Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS26270 and BPHYT_RS26275 are separated by 143 nucleotidesBPHYT_RS26275 and BPHYT_RS26280 are separated by 145 nucleotidesBPHYT_RS26280 and BPHYT_RS26285 are separated by 152 nucleotidesBPHYT_RS26285 and BPHYT_RS26290 are separated by 265 nucleotides BPHYT_RS26270: BPHYT_RS26270 - hypothetical protein, at 1,464,743 to 1,464,925 _RS26270 BPHYT_RS26275: BPHYT_RS26275 - membrane protein, at 1,465,069 to 1,467,102 _RS26275 BPHYT_RS26280: BPHYT_RS26280 - hypothetical protein, at 1,467,248 to 1,467,427 _RS26280 BPHYT_RS26285: BPHYT_RS26285 - glycogen debranching protein, at 1,467,580 to 1,469,796 _RS26285 BPHYT_RS26290: BPHYT_RS26290 - photosystem reaction center subunit H, at 1,470,062 to 1,470,469 _RS26290
Group Condition BPHYT_RS26270 BPHYT_RS26275 BPHYT_RS26280 BPHYT_RS26285 BPHYT_RS26290
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. -0.5 +0.0 -0.9 -1.8
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth_LB; Time=7_days N.D. -0.4 -0.8 -0.7 -1.0
phage 36hr PHYTO7 MOI 0.1 N.D. +0.1 -0.9 -0.7 -1.0
phage 24hr PHYTO9 MOI 10 N.D. -0.1 -2.4 +0.7 -0.6
no phage control No phage control N.D. +0.1 -2.1 -0.4 +0.0
supernatant control:fungal media R2A with Vogels_fungal_media 0.8X N.D. -0.3 -0.7 -0.7 -0.6
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant N.D. +0.0 -0.6 -1.6 -0.1
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB N.D. +0.0 -0.2 -2.1 +0.1
carbon source 2-Deoxy-D-ribonic lithium salt (C) N.D. -0.9 +0.4 -0.2 -1.4
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. +0.2 -0.0 -1.1 -1.1
phage 24hr PHYTO MOI 0.1 N.D. +0.2 -1.8 +0.5 -0.7
supernatant control Vogels_fungal_media; pH 5.5 N.D. -0.9 -1.1 +0.4 +0.1
replicable vector efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 N.D. +0.2 -0.5 -1.9 +0.6
phage 36hr PHYTO7 MOI 1 N.D. -0.1 -1.2 +0.6 -0.6
lb_dap plate control and outgrow on gent LB_DAP agar plate, outgrowth in LB_gent N.D. +0.3 -0.5 -1.0 +0.4
phage 24hr PHYTO6 MOI 0.1 N.D. +0.3 -1.1 +0.7 -0.6
phage 36hr PHYTO6 MOI 10 N.D. -0.4 -0.7 +0.8 -0.4
phage 24hr PHYTO3 MOI 10 N.D. +0.9 -0.7 +0.0 -0.8
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. +0.6 +0.5 -0.2 -1.1
phage 24hr PHYTO11 MOI 0.1 N.D. -0.3 +0.8 +0.4 -1.0
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. -0.2 -0.8 +1.6 -0.4
in planta Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. -0.1 -0.6 -0.0 +1.7
in planta Plant=Zea_mays; PlantTreatment=None; Sample=soil; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. -0.1 +0.0 -0.6 +1.7
in planta Plant=Zea_mays; PlantTreatment=None; Sample=soil; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. +0.2 -0.1 -0.6 +1.7
in planta Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. +0.3 -0.4 -0.1 +1.5
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. +0.3 +0.1 +1.5 -0.6
phage 36hr PHYTO3 MOI 0.1 N.D. +0.1 -0.4 +1.1 +0.7
in planta Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. +0.1 +0.2 +0.2 +1.8
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.5X; growth supplemented with 0.4X LB N.D. -0.2 +1.5 -0.0 +1.0
in planta Plant=Zea_mays; PlantTreatment=None; Sample=soil; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. +0.1 -0.2 +0.6 +2.0
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