Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS26200 and BPHYT_RS26205 are separated by 13 nucleotidesBPHYT_RS26205 and BPHYT_RS26210 are separated by 689 nucleotidesBPHYT_RS26210 and BPHYT_RS26215 are separated by 93 nucleotidesBPHYT_RS26215 and BPHYT_RS26220 are separated by 15 nucleotides BPHYT_RS26200: BPHYT_RS26200 - acetate kinase, at 1,448,974 to 1,450,134 _RS26200 BPHYT_RS26205: BPHYT_RS26205 - phosphoketolase, at 1,450,148 to 1,452,541 _RS26205 BPHYT_RS26210: BPHYT_RS26210 - ankyrin, at 1,453,231 to 1,453,767 _RS26210 BPHYT_RS26215: BPHYT_RS26215 - haloacid dehalogenase, at 1,453,861 to 1,454,553 _RS26215 BPHYT_RS26220: BPHYT_RS26220 - 6-phosphogluconate dehydrogenase, at 1,454,569 to 1,455,606 _RS26220
Group Condition BPHYT_RS26200 BPHYT_RS26205 BPHYT_RS26210 BPHYT_RS26215 BPHYT_RS26220
phage 36hr PHYTO2 MOI 1 -1.5 -0.5 N.D. -0.8 +0.4
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.7 -0.1 N.D. -0.9 -0.0
phage 24hr PHYTO9 MOI 10 -0.5 +0.4 N.D. -1.3 -0.1
phage 24hr PHYTO11 MOI 1 -1.2 +0.0 N.D. -0.3 +0.1
phage 24hr PHYTO1 MOI 1 -0.4 +0.3 N.D. -1.0 -0.1
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth; Time=14_days -1.5 +0.1 N.D. +0.1 +0.1
phage PHYTO3 MOI 0.1 -0.3 +0.1 N.D. -1.4 +0.3
phage 24hr PHYTO2 MOI 1 -1.0 +0.4 N.D. -0.6 +0.1
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days -0.6 +0.3 N.D. -0.8 +0.1
carbon source Phenylacetic 5 mM (C) -1.0 -0.2 N.D. +0.4 +0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.4 +0.7 N.D. -0.8 -0.1
phage 36hr PHYTO3 MOI 1 -0.6 +0.2 N.D. -0.6 +0.4
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB -0.9 +0.6 N.D. +0.2 -0.4
phage 24hr PHYTO4 MOI 10 -1.1 +0.3 N.D. +0.0 +0.3
phage 24hr PHYTO3 MOI 1 -0.2 +0.6 N.D. -0.8 +0.2
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days -0.4 +0.3 N.D. +0.6 -0.4
phage P. phyto corn MOI 1 -1.0 +0.4 N.D. +0.7 +0.0
in planta Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days +0.8 +0.2 N.D. -0.3 -0.3
in planta Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 -0.9 +0.7 N.D. +1.0 -0.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant +0.8 +0.2 N.D. -0.4 -0.3
stress Nickel (II) chloride 0.5 mM +0.2 +0.6 N.D. -0.6 +0.2
carbon source Malonic (C) -0.4 -0.2 N.D. +0.9 +0.2
carbon source Protocatechuic Acid (C) +1.0 +0.1 N.D. -0.8 +0.4
phage 36hr PHYTO10 MOI 10 +0.7 +0.2 N.D. -0.6 +0.3
phage 24hr PHYTO MOI 0.1 +0.8 +0.1 N.D. -0.6 +0.5
carbon source Tween 20 (C) +0.7 +0.4 N.D. -0.2 +0.4
carbon source adipate (C) +1.1 +0.3 N.D. -0.3 +0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant +0.8 +0.4 N.D. -0.2 +0.5
in planta Plant=Arabidopsis_thaliana_FLS2; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_0.8%agar; Collection=outgrowth_LB; Time=7_days +0.8 -0.3 N.D. +0.8 +0.3
carbon source Suberic (C) +1.3 +0.4 N.D. -0.1 +0.1
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