Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS26240 and BPHYT_RS26245 are separated by 259 nucleotidesBPHYT_RS26245 and BPHYT_RS26250 are separated by 51 nucleotidesBPHYT_RS26250 and BPHYT_RS26255 are separated by 16 nucleotidesBPHYT_RS26255 and BPHYT_RS26260 are separated by 117 nucleotides BPHYT_RS26240: BPHYT_RS26240 - hypothetical protein, at 1,458,184 to 1,458,480 _RS26240 BPHYT_RS26245: BPHYT_RS26245 - thioredoxin, at 1,458,740 to 1,459,060 _RS26245 BPHYT_RS26250: BPHYT_RS26250 - hypothetical protein, at 1,459,112 to 1,459,531 _RS26250 BPHYT_RS26255: BPHYT_RS26255 - lipase, at 1,459,548 to 1,460,570 _RS26255 BPHYT_RS26260: BPHYT_RS26260 - histidine kinase, at 1,460,688 to 1,462,856 _RS26260
Group Condition BPHYT_RS26240 BPHYT_RS26245 BPHYT_RS26250 BPHYT_RS26255 BPHYT_RS26260
phage 36hr PHYTO4 MOI 1 -0.8 N.D. -0.0 +0.1 -1.4
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.7 N.D. +0.0 -0.4 -0.9
phage 24hr PHYTO10 MOI 1 +0.1 N.D. -0.8 -0.2 -1.0
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=20h_outgrowth_LB; Time=7_days -0.9 N.D. -0.2 -0.3 -0.4
phage 24hr PHYTO3 MOI 10 -0.7 N.D. -0.7 +0.6 -0.8
nophagecontrol 36hr Phyto Lib Only +0.1 N.D. +0.1 -0.5 -1.3
phage 36hr PHYTO3 MOI 1 -0.9 N.D. +0.1 -0.2 -0.5
carbon source 2-Deoxy-D-ribonic lithium salt (C) -1.0 N.D. +0.3 -0.4 -0.4
phage 36hr PHYTO2 MOI 1 -0.2 N.D. -0.8 +0.2 -0.6
phage P. phyto corn MOI 1 +0.4 N.D. -0.5 -0.4 -0.9
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth; Time=14_days -1.1 N.D. +0.3 -0.1 -0.5
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -1.2 N.D. -0.1 +0.7 -0.7
phage 24hr PHYTO11 MOI 0.1 -0.3 N.D. +0.4 -0.0 -1.1
vitamin With vitamins t1 -1.4 N.D. +0.3 -0.1 +0.2
phage P. phyto PHYTO2 MOI 1 +0.7 N.D. -0.1 -0.1 -1.4
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 1X +0.5 N.D. -0.9 -0.4 -0.1
phage 24hr PHYTO MOI 0.1 -0.6 N.D. +0.4 +0.3 -0.5
phage 36hr PHYTO3 MOI 1 +0.1 N.D. +0.2 +0.6 -1.1
phage 36hr PHYTO8 MOI 10 +0.6 N.D. +0.1 -0.2 -0.7
phage 36hr PHYTO2 MOI 1 +0.3 N.D. -0.0 +0.5 -0.9
carbon source Tween 20 (C) -0.6 N.D. +0.3 +0.7 -0.2
nophagecontrol 36hr Phyto Lib only -0.9 N.D. +0.1 +0.4 +0.7
phage 24hr PHYTO3 MOI 10 +1.0 N.D. +0.2 +0.3 -1.1
lb_dap plate control and outgrow on gent LB_DAP agar plate, outgrowth in LB_gent +0.4 N.D. +0.5 +0.5 -0.4
phage 36hr PHYTO1 MOI 1 +0.7 N.D. +0.7 +0.4 -0.6
tn7 efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pRH19; outgrowth in LB_gent50 +0.7 N.D. -0.3 +0.3 +0.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.2 N.D. +0.7 +0.1 +0.9
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.7 N.D. +0.7 -0.0 +0.3
carbon source Deoxyribonucleic from herring sperm 10 mg/mL (C) +0.6 N.D. +0.6 -0.2 +0.8
temperature LB 37C +0.1 N.D. +0.2 -0.1 +1.8
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