Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS27930 and BPHYT_RS27935 are separated by 1683 nucleotidesBPHYT_RS27935 and BPHYT_RS27940 are separated by 102 nucleotidesBPHYT_RS27940 and BPHYT_RS27945 are separated by 302 nucleotidesBPHYT_RS27945 and BPHYT_RS27950 are separated by 14 nucleotides BPHYT_RS27930: BPHYT_RS27930 - LysR family transcriptional regulator, at 1,830,835 to 1,831,788 _RS27930 BPHYT_RS27935: BPHYT_RS27935 - PadR family transcriptional regulator, at 1,833,472 to 1,834,335 _RS27935 BPHYT_RS27940: BPHYT_RS27940 - FAD-binding protein, at 1,834,438 to 1,835,289 _RS27940 BPHYT_RS27945: BPHYT_RS27945 - dihydroxyacetone kinase, at 1,835,592 to 1,836,581 _RS27945 BPHYT_RS27950: BPHYT_RS27950 - dihydroxyacetone kinase, at 1,836,596 to 1,837,228 _RS27950
Group Condition BPHYT_RS27930 BPHYT_RS27935 BPHYT_RS27940 BPHYT_RS27945 BPHYT_RS27950
phage 24hr PHYTO9 MOI 10 N.D. N.D. -1.8 N.D. -0.3
phage 36hr PHYTO8 MOI 10 N.D. N.D. -0.6 N.D. -0.9
vitamin No vitamins t2 N.D. N.D. -1.1 N.D. -0.4
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. N.D. -0.9 N.D. -0.4
supernatant control:fungal media R2A with Vogels_fungal_media 0.8X N.D. N.D. -1.0 N.D. -0.3
nitrogen source L-Serine (N) N.D. N.D. -0.7 N.D. -0.6
phage 24hr PHYTO11 MOI 0.1 N.D. N.D. -0.5 N.D. -0.7
in planta Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days N.D. N.D. +0.5 N.D. -1.5
carbon source 2-Deoxy-D-ribonic lithium salt (C) N.D. N.D. +0.2 N.D. -1.0
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB N.D. N.D. +0.4 N.D. -1.2
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.8X; growth supplemented with 0.4X LB N.D. N.D. -0.9 N.D. +0.3
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. N.D. +0.3 N.D. -0.9
phage 36hr PHYTO7 MOI 0.1 N.D. N.D. +0.4 N.D. -1.0
in planta Rhizosphere from sorghum RTx_430 plants, grown 2 months, low phosphate; Rep 1 N.D. N.D. +1.1 N.D. -1.3
phage 24hr PHYTO6 MOI 0.1 N.D. N.D. +0.8 N.D. -0.9
phage 24hr PHYTO3 MOI 10 N.D. N.D. +0.7 N.D. -0.8
phage 36hr PHYTO3 MOI 1 N.D. N.D. +0.6 N.D. -0.6
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant N.D. N.D. -0.6 N.D. +0.6
phage 36hr PHYTO11 MOI 1 N.D. N.D. +0.7 N.D. -0.6
phage 36hr PHYTO9 MOI 1 N.D. N.D. +1.0 N.D. -0.8
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 1X N.D. N.D. -0.5 N.D. +0.9
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 1X N.D. N.D. -0.4 N.D. +0.8
phage 36hr PHYTO11 MOI 0.1 N.D. N.D. +0.9 N.D. -0.4
pH LB, pH4 N.D. N.D. +1.0 N.D. -0.3
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. N.D. +1.2 N.D. +0.1
phage 24hr PHYTO10 MOI 1 N.D. N.D. +0.7 N.D. +0.5
phage 36hr PHYTO8 MOI 0.1 N.D. N.D. +1.1 N.D. +0.3
phage 36hr PHYTO6 MOI 1 N.D. N.D. +0.9 N.D. +0.6
temperature LB, 37C N.D. N.D. +1.1 N.D. +0.8
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. N.D. +1.3 N.D. +0.7
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