Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS26805 and BPHYT_RS26810 are separated by 148 nucleotidesBPHYT_RS26810 and BPHYT_RS26815 are separated by 164 nucleotidesBPHYT_RS26815 and BPHYT_RS26820 overlap by 4 nucleotidesBPHYT_RS26820 and BPHYT_RS26825 are separated by 131 nucleotides BPHYT_RS26805: BPHYT_RS26805 - methionine sulfoxide reductase B, at 1,586,922 to 1,587,446 _RS26805 BPHYT_RS26810: BPHYT_RS26810 - AsnC family transcriptional regulator, at 1,587,595 to 1,588,122 _RS26810 BPHYT_RS26815: BPHYT_RS26815 - 1-aminocyclopropane-1-carboxylate deaminase, at 1,588,287 to 1,589,303 _RS26815 BPHYT_RS26820: BPHYT_RS26820 - LysR family transcriptional regulator, at 1,589,300 to 1,590,187 _RS26820 BPHYT_RS26825: BPHYT_RS26825 - quercetin 2,3-dioxygenase, at 1,590,319 to 1,591,044 _RS26825
Group Condition BPHYT_RS26805 BPHYT_RS26810 BPHYT_RS26815 BPHYT_RS26820 BPHYT_RS26825
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. N.D. -4.3 +0.3 -1.1
phage P. phyto PHYTO2 MOI 0.1 N.D. N.D. -1.4 -2.8 -0.1
phage P. phyto PHYTO2 MOI 1 N.D. N.D. -2.2 -1.1 -0.9
phage 24hr PHYTO MOI 0.1 N.D. N.D. -1.6 -1.4 -0.9
phage 36hr PHYTO6 MOI 0.1 N.D. N.D. -2.2 -0.8 -0.9
phage 24hr PHYTO9 MOI 0.1 N.D. N.D. +0.7 -3.0 -1.2
phage 36hr PHYTO4 MOI 1 N.D. N.D. -0.4 -2.3 -0.8
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant N.D. N.D. -0.7 -2.2 -0.4
phage 24hr PHYTO2 MOI 0.1 N.D. N.D. -0.8 -2.7 +0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant N.D. N.D. +0.2 -2.0 -1.3
phage 24hr PHYTO6 MOI 10 N.D. N.D. -0.8 -1.8 -0.4
phage 36hr PHYTO1 MOI 10 N.D. N.D. -0.7 -1.4 -0.8
phage 36hr PHYTO3 MOI 1 N.D. N.D. -0.4 -2.6 +0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant N.D. N.D. -0.5 -1.8 -0.5
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. N.D. -0.5 -1.3 -0.6
phage 36hr PHYTO9 MOI 0.1 N.D. N.D. -0.9 -0.8 -0.7
phage 24hr PHYTO11 MOI 0.1 N.D. N.D. -0.9 -0.2 -1.3
nophagecontrol 24hr Phyto Lib Only N.D. N.D. -0.4 -0.3 -1.7
phage P. phyto PHYTO2 MOI 1 N.D. N.D. -1.2 -1.4 +0.3
phage 24hr PHYTO3 MOI 10 N.D. N.D. -2.5 +0.6 -0.3
phage 24hr PHYTO8 MOI 1 N.D. N.D. +0.2 -2.1 -0.1
phage 36hr PHYTO3 MOI 1 N.D. N.D. -1.5 +0.3 -0.8
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 1X N.D. N.D. +0.6 -1.5 -0.4
supernatant control Vogels_fungal_media; pH 5.5 N.D. N.D. +0.7 -0.9 -0.9
carbon source Phenylacetic 5 mM (C) N.D. N.D. -0.9 -0.5 +1.0
nitrogen source Uridine (N) N.D. N.D. +1.0 -1.4 -0.0
in planta Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. N.D. +0.8 -1.5 +0.7
nophagecontrol 36hr Phyto Lib only N.D. N.D. -1.0 +0.9 +0.5
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. N.D. +1.2 +0.3 -0.9
lb_dap plate control and outgrow on gent LB_DAP agar plate, outgrowth in LB_gent N.D. N.D. +0.5 +1.4 +1.0
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