Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS27625 and BPHYT_RS27635 are separated by 604 nucleotidesBPHYT_RS27635 and BPHYT_RS27640 are separated by 431 nucleotidesBPHYT_RS27640 and BPHYT_RS27645 are separated by 467 nucleotidesBPHYT_RS27645 and BPHYT_RS27650 are separated by 58 nucleotides BPHYT_RS27625: BPHYT_RS27625 - transport-associated protein, at 1,766,577 to 1,767,227 _RS27625 BPHYT_RS27635: BPHYT_RS27635 - alcohol dehydrogenase, at 1,767,832 to 1,768,872 _RS27635 BPHYT_RS27640: BPHYT_RS27640 - acetoacetyl-CoA reductase, at 1,769,304 to 1,770,050 _RS27640 BPHYT_RS27645: BPHYT_RS27645 - universal stress protein UspA, at 1,770,518 to 1,770,976 _RS27645 BPHYT_RS27650: BPHYT_RS27650 - hypothetical protein, at 1,771,035 to 1,771,382 _RS27650
Group Condition BPHYT_RS27625 BPHYT_RS27635 BPHYT_RS27640 BPHYT_RS27645 BPHYT_RS27650
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.8 -0.2 -1.0 N.D. N.D.
phage 24hr PHYTO3 MOI 1 -0.8 -0.2 -1.1 N.D. N.D.
carbon source Suberic (C) -0.3 -0.8 -0.6 N.D. N.D.
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days -0.5 -1.2 -0.0 N.D. N.D.
in planta Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 -0.5 -1.5 +0.4 N.D. N.D.
phage 24hr PHYTO3 MOI 10 -0.8 -0.3 -0.5 N.D. N.D.
phage 36hr PHYTO6 MOI 10 +0.3 -1.4 -0.3 N.D. N.D.
phage 36hr PHYTO9 MOI 1 -0.7 -0.8 +0.1 N.D. N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.2 -0.7 -1.0 N.D. N.D.
phage 36hr PHYTO2 MOI 1 +0.3 -0.8 -0.5 N.D. N.D.
phage 36hr PHYTO3 MOI 0.1 +0.4 -0.8 -0.4 N.D. N.D.
in planta Rhizosphere from sorghum RTx_430 plants, grown 2 months, low phosphate; Rep 1 +0.1 -1.8 +1.1 N.D. N.D.
phage 36hr PHYTO8 MOI 10 +0.6 -1.1 -0.1 N.D. N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.6 -0.6 -0.6 N.D. N.D.
phage 36hr PHYTO11 MOI 1 +0.5 -0.4 -0.7 N.D. N.D.
phage 36hr PHYTO7 MOI 1 -0.2 -0.8 +0.6 N.D. N.D.
replicable vector efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 -1.0 +0.1 +0.5 N.D. N.D.
phage P. phyto corn MOI 1 +0.9 -0.3 -1.0 N.D. N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.5X; growth supplemented with 0.4X LB +0.9 -0.2 -0.6 N.D. N.D.
in planta Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days +0.4 -0.8 +0.5 N.D. N.D.
nitrogen source NAG (N) +1.0 +0.1 -0.9 N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +1.4 -0.7 -0.3 N.D. N.D.
phage 36hr PHYTO9 MOI 0.1 +1.0 -0.6 -0.0 N.D. N.D.
nitrogen source Ammonium chloride (N) +0.5 +0.5 -0.5 N.D. N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 1X +0.9 +0.1 -0.5 N.D. N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.2 -0.3 +1.3 N.D. N.D.
vitamin With vitamins t2 +0.7 -0.4 +0.5 N.D. N.D.
carbon source D-Glucose 20 mM (C) +1.0 -0.4 +0.4 N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +1.3 -0.2 -0.1 N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +2.8 -0.1 -0.2 N.D. N.D.
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