Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS26145 and BPHYT_RS26150 are separated by 428 nucleotidesBPHYT_RS26150 and BPHYT_RS26155 are separated by 45 nucleotidesBPHYT_RS26155 and BPHYT_RS26160 are separated by 38 nucleotidesBPHYT_RS26160 and BPHYT_RS26165 are separated by 56 nucleotides BPHYT_RS26145: BPHYT_RS26145 - hypothetical protein, at 1,441,279 to 1,441,797 _RS26145 BPHYT_RS26150: BPHYT_RS26150 - hypothetical protein, at 1,442,226 to 1,442,996 _RS26150 BPHYT_RS26155: BPHYT_RS26155 - carboxylesterase, at 1,443,042 to 1,443,911 _RS26155 BPHYT_RS26160: BPHYT_RS26160 - membrane protein, at 1,443,950 to 1,444,756 _RS26160 BPHYT_RS26165: BPHYT_RS26165 - hypothetical protein, at 1,444,813 to 1,445,517 _RS26165
Group Condition BPHYT_RS26145 BPHYT_RS26150 BPHYT_RS26155 BPHYT_RS26160 BPHYT_RS26165
phage 24hr PHYTO2 MOI 1 N.D. -0.9 -0.5 -2.3 N.D.
phage 24hr PHYTO3 MOI 1 N.D. -1.3 -0.5 -0.6 N.D.
phage 24hr PHYTO11 MOI 0.1 N.D. -0.2 -0.8 -1.5 N.D.
tn7 efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pRH19; outgrowth in LB_gent50 N.D. -0.2 -0.1 -1.8 N.D.
phage 24hr PHYTO3 MOI 10 N.D. -0.9 -0.6 -0.6 N.D.
stress Bacitracin 0.25 mg/ml N.D. -0.8 -0.7 -0.6 N.D.
phage 36hr PHYTO10 MOI 1 N.D. -0.0 -1.2 -0.7 N.D.
phage 24hr PHYTO MOI 0.1 N.D. -0.5 -0.6 -0.7 N.D.
phage 24hr PHYTO10 MOI 1 N.D. -0.3 -0.6 -0.8 N.D.
phage P. phyto PHYTO2 MOI 1 N.D. -0.1 -1.2 -0.4 N.D.
phage 24hr PHYTO6 MOI 10 N.D. -0.3 -0.1 -1.3 N.D.
motility outer cut, LB soft agar motility assay N.D. -0.5 -0.9 -0.3 N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. +0.4 -0.3 -1.7 N.D.
phage 36hr PHYTO3 MOI 1 N.D. -0.8 -0.7 +0.5 N.D.
phage 36hr PHYTO1 MOI 1 N.D. -0.5 -0.7 +0.4 N.D.
phage 36hr PHYTO2 MOI 0.1 N.D. -0.4 -0.9 +0.4 N.D.
in planta Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days N.D. -0.8 -0.4 +0.5 N.D.
phage 36hr PHYTO2 MOI 1 N.D. -0.5 -0.7 +0.5 N.D.
phage 36hr PHYTO8 MOI 10 N.D. -0.7 -0.4 +0.7 N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. +0.7 -0.7 -0.3 N.D.
phage 24hr PHYTO9 MOI 10 N.D. -0.4 -0.5 +0.8 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. +0.8 -0.5 -0.4 N.D.
stress Lomefloxacin 0.001 mM N.D. -0.3 -0.4 +0.9 N.D.
phage 36hr PHYTO9 MOI 0.1 N.D. -0.3 -0.5 +0.9 N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. +0.6 -0.7 +0.3 N.D.
phage 36hr PHYTO8 MOI 0.1 N.D. -0.1 -0.6 +1.1 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant N.D. -0.0 -0.9 +1.3 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant N.D. +0.1 -0.7 +1.2 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant N.D. +0.5 -0.3 +0.9 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant N.D. +0.6 +0.3 +0.9 N.D.
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