Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS27255 and BPHYT_RS27260 are separated by 274 nucleotidesBPHYT_RS27260 and BPHYT_RS27265 are separated by 295 nucleotidesBPHYT_RS27265 and BPHYT_RS27270 are separated by 689 nucleotidesBPHYT_RS27270 and BPHYT_RS27275 are separated by 295 nucleotides BPHYT_RS27255: BPHYT_RS27255 - hypothetical protein, at 1,689,621 to 1,689,800 _RS27255 BPHYT_RS27260: BPHYT_RS27260 - membrane protein, at 1,690,075 to 1,690,605 _RS27260 BPHYT_RS27265: BPHYT_RS27265 - AraC family transcriptional regulator, at 1,690,901 to 1,691,728 _RS27265 BPHYT_RS27270: BPHYT_RS27270 - photosystem reaction center subunit H, at 1,692,418 to 1,692,852 _RS27270 BPHYT_RS27275: BPHYT_RS27275 - membrane protein, at 1,693,148 to 1,693,723 _RS27275
Group Condition BPHYT_RS27255 BPHYT_RS27260 BPHYT_RS27265 BPHYT_RS27270 BPHYT_RS27275
phage 24hr PHYTO11 MOI 0.1 N.D. N.D. -0.5 -0.9 -0.4
in planta Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days N.D. N.D. -0.2 -1.4 -0.0
phage 24hr PHYTO11 MOI 1 N.D. N.D. +0.0 -1.2 -0.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant N.D. N.D. -0.3 -1.1 -0.0
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. N.D. +0.1 -0.8 -0.7
lb_dap plate control LB_DAP agar plate, outgrowth in LB N.D. N.D. -0.4 -0.9 +0.2
nitrogen source L-Serine (N) N.D. N.D. +0.3 -1.0 -0.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. N.D. +0.4 -1.4 +0.0
phage 36hr PHYTO7 MOI 1 N.D. N.D. -0.3 +0.4 -1.0
r2a control R2A control N.D. N.D. -0.2 -0.9 +0.3
nophagecontrol 36hr Phyto Lib Only N.D. N.D. +0.3 -1.0 +0.3
in planta Plant=Zea_mays; PlantTreatment=Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days N.D. N.D. -0.0 +0.7 -0.7
carbon source succinate (C) N.D. N.D. +0.3 -0.9 +0.6
carbon source Malonic (C) N.D. N.D. +0.9 -0.5 -0.1
phage 24hr PHYTO6 MOI 10 N.D. N.D. -0.5 +0.8 +0.2
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB N.D. N.D. -0.3 +0.6 +0.6
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. N.D. +0.9 -0.3 +0.3
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth; Time=14_days N.D. N.D. -0.2 +1.2 +0.2
phage 36hr PHYTO1 MOI 10 N.D. N.D. -0.2 +0.8 +0.6
phage 24hr PHYTO3 MOI 0.1 N.D. N.D. -0.2 +1.0 +0.4
motility outer cut, LB soft agar motility assay N.D. N.D. +0.6 +0.7 -0.0
supernatant control Vogels_fungal_media 0.1X N.D. N.D. -0.1 +1.1 +0.3
phage 36hr PHYTO10 MOI 10 N.D. N.D. +0.1 +0.7 +0.6
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant N.D. N.D. +0.1 +0.9 +0.4
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. N.D. -0.2 +0.5 +1.1
replicable vector efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 N.D. N.D. -0.3 +1.6 +0.3
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=14_days N.D. N.D. +0.4 +1.1 +0.3
nophagecontrol 36hr Phyto Lib only N.D. N.D. +0.3 +1.2 +0.5
carbon source 2-Deoxy-D-ribonic lithium salt (C) N.D. N.D. -0.1 +1.6 +0.6
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant N.D. N.D. -0.3 +4.5 -0.1
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