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -2.5 +0.3 N.D. +0.6 +0.9
nophagecontrol 24hr Phyto Lib Only -1.5 +0.6 N.D. +0.4 +0.2
phage 24hr PHYTO9 MOI 10 -1.0 +0.9 N.D. -0.4 +0.3
carbon source D-Glucose 20 mM (C) +1.3 -0.4 N.D. -0.5 -0.4
carbon source Tween 20 (C) +1.1 +0.1 N.D. -1.4 +0.2
phage 24hr PHYTO2 MOI 1 -0.6 +0.9 N.D. +0.4 -0.6
lb_dap plate control and outgrow on gent LB_DAP agar plate, outgrowth in LB_gent +0.3 +0.5 N.D. -1.2 +0.7
nitrogen source Ammonium chloride (N) -0.8 +1.1 N.D. +0.4 -0.4
phage P. phyto PHYTO2 MOI 1 +1.0 +0.6 N.D. -0.4 -0.6
lb_dap plate control LB_DAP agar plate, outgrowth in LB +1.8 -0.5 N.D. -0.4 -0.1
carbon source 2-Deoxyadenosine 5-monophosphate 5 mM (C) +1.4 +0.6 N.D. -0.4 -0.3
phage 24hr PHYTO3 MOI 1 +1.5 +0.6 N.D. +0.5 +0.4
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BPHYT_RS28025
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BPHYT_RS28030
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BPHYT_RS28045
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