Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS26775 and BPHYT_RS26780 are separated by 10 nucleotidesBPHYT_RS26780 and BPHYT_RS26785 are separated by 18 nucleotidesBPHYT_RS26785 and BPHYT_RS26790 are separated by 634 nucleotidesBPHYT_RS26790 and BPHYT_RS26795 are separated by 205 nucleotides BPHYT_RS26775: BPHYT_RS26775 - chemotaxis protein CheB, at 1,576,835 to 1,577,449 _RS26775 BPHYT_RS26780: BPHYT_RS26780 - chemotaxis protein CheR, at 1,577,460 to 1,578,305 _RS26780 BPHYT_RS26785: BPHYT_RS26785 - chemotaxis protein CheY, at 1,578,324 to 1,581,911 _RS26785 BPHYT_RS26790: BPHYT_RS26790 - hydroperoxidase II, at 1,582,546 to 1,584,678 _RS26790 BPHYT_RS26795: BPHYT_RS26795 - MltA-interacting MipA family protein, at 1,584,884 to 1,585,780 _RS26795
Group Condition BPHYT_RS26775 BPHYT_RS26780 BPHYT_RS26785 BPHYT_RS26790 BPHYT_RS26795
lb_dap plate control and outgrow on gent LB_DAP agar plate, outgrowth in LB_gent -3.0 +0.4 -1.2 -0.2 -0.6
carbon source 2-Deoxy-D-ribonic lithium salt (C) -2.6 -0.0 -0.6 -0.0 +0.1
carbon source D-Glucose (C) -3.0 +0.3 -0.5 +0.0 +0.2
supernatant control Vogels_fungal_media 0.1X -2.4 +0.4 -0.4 -0.1 -0.0
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB -1.2 -0.1 -0.4 -0.3 -0.5
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days -1.6 +0.2 -0.3 -0.3 +0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -1.8 +0.4 -0.2 -0.0 +0.1
phage 36hr PHYTO7 MOI 0.1 -1.5 +0.6 -0.1 +0.0 -0.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -1.8 +0.0 +0.5 -0.1 -0.0
carbon source D-Glucose 20 mM (C) -1.4 +0.2 -0.6 +0.2 +0.2
phage 36hr PHYTO1 MOI 10 +0.1 +0.6 -1.0 -0.3 -0.6
phage 36hr PHYTO7 MOI 1 -1.7 +0.6 +0.2 -0.2 -0.0
phage 24hr PHYTO3 MOI 10 -1.2 +1.0 -0.2 -0.4 -0.4
phage 24hr PHYTO10 MOI 1 -2.0 +0.9 +0.1 -0.1 -0.0
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant -0.8 +0.5 -0.5 +0.2 -0.2
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.8X; growth supplemented with 0.4X LB -1.3 +1.0 -0.1 -0.1 +0.0
phage 24hr PHYTO6 MOI 10 -0.9 +0.8 -0.1 +0.2 -0.3
phage 36hr PHYTO11 MOI 0.1 -0.7 +1.0 -0.3 -0.1 -0.2
phage 36hr PHYTO MOI 0.1 -1.0 +0.9 +0.1 -0.3 +0.2
phage 36hr PHYTO9 MOI 1 -1.3 +1.0 -0.1 -0.1 +0.3
phage 24hr PHYTO4 MOI 10 +0.4 +1.0 -0.2 -0.3 -0.6
nitrogen source L-Asparagine (N) +1.8 -0.3 -0.2 -0.1 +0.0
phage 24hr PHYTO7 MOI 10 +1.2 +0.4 +0.2 -0.2 -0.4
phage 36hr PHYTO11 MOI 1 +1.2 +1.1 -0.1 -0.1 -0.6
phage 36hr PHYTO3 MOI 1 +0.9 +1.0 -0.2 +0.2 -0.2
phage 24hr PHYTO2 MOI 0.1 +1.0 +1.0 +0.1 +0.0 -0.3
nophagecontrol 24hr Phyto Lib only +1.1 +0.7 +0.3 -0.3 +0.1
nitrogen source L-Isoleucine (N) +2.0 +0.2 +0.0 -0.2 -0.0
phage 36hr PHYTO11 MOI 0.1 +0.7 +1.1 -0.0 +0.3 +0.3
phage 36hr PHYTO2 MOI 1 +1.6 +1.2 +0.4 -0.7 +0.3
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