Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS27445 and BPHYT_RS27450 are separated by 120 nucleotidesBPHYT_RS27450 and BPHYT_RS27455 are separated by 292 nucleotidesBPHYT_RS27455 and BPHYT_RS27460 are separated by 200 nucleotidesBPHYT_RS27460 and BPHYT_RS27465 are separated by 278 nucleotides BPHYT_RS27445: BPHYT_RS27445 - hypothetical protein, at 1,733,871 to 1,734,272 _RS27445 BPHYT_RS27450: BPHYT_RS27450 - alpha/beta hydrolase, at 1,734,393 to 1,735,196 _RS27450 BPHYT_RS27455: BPHYT_RS27455 - rod shape-determining protein MreB, at 1,735,489 to 1,736,541 _RS27455 BPHYT_RS27460: BPHYT_RS27460 - hydrolase, at 1,736,742 to 1,737,401 _RS27460 BPHYT_RS27465: BPHYT_RS27465 - DSBA oxidoreductase, at 1,737,680 to 1,739,257 _RS27465
Group Condition BPHYT_RS27445 BPHYT_RS27450 BPHYT_RS27455 BPHYT_RS27460 BPHYT_RS27465
phage 24hr PHYTO3 MOI 10 -1.3 -0.8 -2.1 N.D. +0.1
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.7 -0.7 -2.2 N.D. -0.3
phage 24hr PHYTO1 MOI 10 -0.6 -0.8 -1.8 N.D. -0.3
phage P. phyto corn MOI 0.1 -0.0 -0.1 -2.9 N.D. -0.5
tn7 efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pRH19; outgrowth in LB_gent50 -0.9 +0.1 -2.3 N.D. -0.3
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.5X; growth supplemented with 0.4X LB -1.3 -1.0 -0.5 N.D. -0.5
phage 24hr PHYTO4 MOI 0.1 -0.3 -0.5 -1.8 N.D. -0.4
phage 24hr PHYTO MOI 0.1 +0.3 -0.7 -1.9 N.D. -0.7
phage 24hr PHYTO3 MOI 10 -1.1 +0.1 -1.6 N.D. -0.3
phage 24hr PHYTO4 MOI 10 +0.4 +0.2 -1.1 N.D. -2.2
phage 36hr PHYTO8 MOI 10 -0.9 -0.5 -1.1 N.D. -0.2
phage 24hr PHYTO3 MOI 0.1 +0.3 -1.1 -1.8 N.D. -0.0
nophagecontrol 24hr Phyto Lib Only -1.3 +0.4 -1.3 N.D. -0.2
phage 36hr PHYTO3 MOI 1 -0.3 -1.1 -1.3 N.D. +0.3
phage 36hr PHYTO7 MOI 1 +0.3 -0.5 -1.7 N.D. -0.2
phage 24hr PHYTO1 MOI 10 +0.2 -0.3 -2.0 N.D. +0.3
phage 36hr PHYTO10 MOI 10 -0.1 +0.6 -1.6 N.D. -0.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant +0.6 -0.9 -1.2 N.D. +0.0
phage 24hr PHYTO2 MOI 1 +0.7 +0.4 -1.9 N.D. -0.1
phage 36hr PHYTO11 MOI 1 +0.5 -0.5 -1.2 N.D. +0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant +0.5 +0.6 -2.1 N.D. +0.2
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.5X; growth supplemented with 0.4X LB +1.0 -0.0 -1.2 N.D. -0.4
carbon source 2-Deoxy-D-Ribose (C) +0.4 +0.6 -1.4 N.D. +0.4
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB +0.7 +0.7 -1.2 N.D. +0.1
carbon source D-Glucose (C) +0.5 +0.7 -1.1 N.D. +0.4
temperature LB, 23C +0.3 -0.8 +1.2 N.D. +0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.4 -0.7 +1.0 N.D. +0.5
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +2.4 -0.7 +0.3 N.D. -0.8
in planta Plant=Arabidopsis_thaliana_FLS2; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_0.8%agar; Collection=outgrowth_LB; Time=7_days +0.9 +1.1 -0.4 N.D. +0.3
in planta Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 +0.6 -0.0 +1.2 N.D. +0.8
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