Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS26990 and BPHYT_RS26995 are separated by 183 nucleotidesBPHYT_RS26995 and BPHYT_RS27000 are separated by 187 nucleotidesBPHYT_RS27000 and BPHYT_RS27005 are separated by 137 nucleotidesBPHYT_RS27005 and BPHYT_RS27010 are separated by 512 nucleotides BPHYT_RS26990: BPHYT_RS26990 - GntR family transcriptional regulator, at 1,632,521 to 1,633,267 _RS26990 BPHYT_RS26995: BPHYT_RS26995 - peptidase S10, at 1,633,451 to 1,635,223 _RS26995 BPHYT_RS27000: BPHYT_RS27000 - aspartate aminotransferase, at 1,635,411 to 1,636,589 _RS27000 BPHYT_RS27005: BPHYT_RS27005 - RNA polymerase sigma54 factor, at 1,636,727 to 1,638,208 _RS27005 BPHYT_RS27010: BPHYT_RS27010 - transporter, at 1,638,721 to 1,639,062 _RS27010
Group Condition BPHYT_RS26990 BPHYT_RS26995 BPHYT_RS27000 BPHYT_RS27005 BPHYT_RS27010
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.7 -0.7 -0.2 -0.9 N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.8 -0.6 -0.4 -0.4 N.D.
carbon source Phenylacetic 5 mM (C) -0.7 -0.5 -0.1 -0.8 N.D.
supernatant control Vogels_fungal_media 0.1X -1.1 -0.1 -0.3 -0.4 N.D.
phage 36hr PHYTO11 MOI 0.1 -0.0 -0.8 -0.7 -0.4 N.D.
phage 36hr PHYTO1 MOI 10 -1.4 +0.1 -0.8 +0.2 N.D.
phage 36hr PHYTO10 MOI 10 -1.5 +0.3 -0.1 -0.3 N.D.
phage 36hr PHYTO1 MOI 1 -1.3 +0.1 +0.2 -0.6 N.D.
carbon source Sebacic (C) -0.4 -0.3 +0.0 -0.8 N.D.
phage 24hr PHYTO4 MOI 10 -1.1 +0.1 -0.1 -0.2 N.D.
phage 24hr PHYTO1 MOI 10 -1.2 +0.3 +0.1 -0.4 N.D.
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.5 -0.2 +0.3 -0.7 N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.8 +0.5 +0.1 -0.9 N.D.
lb_dap plate control and outgrow on gent LB_DAP agar plate, outgrowth in LB_gent -0.8 +0.4 -0.2 -0.5 N.D.
motility outer cut, LB soft agar motility assay -0.6 +0.5 -0.1 -0.7 N.D.
in planta Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 +0.5 -0.1 -0.2 -1.0 N.D.
phage 24hr PHYTO3 MOI 1 -1.0 +0.6 -0.3 -0.0 N.D.
phage P. phyto PHYTO2 MOI 0.1 -0.8 +0.3 -0.4 +0.2 N.D.
phage 36hr PHYTO4 MOI 1 -0.4 +0.6 -0.5 -0.4 N.D.
nophagecontrol 36hr Phyto Lib only -1.2 +0.6 +0.1 +0.1 N.D.
phage 36hr PHYTO9 MOI 0.1 -0.8 +0.3 +0.4 -0.4 N.D.
phage 36hr PHYTO3 MOI 1 -0.7 +0.8 -0.3 -0.0 N.D.
phage 36hr PHYTO2 MOI 1 -0.6 +0.6 -0.3 +0.2 N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB +0.0 +1.1 -0.5 -0.4 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant +0.3 +0.8 +0.2 -1.0 N.D.
carbon source L-Lactate (C) +1.3 -0.0 +0.0 -0.7 N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.6 +0.7 +0.4 +0.3 N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.5 +0.7 +0.5 -0.0 N.D.
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +1.2 +0.2 +0.2 -0.5 N.D.
vitamin With vitamins t2 -0.3 +1.1 +0.1 +0.2 N.D.
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