Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS26580 and BPHYT_RS26585 are separated by 447 nucleotidesBPHYT_RS26585 and BPHYT_RS26590 are separated by 92 nucleotidesBPHYT_RS26590 and BPHYT_RS26595 are separated by 94 nucleotidesBPHYT_RS26595 and BPHYT_RS26600 are separated by 118 nucleotides BPHYT_RS26580: BPHYT_RS26580 - hypothetical protein, at 1,531,646 to 1,532,545 _RS26580 BPHYT_RS26585: BPHYT_RS26585 - trehalase, at 1,532,993 to 1,534,666 _RS26585 BPHYT_RS26590: BPHYT_RS26590 - DNA-3-methyladenine glycosylase, at 1,534,759 to 1,535,379 _RS26590 BPHYT_RS26595: BPHYT_RS26595 - carbonic anhydrase, at 1,535,474 to 1,536,211 _RS26595 BPHYT_RS26600: BPHYT_RS26600 - carbonic anhydrase, at 1,536,330 to 1,536,965 _RS26600
Group Condition BPHYT_RS26580 BPHYT_RS26585 BPHYT_RS26590 BPHYT_RS26595 BPHYT_RS26600
phage P. phyto PHYTO2 MOI 1 N.D. -0.8 -0.3 -2.5 N.D.
replicable vector efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 N.D. -0.4 -0.7 -0.3 N.D.
phage 24hr PHYTO MOI 0.1 N.D. -0.0 -0.5 -0.8 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant N.D. -0.1 +0.6 -1.7 N.D.
replicable vector efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 N.D. +0.3 -0.3 -1.0 N.D.
phage 24hr PHYTO1 MOI 10 N.D. -0.3 +0.4 -0.8 N.D.
phage 36hr PHYTO8 MOI 10 N.D. -1.2 +0.5 +0.1 N.D.
nophagecontrol 36hr Phyto Lib only N.D. -0.4 +0.6 -0.4 N.D.
carbon source Protocatechuic Acid (C) N.D. +0.2 +0.5 -0.9 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant N.D. +0.2 +0.6 -0.8 N.D.
carbon source Sebacic (C) N.D. +0.3 +0.4 -0.9 N.D.
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. +0.7 +0.3 -0.9 N.D.
in planta Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 N.D. +0.8 +0.5 -1.2 N.D.
phage 36hr PHYTO2 MOI 0.1 N.D. +0.3 +0.7 -0.9 N.D.
phage 36hr PHYTO11 MOI 0.1 N.D. -1.1 +0.1 +1.2 N.D.
phage 24hr PHYTO6 MOI 0.1 N.D. +0.2 +0.7 -0.6 N.D.
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_0.8%agar; Collection=outgrowth_LB; Time=7_days N.D. -0.7 +0.2 +0.8 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant N.D. +0.2 -0.5 +0.7 N.D.
phage 24hr PHYTO MOI 0.1 N.D. -0.6 +0.7 +0.6 N.D.
phage 36hr PHYTO6 MOI 0.1 N.D. -0.4 +0.1 +1.0 N.D.
phage 24hr PHYTO1 MOI 1 N.D. +0.3 +1.0 -0.3 N.D.
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_0.8%agar; Collection=outgrowth_LB; Time=7_days N.D. -0.4 +0.4 +1.1 N.D.
phage 36hr PHYTO2 MOI 1 N.D. -0.4 +0.8 +0.9 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. +0.3 +0.5 +0.7 N.D.
phage 36hr PHYTO10 MOI 10 N.D. +0.1 +0.3 +1.1 N.D.
phage 24hr PHYTO6 MOI 10 N.D. -0.4 +0.7 +1.1 N.D.
phage 24hr PHYTO4 MOI 10 N.D. -0.4 +1.0 +1.0 N.D.
nitrogen source Carnitine Hydrochloride (N) N.D. +0.4 +0.4 +0.8 N.D.
phage 36hr PHYTO8 MOI 10 N.D. +0.5 +0.4 +0.8 N.D.
phage 24hr PHYTO2 MOI 0.1 N.D. +0.4 +0.4 +1.3 N.D.
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