Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS27190 and BPHYT_RS27195 are separated by 39 nucleotidesBPHYT_RS27195 and BPHYT_RS27200 are separated by 68 nucleotidesBPHYT_RS27200 and BPHYT_RS27205 overlap by 4 nucleotidesBPHYT_RS27205 and BPHYT_RS27210 are separated by 13 nucleotides BPHYT_RS27190: BPHYT_RS27190 - sugar ABC transporter permease, at 1,677,881 to 1,678,912 _RS27190 BPHYT_RS27195: BPHYT_RS27195 - LacI family transcriptional regulator, at 1,678,952 to 1,679,899 _RS27195 BPHYT_RS27200: BPHYT_RS27200 - pyridoxal 4-dehydrogenase, at 1,679,968 to 1,680,984 _RS27200 BPHYT_RS27205: BPHYT_RS27205 - amidohydrolase, at 1,680,981 to 1,681,853 _RS27205 BPHYT_RS27210: BPHYT_RS27210 - alcohol dehydrogenase, at 1,681,867 to 1,682,874 _RS27210
Group Condition BPHYT_RS27190 BPHYT_RS27195 BPHYT_RS27200 BPHYT_RS27205 BPHYT_RS27210
phage 36hr PHYTO2 MOI 1 -0.8 +0.8 -0.6 N.D. -1.0
phage 24hr PHYTO3 MOI 10 +0.1 +0.6 -1.0 N.D. -1.3
phage P. phyto PHYTO2 MOI 1 -0.8 +0.5 -0.2 N.D. -0.7
phage 36hr PHYTO1 MOI 10 -0.7 +0.5 -0.7 N.D. -0.3
phage 24hr PHYTO3 MOI 0.1 -0.3 +0.7 -0.2 N.D. -0.9
phage 36hr PHYTO6 MOI 0.1 -1.5 +0.5 +0.0 N.D. +0.4
replicable vector efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 -0.4 +0.3 +0.8 N.D. -1.2
motility inner cut, LB soft agar motility assay -0.7 +0.9 -0.4 N.D. -0.1
phage 24hr PHYTO6 MOI 10 -1.0 +1.0 -0.2 N.D. -0.0
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.5X; growth supplemented with 0.4X LB +0.3 +0.7 -0.6 N.D. -0.5
phage 36hr PHYTO1 MOI 1 -0.0 +1.0 +0.2 N.D. -1.1
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth_LB; Time=7_days -0.2 +1.1 -0.6 N.D. -0.2
in planta Rhizosphere from sorghum RTx_430 plants, grown 2 months, low phosphate; Rep 1 -0.8 +0.6 +0.5 N.D. -0.2
in planta Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -1.0 +0.4 +0.6 N.D. +0.3
phage 36hr PHYTO3 MOI 1 -0.5 +1.1 +0.2 N.D. -0.6
in planta Plant=Zea_mays; PlantTreatment=None; Sample=soil; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.8 +0.5 +0.6 N.D. +0.2
phage 36hr PHYTO1 MOI 1 +0.1 +1.2 -0.3 N.D. -0.3
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.7 +0.7 -0.2 N.D. +0.9
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=20h_outgrowth_LB; Time=7_days -0.3 +1.3 +0.1 N.D. -0.4
phage 24hr PHYTO10 MOI 1 +0.4 +0.8 +0.1 N.D. -0.5
in planta Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.5 +0.6 +1.0 N.D. -0.2
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.6 +0.6 +0.3 N.D. -0.6
phage 24hr PHYTO3 MOI 10 -0.0 +0.7 -0.4 N.D. +0.8
carbon source Malonic (C) -0.6 +2.1 +0.4 N.D. -0.7
phage 24hr PHYTO4 MOI 10 +0.2 +0.9 -0.5 N.D. +0.8
in planta Plant=Zea_mays; PlantTreatment=None; Sample=soil; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.2 +0.4 +1.1 N.D. +0.3
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.3 +0.8 +1.0 N.D. -0.1
carbon source 2-Deoxy-D-ribonic lithium salt (C) +0.5 +1.0 -0.0 N.D. +0.4
lb_dap plate control LB_DAP agar plate, outgrowth in LB +0.3 +0.9 +0.3 N.D. +0.6
lb_dap plate control and outgrow on gent LB_DAP agar plate, outgrowth in LB_gent +0.7 +0.9 +0.8 N.D. +0.1
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