Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS27720 and BPHYT_RS27725 are separated by 0 nucleotidesBPHYT_RS27725 and BPHYT_RS27730 are separated by 46 nucleotidesBPHYT_RS27730 and BPHYT_RS27735 are separated by 171 nucleotidesBPHYT_RS27735 and BPHYT_RS27740 are separated by 37 nucleotides BPHYT_RS27720: BPHYT_RS27720 - hypothetical protein, at 1,783,592 to 1,783,831 _RS27720 BPHYT_RS27725: BPHYT_RS27725 - hypothetical protein, at 1,783,832 to 1,784,050 _RS27725 BPHYT_RS27730: BPHYT_RS27730 - LuxR family transcriptional regulator, at 1,784,097 to 1,784,744 _RS27730 BPHYT_RS27735: BPHYT_RS27735 - nitroreductase, at 1,784,916 to 1,785,947 _RS27735 BPHYT_RS27740: BPHYT_RS27740 - hypothetical protein, at 1,785,985 to 1,786,260 _RS27740
Group Condition BPHYT_RS27720 BPHYT_RS27725 BPHYT_RS27730 BPHYT_RS27735 BPHYT_RS27740
agar plate interaction control Parafilmed volatile agar plate with no fungus -2.8 N.D. N.D. -0.0 N.D.
phage 24hr PHYTO9 MOI 10 -3.1 N.D. N.D. +0.5 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant -3.0 N.D. N.D. +0.5 N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.8X; growth supplemented with 0.4X LB -1.6 N.D. N.D. -0.9 N.D.
supernatant control Vogels_fungal_media 0.1X -1.4 N.D. N.D. -0.5 N.D.
phage 24hr PHYTO1 MOI 1 -2.0 N.D. N.D. +0.0 N.D.
carbon source Malonic (C) -2.2 N.D. N.D. +0.3 N.D.
supernatant control Vogels_fungal_media 0.1X -1.1 N.D. N.D. -0.7 N.D.
phage P. phyto corn MOI 1 -1.7 N.D. N.D. +0.1 N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -1.3 N.D. N.D. +0.1 N.D.
nophagecontrol 36hr Phyto Lib Only -1.1 N.D. N.D. +0.4 N.D.
nitrogen source L-Lysine (N) -1.6 N.D. N.D. +0.9 N.D.
phage 24hr PHYTO3 MOI 0.1 -1.2 N.D. N.D. +0.5 N.D.
nitrogen source L-Phenylalanine (N) -1.1 N.D. N.D. +0.5 N.D.
phage 24hr PHYTO3 MOI 10 -1.3 N.D. N.D. +0.7 N.D.
supernatant control:fungal media R2A with Vogels_fungal_media 0.2X -1.3 N.D. N.D. +0.7 N.D.
phage 36hr PHYTO3 MOI 1 -1.0 N.D. N.D. +0.8 N.D.
nophagecontrol 24hr Phyto Lib Only -0.7 N.D. N.D. +0.8 N.D.
replicable vector efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 +0.9 N.D. N.D. -0.9 N.D.
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth; Time=14_days -0.6 N.D. N.D. +0.9 N.D.
in planta Plant=Brachypodium_distachyon ; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +1.1 N.D. N.D. -0.3 N.D.
nitrogen source L-Glutamic (N) +1.5 N.D. N.D. -0.1 N.D.
in planta Plant=Zea_mays; PlantTreatment=Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days +1.6 N.D. N.D. -0.2 N.D.
supernatant control:fungal media R2A with Vogels_fungal_media 0.8X +0.9 N.D. N.D. +0.6 N.D.
nophagecontrol 24hr Phyto Lib only +1.0 N.D. N.D. +0.6 N.D.
phage P. phyto PHYTO2 MOI 1 +0.6 N.D. N.D. +0.9 N.D.
nitrogen source L-Glutamine (N) +1.1 N.D. N.D. +0.5 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +1.4 N.D. N.D. +0.2 N.D.
nitrogen source L-Proline (N) +1.2 N.D. N.D. +0.4 N.D.
nitrogen source L-Isoleucine (N) +1.2 N.D. N.D. +0.5 N.D.
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