Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS27380 and BPHYT_RS27385 are separated by 200 nucleotidesBPHYT_RS27385 and BPHYT_RS27390 are separated by 281 nucleotidesBPHYT_RS27390 and BPHYT_RS27395 are separated by 25 nucleotidesBPHYT_RS27395 and BPHYT_RS27400 are separated by 68 nucleotides BPHYT_RS27380: BPHYT_RS27380 - 3-dehydroquinate dehydratase, at 1,716,576 to 1,716,683 _RS27380 BPHYT_RS27385: BPHYT_RS27385 - DeoR family transcriptional regulator, at 1,716,884 to 1,718,365 _RS27385 BPHYT_RS27390: BPHYT_RS27390 - hydroxyacid dehydrogenase, at 1,718,647 to 1,719,708 _RS27390 BPHYT_RS27395: BPHYT_RS27395 - N-acetyltransferase GCN5, at 1,719,734 to 1,720,636 _RS27395 BPHYT_RS27400: BPHYT_RS27400 - D-amino acid oxidase, at 1,720,705 to 1,722,030 _RS27400
Group Condition BPHYT_RS27380 BPHYT_RS27385 BPHYT_RS27390 BPHYT_RS27395 BPHYT_RS27400
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 1X N.D. -0.2 -2.4 -1.2 N.D.
carbon source L-Fucose (C) N.D. -0.4 -0.8 -0.8 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant N.D. -0.2 -0.7 -1.1 N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. -0.5 -1.2 -0.3 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant N.D. -0.5 +0.3 -1.7 N.D.
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. +0.3 -1.1 -0.7 N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. -0.1 +0.4 -1.5 N.D.
carbon source Malonic (C) N.D. +0.6 -0.8 -1.0 N.D.
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB N.D. +0.8 -1.2 -0.5 N.D.
nitrogen source L-Lysine (N) N.D. +0.5 -0.8 -0.6 N.D.
phage 36hr PHYTO6 MOI 10 N.D. -0.2 +0.8 -0.9 N.D.
phage 24hr PHYTO9 MOI 0.1 N.D. +0.7 +0.2 -1.0 N.D.
phage 36hr PHYTO3 MOI 0.1 N.D. +0.7 -1.0 +0.3 N.D.
supernatant control Vogels_fungal_media; pH 5.5 N.D. +0.4 -1.1 +0.8 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. -0.3 -0.7 +1.1 N.D.
nutrient casaminos N.D. +0.3 -1.1 +1.2 N.D.
nutrient L-Serine N.D. -0.7 +0.3 +0.9 N.D.
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. -0.3 -0.4 +1.2 N.D.
phage PHYTO3 MOI 0.1 N.D. -0.6 +0.6 +0.9 N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. -0.5 +1.0 +0.4 N.D.
phage 24hr PHYTO3 MOI 10 N.D. -0.2 +0.5 +1.2 N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=20h_outgrowth_LB; Time=7_days N.D. +0.2 -0.2 +1.6 N.D.
phage 36hr PHYTO6 MOI 1 N.D. +0.5 +0.7 +0.7 N.D.
carbon source Tween 20 (C) N.D. -0.0 +1.5 +0.5 N.D.
carbon source Suberic (C) N.D. +0.5 +0.5 +1.1 N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. +0.1 +1.5 +0.5 N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB N.D. +0.7 +0.9 +0.7 N.D.
carbon source adipate (C) N.D. +0.3 +0.7 +1.3 N.D.
carbon source D-Glucose (C) N.D. +0.5 +1.7 +0.4 N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth_LB; Time=7_days N.D. +0.5 +0.3 +1.7 N.D.
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