Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS27090 and BPHYT_RS27095 are separated by 204 nucleotidesBPHYT_RS27095 and BPHYT_RS27100 are separated by 227 nucleotidesBPHYT_RS27100 and BPHYT_RS27105 are separated by 87 nucleotidesBPHYT_RS27105 and BPHYT_RS27110 are separated by 435 nucleotides BPHYT_RS27090: BPHYT_RS27090 - aldo/keto reductase, at 1,650,028 to 1,651,080 _RS27090 BPHYT_RS27095: BPHYT_RS27095 - ATP-dependent DNA helicase, at 1,651,285 to 1,655,799 _RS27095 BPHYT_RS27100: BPHYT_RS27100 - RNase II stability modulator, at 1,656,027 to 1,658,030 _RS27100 BPHYT_RS27105: BPHYT_RS27105 - enoyl-CoA hydratase, at 1,658,118 to 1,659,002 _RS27105 BPHYT_RS27110: BPHYT_RS27110 - dehydrogenase, at 1,659,438 to 1,660,541 _RS27110
Group Condition BPHYT_RS27090 BPHYT_RS27095 BPHYT_RS27100 BPHYT_RS27105 BPHYT_RS27110
carbon source Xylitol (C) -0.7 +0.2 -2.5 +0.5 +0.1
carbon source L-Fucose (C) -1.0 +0.3 -1.5 +0.2 +0.2
carbon source D-Xylose (C) -0.2 +0.2 -2.5 +0.7 +0.1
phage 24hr PHYTO10 MOI 1 -1.2 -0.4 +0.3 -0.0 -0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant -0.6 -0.4 +0.4 -0.1 -0.7
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.5X; growth supplemented with 0.4X LB +0.4 -0.2 -0.3 -0.6 -0.6
phage 36hr PHYTO1 MOI 1 -1.1 -0.3 +0.2 +0.3 -0.3
phage 36hr PHYTO11 MOI 1 -0.9 -0.0 +0.6 -0.1 -0.4
agar plate interaction control Parafilmed volatile agar plate with no fungus -1.0 -0.5 +0.3 +0.4 +0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant -0.9 +0.3 +0.0 -0.6 +0.5
phage 24hr PHYTO9 MOI 10 -0.7 -0.7 -0.1 +0.1 +0.7
phage 36hr PHYTO4 MOI 0.1 -1.4 +0.1 +0.5 +0.1 +0.1
motility inner cut, LB soft agar motility assay -0.9 -0.3 -0.1 +0.3 +0.5
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.9 -0.0 +0.8 +0.2 -0.4
in planta Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days +0.7 -0.1 -0.9 +0.7 -0.6
carbon source 2-Deoxy-D-ribonic lithium salt (C) +0.5 +0.3 -0.4 +0.3 -0.7
phage 24hr PHYTO3 MOI 10 -1.0 +0.4 +0.4 +0.2 +0.1
in planta Rhizosphere from sorghum RTx_430 plants, grown 2 months, low phosphate; Rep 1 +0.2 -0.6 +0.8 -0.3 +0.1
phage 24hr PHYTO6 MOI 10 -1.1 +0.3 +0.5 +0.3 +0.3
phage P. phyto PHYTO2 MOI 0.1 -1.2 +0.1 +0.6 +0.5 +0.3
phage 36hr PHYTO MOI 0.1 -1.0 +0.3 +0.4 +0.3 +0.2
phage 36hr PHYTO8 MOI 10 -0.8 +0.2 +0.4 +0.6 +0.4
carbon source Malonic (C) +0.2 +0.4 -0.6 -0.0 +1.1
in planta Plant=Zea_mays; PlantTreatment=None; sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_R2A_with_Tween_and_Cycloheximide; Time=15_days -0.5 -0.1 +1.8 -0.3 +0.2
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.1 +1.2 -0.6 -0.2 +0.9
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.4 +0.5 +0.6 +0.0 +0.6
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.5 -0.3 +1.8 -0.2 +0.6
phage 36hr PHYTO10 MOI 10 -0.3 +0.2 +0.6 +0.8 +0.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.8 -0.4 +1.0 +0.1 +0.4
lb_dap plate control and outgrow on gent LB_DAP agar plate, outgrowth in LB_gent +0.3 +0.3 +0.7 +0.5 +0.3
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