Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS27875 and BPHYT_RS27880 are separated by 565 nucleotidesBPHYT_RS27880 and BPHYT_RS27890 are separated by 820 nucleotidesBPHYT_RS27890 and BPHYT_RS27895 are separated by 42 nucleotidesBPHYT_RS27895 and BPHYT_RS27900 are separated by 172 nucleotides BPHYT_RS27875: BPHYT_RS27875 - CreA family protein, at 1,817,219 to 1,817,686 _RS27875 BPHYT_RS27880: BPHYT_RS27880 - diguanylate phosphodiesterase, at 1,818,252 to 1,821,431 _RS27880 BPHYT_RS27890: BPHYT_RS27890 - hypothetical protein, at 1,822,252 to 1,822,512 _RS27890 BPHYT_RS27895: BPHYT_RS27895 - enoyl-CoA hydratase, at 1,822,555 to 1,822,998 _RS27895 BPHYT_RS27900: BPHYT_RS27900 - 2-hydroxyacid dehydrogenase, at 1,823,171 to 1,824,160 _RS27900
Group Condition BPHYT_RS27875 BPHYT_RS27880 BPHYT_RS27890 BPHYT_RS27895 BPHYT_RS27900
replicable vector efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 -0.1 -0.0 -3.7 -0.2 N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=soil; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -1.3 -0.4 -1.5 -0.4 N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.5 -0.5 -0.7 -1.6 N.D.
phage 24hr PHYTO9 MOI 0.1 +0.6 -0.1 -2.9 -0.4 N.D.
phage P. phyto PHYTO2 MOI 1 +0.1 -0.0 -3.5 +1.0 N.D.
carbon source adipate (C) -0.1 +0.3 -2.4 +0.5 N.D.
phage 36hr PHYTO10 MOI 1 -2.4 -0.1 +1.2 +0.0 N.D.
phage PHYTO3 MOI 0.1 -0.3 +0.1 -1.7 +0.9 N.D.
phage 24hr PHYTO4 MOI 10 -0.1 -0.4 +1.5 -1.9 N.D.
carbon source Malonic (C) -0.3 -0.1 -2.6 +2.4 N.D.
nophagecontrol 24hr Phyto Lib Only -0.4 +0.1 +1.7 -1.8 N.D.
carbon source Disodium Glutarate (C) +0.4 -0.1 -1.4 +1.4 N.D.
phage 36hr PHYTO8 MOI 0.1 -1.5 -0.2 +1.7 +0.3 N.D.
phage 36hr PHYTO2 MOI 1 -2.1 -0.1 +2.6 -0.1 N.D.
in planta Plant=Arabidopsis_thaliana_FLS2; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_0.8%agar; Collection=outgrowth_LB; Time=7_days -0.0 +0.4 -1.4 +1.5 N.D.
phage 24hr PHYTO2 MOI 0.1 -0.2 +0.3 +2.3 -1.8 N.D.
phage 36hr PHYTO6 MOI 0.1 -0.9 -0.2 +2.0 +0.1 N.D.
nophagecontrol 24hr Phyto Lib Only +0.6 -0.6 +2.0 -0.4 N.D.
phage 36hr PHYTO MOI 0.1 -0.9 +0.1 +2.2 +0.4 N.D.
phage 36hr PHYTO8 MOI 10 -0.6 -0.0 +2.0 +0.5 N.D.
phage 36hr PHYTO6 MOI 0.1 -0.6 -0.1 +2.5 +0.1 N.D.
phage 24hr PHYTO3 MOI 10 -0.5 -0.3 +1.9 +1.0 N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB +0.6 -0.4 +1.7 +0.3 N.D.
phage 24hr PHYTO3 MOI 0.1 +0.2 -0.0 +2.7 -0.5 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant -0.3 +0.0 +1.0 +2.0 N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.5X; growth supplemented with 0.4X LB -0.0 +0.0 +2.0 +1.0 N.D.
phage 24hr PHYTO6 MOI 10 +0.1 -0.2 +3.4 -0.0 N.D.
phage 24hr PHYTO6 MOI 0.1 +0.0 -0.1 +2.9 +0.5 N.D.
phage 24hr PHYTO1 MOI 10 -0.7 +0.1 +3.5 +0.4 N.D.
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=14_days +0.1 +0.1 +1.9 +1.5 N.D.
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BPHYT_RS27880
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