Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS26590 and BPHYT_RS26595 are separated by 94 nucleotidesBPHYT_RS26595 and BPHYT_RS26600 are separated by 118 nucleotidesBPHYT_RS26600 and BPHYT_RS26605 are separated by 329 nucleotidesBPHYT_RS26605 and BPHYT_RS26610 are separated by 145 nucleotides BPHYT_RS26590: BPHYT_RS26590 - DNA-3-methyladenine glycosylase, at 1,534,759 to 1,535,379 _RS26590 BPHYT_RS26595: BPHYT_RS26595 - carbonic anhydrase, at 1,535,474 to 1,536,211 _RS26595 BPHYT_RS26600: BPHYT_RS26600 - carbonic anhydrase, at 1,536,330 to 1,536,965 _RS26600 BPHYT_RS26605: BPHYT_RS26605 - hypothetical protein, at 1,537,295 to 1,537,603 _RS26605 BPHYT_RS26610: BPHYT_RS26610 - porin, at 1,537,749 to 1,538,900 _RS26610
Group Condition BPHYT_RS26590 BPHYT_RS26595 BPHYT_RS26600 BPHYT_RS26605 BPHYT_RS26610
phage P. phyto PHYTO2 MOI 1 -0.3 -2.5 N.D. N.D. +0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant +0.6 -1.7 N.D. N.D. -0.4
phage 24hr PHYTO MOI 0.1 -0.5 -0.8 N.D. N.D. +0.0
in planta Rhizosphere from sorghum RTx_430 plants, grown 2 months, low phosphate; Rep 1 +0.4 -0.8 N.D. N.D. -0.5
carbon source Protocatechuic Acid (C) +0.5 -0.9 N.D. N.D. -0.1
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.3 -0.9 N.D. N.D. +0.2
in planta Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 +0.5 -1.2 N.D. N.D. +0.2
in planta Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.0 -1.0 N.D. N.D. +0.5
phage 36hr PHYTO2 MOI 0.1 +0.7 -0.9 N.D. N.D. -0.3
carbon source Sebacic (C) +0.4 -0.9 N.D. N.D. +0.1
phage 24hr PHYTO3 MOI 10 +0.6 -0.2 N.D. N.D. -0.7
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.2 -0.9 N.D. N.D. +0.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant +0.6 -0.8 N.D. N.D. +0.1
phage 24hr PHYTO1 MOI 10 +0.4 -0.8 N.D. N.D. +0.3
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.2 -0.8 N.D. N.D. +0.5
phage 24hr PHYTO6 MOI 0.1 +0.7 -0.6 N.D. N.D. +0.1
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_0.8%agar; Collection=outgrowth_LB; Time=7_days +0.2 +0.8 N.D. N.D. -0.6
phage 24hr PHYTO1 MOI 1 +1.0 -0.3 N.D. N.D. +0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant -0.5 +0.7 N.D. N.D. +0.7
phage 24hr PHYTO MOI 0.1 +0.7 +0.6 N.D. N.D. -0.2
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_0.8%agar; Collection=outgrowth_LB; Time=7_days +0.4 +1.1 N.D. N.D. -0.2
phage 36hr PHYTO6 MOI 0.1 -0.1 +1.1 N.D. N.D. +0.3
nophagecontrol 36hr Phyto Lib Only +0.7 +0.5 N.D. N.D. +0.3
phage 24hr PHYTO2 MOI 0.1 +0.4 +1.3 N.D. N.D. -0.1
phage 36hr PHYTO6 MOI 0.1 +0.1 +1.0 N.D. N.D. +0.4
phage 36hr PHYTO8 MOI 10 +0.4 +0.8 N.D. N.D. +0.4
phage 36hr PHYTO2 MOI 1 +0.8 +0.9 N.D. N.D. +0.0
phage 24hr PHYTO6 MOI 10 +0.7 +1.1 N.D. N.D. -0.1
phage 36hr PHYTO10 MOI 10 +0.3 +1.1 N.D. N.D. +0.6
phage 24hr PHYTO4 MOI 10 +1.0 +1.0 N.D. N.D. +0.4
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