Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS27050 and BPHYT_RS27055 are separated by 90 nucleotidesBPHYT_RS27055 and BPHYT_RS27060 are separated by 347 nucleotidesBPHYT_RS27060 and BPHYT_RS27065 are separated by 162 nucleotidesBPHYT_RS27065 and BPHYT_RS27070 are separated by 91 nucleotides BPHYT_RS27050: BPHYT_RS27050 - DNA topoisomerase, at 1,643,363 to 1,644,682 _RS27050 BPHYT_RS27055: BPHYT_RS27055 - hypothetical protein, at 1,644,773 to 1,645,066 _RS27055 BPHYT_RS27060: BPHYT_RS27060 - photosystem reaction center subunit H, at 1,645,414 to 1,645,911 _RS27060 BPHYT_RS27065: BPHYT_RS27065 - hypothetical protein, at 1,646,074 to 1,646,463 _RS27065 BPHYT_RS27070: BPHYT_RS27070 - cation transporter, at 1,646,555 to 1,647,874 _RS27070
Group Condition BPHYT_RS27050 BPHYT_RS27055 BPHYT_RS27060 BPHYT_RS27065 BPHYT_RS27070
phage 24hr PHYTO9 MOI 0.1 -0.5 N.D. -3.8 +0.3 -0.2
phage 24hr PHYTO7 MOI 1 +0.2 N.D. -3.7 -0.5 +0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -0.0 N.D. -2.2 -1.4 -0.2
phage 24hr PHYTO7 MOI 10 -0.2 N.D. -1.2 -2.0 +0.1
carbon source Protocatechuic Acid (C) -0.7 N.D. -1.9 -0.4 -0.2
vitamin No vitamins t2 +0.2 N.D. -0.2 -2.8 +0.2
in planta Plant=Zea_mays; PlantTreatment=Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days -0.3 N.D. -1.1 -1.3 +0.1
nitrogen source Uridine (N) -0.2 N.D. -1.1 -1.1 -0.1
phage 36hr PHYTO9 MOI 0.1 +0.5 N.D. -2.0 -1.3 +0.3
carbon source adipate (C) -0.6 N.D. -2.3 +0.4 +0.1
phage 36hr PHYTO11 MOI 1 +0.1 N.D. -2.0 -0.3 -0.2
vitamin No vitamins t1 +0.1 N.D. -0.8 -1.8 +0.1
phage 24hr PHYTO1 MOI 10 +0.1 N.D. -1.0 -1.2 -0.2
phage P. phyto corn MOI 0.1 -0.4 N.D. -2.0 -0.1 +0.2
phage 24hr PHYTO3 MOI 1 -0.4 N.D. -1.9 +0.1 +0.1
phage P. phyto PHYTO2 MOI 1 +0.1 N.D. -2.5 +0.4 -0.1
phage 36hr PHYTO1 MOI 10 +0.2 N.D. -2.1 -0.2 +0.2
carbon source Deoxyribonucleic from herring sperm 10 mg/mL (C) +0.6 N.D. -2.1 -0.9 +0.4
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.1 N.D. -2.3 +0.7 -0.1
nophagecontrol 36hr Phyto Lib only -0.4 N.D. -1.8 +0.4 +0.0
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.3 N.D. -1.4 -0.9 +0.3
nitrogen source NAG (N) -0.4 N.D. -1.7 +0.3 +0.1
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.0 N.D. -1.1 -0.9 +0.4
vitamin With vitamins t1 +0.5 N.D. -0.9 -1.2 +0.1
vitamin With vitamins t2 +0.5 N.D. -0.7 -1.0 +0.2
nophagecontrol 24hr Phyto Lib Only +0.6 N.D. -1.5 -0.5 +0.4
tn7 efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pRH19; outgrowth in LB_gent50 +0.3 N.D. +1.4 -1.5 +0.3
phage 24hr PHYTO6 MOI 10 +0.3 N.D. +1.7 -0.6 +0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant +0.2 N.D. +1.3 +1.1 -0.4
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.0 N.D. +0.7 +1.2 +0.6
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