Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS26890 and BPHYT_RS26895 are separated by 151 nucleotidesBPHYT_RS26895 and BPHYT_RS26900 are separated by 82 nucleotidesBPHYT_RS26900 and BPHYT_RS26905 are separated by 214 nucleotidesBPHYT_RS26905 and BPHYT_RS26910 are separated by 67 nucleotides BPHYT_RS26890: BPHYT_RS26890 - LysR family transcriptional regulator, at 1,605,269 to 1,606,144 _RS26890 BPHYT_RS26895: BPHYT_RS26895 - nicotinate phosphoribosyltransferase, at 1,606,296 to 1,607,723 _RS26895 BPHYT_RS26900: BPHYT_RS26900 - isoprenylcysteine carboxyl methyltransferase, at 1,607,806 to 1,608,492 _RS26900 BPHYT_RS26905: BPHYT_RS26905 - pilus assembly protein CpaE, at 1,608,707 to 1,609,909 _RS26905 BPHYT_RS26910: BPHYT_RS26910 - hypothetical protein, at 1,609,977 to 1,610,264 _RS26910
Group Condition BPHYT_RS26890 BPHYT_RS26895 BPHYT_RS26900 BPHYT_RS26905 BPHYT_RS26910
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB -0.2 -1.0 -1.1 N.D. -1.5
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 1X -1.1 -0.8 -0.0 N.D. -0.8
phage 24hr PHYTO7 MOI 1 -0.0 +0.5 -1.4 N.D. -0.7
phage P. phyto PHYTO2 MOI 1 +0.1 +0.5 -0.9 N.D. -0.8
nophagecontrol 36hr Phyto Lib only -0.1 -0.2 -1.3 N.D. +0.8
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB +0.2 -1.1 +0.6 N.D. +0.3
nophagecontrol 24hr Phyto Lib only -0.3 +0.4 -0.8 N.D. +0.9
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant +0.4 -1.2 +0.2 N.D. +0.8
phage 24hr PHYTO4 MOI 10 +0.6 -0.2 -0.9 N.D. +0.8
phage 36hr PHYTO MOI 0.1 +0.5 +0.5 -1.0 N.D. +0.3
phage P. phyto corn MOI 1 +0.8 -0.3 -0.7 N.D. +0.4
phage 36hr PHYTO4 MOI 0.1 +0.6 +0.4 -1.0 N.D. +0.6
phage 36hr PHYTO6 MOI 1 +0.1 +0.9 -0.9 N.D. +0.8
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant +0.5 -0.6 +0.2 N.D. +0.9
in planta Plant=Arabidopsis_thaliana_FLS2; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_0.8%agar; Collection=outgrowth_LB; Time=7_days +0.1 +0.6 +0.9 N.D. -0.5
nophagecontrol 24hr Phyto Lib Only +0.7 +0.7 -0.6 N.D. +0.3
phage 24hr PHYTO MOI 0.1 +0.4 +0.4 -0.4 N.D. +0.9
phage 24hr PHYTO4 MOI 0.1 +0.3 +0.7 -0.8 N.D. +1.4
nophagecontrol 24hr Phyto Lib Only +0.4 +0.8 -0.4 N.D. +0.9
phage 24hr PHYTO8 MOI 1 +0.6 +0.8 -0.3 N.D. +0.6
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.2 -0.7 +0.0 N.D. +2.2
phage 36hr PHYTO10 MOI 1 +0.5 +0.7 -0.6 N.D. +1.2
carbon source adipate (C) +0.8 +0.6 -0.3 N.D. +0.9
carbon source Protocatechuic Acid (C) +1.0 +0.7 -0.2 N.D. +0.7
carbon source Protocatechuic Acid (C) +0.7 +1.0 -0.4 N.D. +0.9
carbon source D-Glucose (C) +0.3 +0.5 +0.6 N.D. +0.8
carbon source Suberic (C) +0.6 +1.2 -0.2 N.D. +0.8
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant +0.9 +0.4 +0.2 N.D. +0.9
phage 36hr PHYTO8 MOI 10 +0.6 +1.1 -0.3 N.D. +1.0
motility inner cut, LB soft agar motility assay +1.0 +0.5 +0.6 N.D. +0.3
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