Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS26950 and BPHYT_RS26955 are separated by 37 nucleotidesBPHYT_RS26955 and BPHYT_RS26960 are separated by 155 nucleotidesBPHYT_RS26960 and BPHYT_RS26965 are separated by 73 nucleotidesBPHYT_RS26965 and BPHYT_RS26970 overlap by 14 nucleotides BPHYT_RS26950: BPHYT_RS26950 - MFS transporter, at 1,623,963 to 1,625,378 _RS26950 BPHYT_RS26955: BPHYT_RS26955 - succinylglutamate desuccinylase, at 1,625,416 to 1,626,429 _RS26955 BPHYT_RS26960: BPHYT_RS26960 - LysR family transcriptional regulator, at 1,626,585 to 1,627,469 _RS26960 BPHYT_RS26965: BPHYT_RS26965 - L-lactate dehydrogenase, LldG subunit (from data), at 1,627,543 to 1,628,130 _RS26965 BPHYT_RS26970: BPHYT_RS26970 - L-lactate dehydrogenase, LldF subunit (from data), at 1,628,117 to 1,629,511 _RS26970
Group Condition BPHYT_RS26950 BPHYT_RS26955 BPHYT_RS26960 BPHYT_RS26965 BPHYT_RS26970
carbon source L-Lactate (C) +0.3 -0.6 +0.0 -4.2 -5.3
carbon source D,L-Lactate (C) -0.1 -1.2 -0.0 -4.5 -1.8
carbon source L-Fucose (C) +0.4 +1.0 +0.3 -5.3 -2.6
nitrogen source L-Alanine (N) -0.0 -4.7 -0.4 +0.1 -0.4
carbon source L-Rhamnose (C) -0.2 -0.2 -0.5 -2.7 -1.7
carbon source D,L-Lactate (C) -0.2 -1.5 +0.7 -1.6 -1.6
carbon source D,L-Lactate (C) +0.1 -0.5 -0.4 -1.7 -1.6
carbon source D-Glucose (C) -0.3 -1.8 +0.1 -2.0 -0.0
carbon source 2-Deoxy-D-ribonic lithium salt (C) +0.0 -4.1 +0.4 -0.2 -0.0
in planta Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days -0.3 -1.8 +0.5 -2.1 -0.2
in planta Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 -0.7 -1.6 -0.7 -0.5 -0.1
carbon source D,L-Lactate (C) +0.3 +0.4 -0.2 -1.9 -1.6
supernatant control Vogels_fungal_media; pH 5.5 -0.5 -1.4 +0.4 -0.3 -0.8
carbon source D,L-Lactate (C) +0.0 +0.8 -0.5 -1.4 -1.2
nitrogen source NAG (N) +0.3 -2.9 +0.3 +0.4 -0.2
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB -0.7 +1.2 +0.7 -2.3 -0.3
in planta Rhizosphere from sorghum RTx_430 plants, grown 2 months, low phosphate; Rep 1 -0.1 -2.4 +0.3 +0.4 +0.4
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.7 -0.3 +1.1 -3.0 +0.2
nitrogen source L-Proline (N) +0.6 -0.1 +0.4 -2.2 -0.1
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth; Time=14_days -0.2 +1.5 -0.4 -2.5 +0.2
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days -0.6 +0.8 +0.6 -1.0 -0.5
carbon source Phenylacetic 5 mM (C) -0.3 -2.0 -0.0 +1.5 +0.1
nitrogen source L-Lysine (N) +0.5 +0.9 -0.4 -1.4 -0.3
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.6 -1.8 +0.9 +0.7 +0.4
phage 36hr PHYTO MOI 0.1 +0.2 +1.1 +0.0 -1.6 +0.5
nitrogen source Putrescine (N) +0.3 -1.3 +0.5 +1.2 -0.2
carbon source Deoxyribonucleic from herring sperm 10 mg/mL (C) -0.3 -1.0 -0.2 +2.7 -0.3
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.5X; growth supplemented with 0.4X LB -0.7 +2.5 +0.7 +0.1 -0.5
carbon source D-Glucose (C) +0.6 +1.8 +0.3 +0.9 +0.1
motility inner cut, LB soft agar motility assay +0.9 +1.4 +1.1 +0.7 +0.7
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