Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS27785 and BPHYT_RS27790 are separated by 85 nucleotidesBPHYT_RS27790 and BPHYT_RS27795 are separated by 398 nucleotidesBPHYT_RS27795 and BPHYT_RS27800 are separated by 187 nucleotidesBPHYT_RS27800 and BPHYT_RS27805 are separated by 79 nucleotides BPHYT_RS27785: BPHYT_RS27785 - hypothetical protein, at 1,794,767 to 1,795,372 _RS27785 BPHYT_RS27790: BPHYT_RS27790 - poly-beta-hydroxybutyrate polymerase, at 1,795,458 to 1,797,314 _RS27790 BPHYT_RS27795: BPHYT_RS27795 - nitroreductase, at 1,797,713 to 1,798,381 _RS27795 BPHYT_RS27800: BPHYT_RS27800 - esterase, at 1,798,569 to 1,799,834 _RS27800 BPHYT_RS27805: BPHYT_RS27805 - RNA-metabolising metallo-beta-lactamase, at 1,799,914 to 1,801,272 _RS27805
Group Condition BPHYT_RS27785 BPHYT_RS27790 BPHYT_RS27795 BPHYT_RS27800 BPHYT_RS27805
phage 36hr PHYTO3 MOI 1 -0.0 -0.4 +0.0 -2.6 -0.4
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth; Time=14_days -0.8 +0.1 -0.6 -0.9 -0.7
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth_LB; Time=7_days -0.2 -0.4 -0.3 -0.8 -1.2
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth_LB; Time=7_days -0.1 -0.3 -0.3 -0.6 -1.1
phage 24hr PHYTO11 MOI 0.1 +0.3 +0.0 -0.6 -1.5 -0.6
replicable vector efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 -1.7 +0.4 -0.2 -0.4 -0.5
phage 36hr PHYTO4 MOI 1 +0.6 +0.0 -0.3 -1.3 -0.8
phage P. phyto PHYTO2 MOI 1 +1.0 -0.1 -0.4 -1.7 -0.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant -1.2 +0.2 -0.3 -0.6 +0.4
phage 24hr PHYTO9 MOI 10 -0.1 -0.4 -0.7 -1.0 +0.8
phage 36hr PHYTO8 MOI 10 +0.6 -0.0 -0.4 -0.2 -1.1
nitrogen source D-Alanine (N) +0.9 -0.4 -0.3 -1.0 -0.1
carbon source Azelaic (C) +0.3 -0.3 -0.5 -0.9 +0.6
in planta Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 +0.0 -0.9 -0.6 +0.9 -0.1
in planta Plant=Arabidopsis_thaliana_FLS2; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_0.8%agar; Collection=outgrowth_LB; Time=7_days -1.3 +0.6 +0.0 -0.1 +0.4
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.1 -0.1 -0.0 -1.2 +0.9
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant -0.0 +0.7 -0.4 -1.0 +0.5
supernatant control Vogels_fungal_media; pH 5.5 -0.8 -0.4 +0.1 +1.0 +0.2
carbon source Protocatechuic Acid (C) +0.4 -0.4 -0.1 -0.6 +1.2
in planta Plant=Zea_mays; PlantTreatment=None; Sample=soil; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.6 +1.2 +0.4 -1.2 +0.8
in planta Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.0 +0.6 +0.5 -0.9 +0.6
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB -0.4 -0.0 -0.4 -0.1 +1.7
carbon source Disodium Glutarate (C) +1.2 -0.5 -0.0 -0.3 +0.6
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -0.4 +0.4 +0.2 -0.3 +1.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +1.5 -0.3 +0.5 -0.6 +0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.3 -0.5 +0.3 +0.9 +0.6
in planta Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.4 +1.2 +0.4 -0.4 +0.5
in planta Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.8 +1.2 +0.5 -0.7 +0.6
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.3 +0.3 +0.0 +0.4 +1.3
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +1.0 +0.9 +0.5 +0.0 +0.5
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